ACC oxidase (ACO) genes in Trifolium occidentale (L.) and their relationship to ACO genes in white clover (T. repens L.) and T. pallescens (L.)

Du, Zhenning; Leung, Susanna; Dorling, Sarah J.; McManus, Michael T.

doi:10.1016/j.plaphy.2011.01.017

Cited by 3 publications

(2 citation statements)

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“…To obtain the 3′ untranslated region (UTR) sequences, PCR was performed using a 3′‐RACE adapter primer and specific primers designed for each sequence (Table S1) using the method as outlined in Du et al . (). The 5′‐UTR and promoter sequences were obtained using the GenomeWalker ™ Universal Kit protocol (Clontech, Mountain View, CA, USA).…”

Section: Methodsmentioning

confidence: 97%

“…The PCR products were sequenced and aligned, and the sequences were used to design gene-specific primers for 3 0rapid amplification of cDNA ends (RACE) and 5 0 -RACE to obtain the full coding region (GenBank accessions: KFO22195-198;KFO22203-22210). To obtain the 3 0 untranslated region (UTR) sequences, PCR was performed using a 3 0 -RACE adapter primer and specific primers designed for each sequence (Table S1) using the method as outlined in Du et al (2011). The 5 0 -UTR and promoter sequences were obtained using the GenomeWalker TM Universal Kit protocol (Clontech, Mountain View, CA, USA).…”

Section: Isolation Of Tr-kpi Genesmentioning

confidence: 99%

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Knock‐down of transcript abundance of a family ofKunitz proteinase inhibitorgenes in white clover (Trifolium repens) reveals a redundancy and diversity of gene function

et al. 2015

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SummaryThe transcriptional regulation of four phylogenetically distinct members of a family of Kunitz proteinase inhibitor (KPI) genes isolated from white clover (Trifolium repens; designated Tr-KPI1, Tr-KPI2, Tr-KPI4 and Tr-KPI5) has been investigated to determine their wider functional role.The four genes displayed differential transcription during seed germination, and in different tissues of the mature plant, and transcription was also ontogenetically regulated. Heterologous over-expression of Tr-KPI1, Tr-KPI2, Tr-KPI4 and Tr-KPI5 in Nicotiana tabacum retarded larval growth of the herbivore Spodoptera litura, and an increase in the transcription of the pathogenesis-related genes PR1 and PR4 was observed in the Tr-KPI1 and Tr-KPI4 overexpressing lines.RNA interference (RNAi) knock-down lines in white clover displayed significantly altered vegetative growth phenotypes with inhibition of shoot growth and a stimulation of root growth, while knock-down of Tr-KPI1, Tr-KPI2 and Tr-KPI5 transcript abundance also retarded larval growth of S. litura. Examination of these RNAi lines revealed constitutive stress-associated phenotypes as well as altered transcription of cellular signalling genes.These results reveal a functional redundancy across members of the KPI gene family. Further, the regulation of transcription of at least one member of the family, Tr-KPI2, may occupy a central role in the maintenance of a cellular homeostasis.

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Section: Methodsmentioning

confidence: 97%

Section: Isolation Of Tr-kpi Genesmentioning

confidence: 99%