2008
DOI: 10.1021/bp049609k
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Accelerated Biocatalyst Stability Testing for Process Optimization

Abstract: The deactivation of protein biocatalysts even at relatively low temperatures is one of the principal drawbacks to their use. To aid in the development of novel biocatalysts, we have derived an equation for both time- and temperature-dependent activity of the biocatalyst based on known concepts such as transition state theory and the Lumry-Eyring model. We then derived an analytical solution for the total turnover number (ttn), under isothermal operation, as a function of the catalytic constant kcat, the unfold… Show more

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Cited by 29 publications
(33 citation statements)
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“…The temperature dependency of all reactions is modeled according to the Arrhenius law (Gibbs et al, 2005). The used formulation of the Arrhenius law is mathematically equivalent to the conventional formulation but numerically more convenient (Bock et al, 2004):…”
Section: Modelingmentioning
confidence: 99%
See 1 more Smart Citation
“…The temperature dependency of all reactions is modeled according to the Arrhenius law (Gibbs et al, 2005). The used formulation of the Arrhenius law is mathematically equivalent to the conventional formulation but numerically more convenient (Bock et al, 2004):…”
Section: Modelingmentioning
confidence: 99%
“…These long-term experiments are not compatible with high throughput screening approaches. Boy et al (1999Boy et al ( , 2001 and Gibbs et al (2005) propose an elegant approach to determine the long term enzyme stability within a short time. Similar to the breaking tests employed in material science, the enzyme behavior is observed under partly extreme conditions to predict the long term behavior under more moderate operation conditions.…”
Section: Introductionmentioning
confidence: 99%
“…There are other kinetic models for enzyme deactivation used in the literature like Lumry-Eyring model or other [26] used both for glucoamylase deactivation as well as for other enzymes [27,28]. This study is focused on determining the enzyme stability at different operating conditions, and, therefore, the simplest model [29] was chosen to describe the enzyme deactivation rate and it was called the overall operational stability decay rate.…”
Section: Mass Balances For Various Reactor Typesmentioning
confidence: 99%
“…Consequently the time-dependent decrease of active enzyme concentration c E can be expressed as a function of the equilibrium constant K eq and the rate constant k i for irreversible deactivation (Gibbs et al, 2005):…”
Section: Enzyme Activation and Deactivation Parameter Estimationmentioning
confidence: 99%
“…Temperature-dependence of the irreversible denaturation of the unfolded enzyme U can be described by Gibbs et al (2005).…”
Section: Enzyme Activation and Deactivation Parameter Estimationmentioning
confidence: 99%