Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes

Fujita, Hideaki; Nedachi, Taku; Kanzaki, Makoto

doi:10.1016/j.yexcr.2007.03.002

Cited by 203 publications

(210 citation statements)

References 67 publications

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“…Muscle cells were stimulated for 120 min (18 V; 1 Hz; 24 ms) at 37°C in 5% CO 2 in a humidified chamber according to methods previously described [26]. To verify that cells were contracting, we filmed the cells.…”

Section: Methodsmentioning

confidence: 99%

Brain-derived neurotrophic factor is produced by skeletal muscle cells in response to contraction and enhances fat oxidation via activation of AMP-activated protein kinase

Matthews¹,

Åström

Chan³

et al. 2009

Diabetologia

572

480

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Aims/hypothesis Brain-derived neurotrophic factor (BDNF) is produced in skeletal muscle, but its functional significance is unknown. We aimed to determine the signalling processes and metabolic actions of BDNF. Methods We first examined whether exercise induced BDNF expression in humans. Next, C2C12 skeletal muscle cells were electrically stimulated to mimic contraction. L6 myotubes and isolated rat extensor digitorum longus muscles were treated with BDNF and phosphorylation of the proteins AMP-activated protein kinase (AMPK) (Thr 172 ) and acetyl coenzyme A carboxylase β (ACCβ) (Ser 79 ) were analysed, as was fatty acid oxidation (FAO).Finally, we electroporated a Bdnf vector into the tibialis cranialis muscle of mice. Results BDNF mRNA and protein expression were increased in human skeletal muscle after exercise, but muscle-derived BDNF appeared not to be released into the circulation. Bdnf mRNA and protein expression was increased in muscle cells that were electrically stimulated. BDNF increased phosphorylation of AMPK and ACCβ and enhanced FAO both in vitro and ex vivo. The effect of BDNF on FAO was AMPK-dependent, since the increase in FAO was abrogated in cells infected with an AMPK dominant negative adenovirus or treated with Compound C, an inhibitor of AMPK. Electroporation of a Bdnf expression

show abstract

Section: Methodsmentioning

confidence: 99%

Brain-derived neurotrophic factor is produced by skeletal muscle cells in response to contraction and enhances fat oxidation via activation of AMP-activated protein kinase

Matthews¹,

Åström

Chan³

et al. 2009

Diabetologia

572

480

View full text Add to dashboard Cite

show abstract

“…S4), further optimization of these processes in vitro will be required to allow the efficient survival and functional integration of tissue constructs in the host neuromuscular system. The use of angiogenic and/or neurotrophic factors, 7,30 electrical and mechanical stimulation, 9,16,31 and coculture with vascular and/or neuronal cells 6,32,33 are just some of the potential strategies to achieve this goal. Development of improved immunosuppression regimes 34 and cell sources with robust regenerative capacity (e.g., from human pluripotent stem cells 35 ) are some of the other important milestones toward the therapeutic use of tissue engineering to restore lost muscle function.…”

Section: Discussionmentioning

confidence: 99%

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

Bian

Juhas

Pfeiler

et al. 2012

Tissue Engineering Part A

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The field of skeletal muscle tissue engineering is currently hampered by the lack of methods to form large muscle constructs composed of dense, aligned, and mature myofibers and limited understanding of structure-function relationships in developing muscle tissues. In our previous studies, engineered muscle sheets with elliptical pores (''muscle networks'') were fabricated by casting cells and fibrin gel inside elastomeric tissue molds with staggered hexagonal posts. In these networks, alignment of cells around the elliptical pores followed the local distribution of tissue strains that were generated by cell-mediated compaction of fibrin gel against the hexagonal posts. The goal of this study was to assess how systematic variations in pore elongation affect the morphology and contractile function of muscle networks. We found that in muscle networks with more elongated pores the force production of individual myofibers was not altered, but the myofiber alignment and efficiency of myofiber formation were significantly increased yielding an increase in the total contractile force despite a decrease in the total tissue volume. Beyond a certain pore length, increase in generated contractile force was mainly contributed by more efficient myofiber formation rather than enhanced myofiber alignment. Collectively, these studies show that changes in local tissue geometry can exert both direct structural and indirect myogenic effects on the functional output of engineered muscle. Different hydrogel formulations and pore geometries will be explored in the future to further augment contractile function of engineered muscle networks and promote their use for basic structure-function studies in vitro and, eventually, for efficient muscle repair in vivo.

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“…The stimulator was set at a pulse voltage of 20 V, pulse frequency of 1 Hz, pulse duration of 2 ms, pulse train of 115 min, and 5-min of rest, as described previouly (Fujita et al 2007). The cells in C-Dishes were maintained in a 5% CO 2 incubator at 37°C.…”

Section: Electrical Cell Stimulationmentioning

confidence: 99%

Electric Pulse Stimulation Induces NMDA Glutamate Receptor mRNA in NIH3T3 Mouse Fibroblasts

Okutsu

Hatakeyama

Kanazaki

et al. 2008

Tohoku J. Exp. Med.

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Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes

Cited by 203 publications

References 67 publications

Brain-derived neurotrophic factor is produced by skeletal muscle cells in response to contraction and enhances fat oxidation via activation of AMP-activated protein kinase

Brain-derived neurotrophic factor is produced by skeletal muscle cells in response to contraction and enhances fat oxidation via activation of AMP-activated protein kinase

Local Tissue Geometry Determines Contractile Force Generation of Engineered Muscle Networks

Electric Pulse Stimulation Induces NMDA Glutamate Receptor mRNA in NIH3T3 Mouse Fibroblasts

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