Accelerated differentiation of bone marrow-derived dendritic cells in atopic prone mice

Koike, Eiko; Takano, Hirohisa; Inoue, Ken‐ichiro; Yanagisawa, Rie

doi:10.1016/j.intimp.2008.08.006

Cited by 11 publications

(14 citation statements)

References 27 publications

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“…Levels of IL-4 (Amersham, Buckinghamshire, UK), IL-5 (Endogen, Cambridge, MA), IL-13 (R&D Systems, Minneapolis, MN), and IFN-c (R&D Systems) were measured by ELISA according to the manufacturer's instructions as previously conducted (22,25) (n ¼ 6 for IL-4, n ¼ 14-15 in each group, respectively).…”

Section: Methodsmentioning

confidence: 99%

Effects of Diesel Exhaust Particles on Antigen-Presenting Cells and Antigen-Specific Th Immunity in Mice

Inoue

Koike

Takano

et al. 2009

Exp Biol Med (Maywood)

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Diesel exhaust particles (DEP) exacerbate antigen-related airway inflammation and hyperresponsiveness in mice; however, the mechanisms remain undefined. The present study characterized more precisely which pathways and cellular events of the allergic response are amplified by DEP in view of the maturation/activation/function of antigen-presenting cells (APC) and the antigen-specific Th response. We evaluated the effects of DEP on the phenotype and function of bone marrow-derived dendritic cells (BMDC) in vitro and on the expression pattern of APC-related molecules in the murine lung in the presence or absence of antigen in vivo. Also, we tested the effects of in vivo DEP co-exposure with antigen on the splenic antigen-specific Th response in the context of cytokine production. DEP significantly increased both allogeneic and antigen (ovalbumin: OVA)-specific syngeneic T-cell proliferation in vitro. In addition, an in vivo experiment showed that repetitive pulmonary exposure to DEP plus antigen (OVA) increased the numbers of MHC class II+cells and those expressing CD11c, DEC205 (DC markers), CD80, CD86 (co-stimulatory molecules), F4/80 (a macrophage marker), and CD19 (a B-cell differentiation antigen) in the lung as compared to that of others (vehicle, DEP, or OVA). Furthermore, an ex vivo assay system demonstrated that splenic mononuclear cells primed by DEP plus OVA produced a greater amount of interleukin (IL)-4, IL-5, and IL-13 after in vitro antigen stimulation compared to those primed by the other treatments. In conclusion, enhancement of allergic responses by DEP can be explained via two novel mechanisms, i.e., enhancement effects on APC including DC and on antigen-specific Th response, which culminate in the promotion of local and systemic dysregulated Th immunity.

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Section: Methodsmentioning

confidence: 99%

Effects of Diesel Exhaust Particles on Antigen-Presenting Cells and Antigen-Specific Th Immunity in Mice

Inoue

Koike

Takano

et al. 2009

Exp Biol Med (Maywood)

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“…The preparation of bone marrow cells was conducted as previously described [22,23]. BMDC were differentiated using a modified protocol of Lutz et al [24], as previously conducted [22,23].…”

Section: Preparation and Generation Of Bone Marrow-derived Dendritic mentioning

confidence: 99%

“…BMDC were differentiated using a modified protocol of Lutz et al [24], as previously conducted [22,23]. …”

Section: Preparation and Generation Of Bone Marrow-derived Dendritic mentioning

confidence: 99%

“…OVA/Alum-sensitized T cells were derived from a pool of spleens from OVA/Alum-sensitized syngeneic mice, as previously described [22,23]. On day 8 of generation for BMDC, BMDC (from 2.5 × 10 3 to 1 × 10 4 ) and OVA/Alum-specific T-cells (2 × 10 5 ) were co-cultured in the presence of OVA (40 μg) for 2 days, and T-cell proliferation was measured using a Cell-Proliferation-ELISA Kit (Roche Molecular Biochemicals, Mannheim, Germany), as previously described [23].…”

Section: Preparation Of Allergen-sensitized T Cells and Allergen-specmentioning

confidence: 99%

“…On day 8 of generation for BMDC, BMDC (from 2.5 × 10 3 to 1 × 10 4 ) and OVA/Alum-specific T-cells (2 × 10 5 ) were co-cultured in the presence of OVA (40 μg) for 2 days, and T-cell proliferation was measured using a Cell-Proliferation-ELISA Kit (Roche Molecular Biochemicals, Mannheim, Germany), as previously described [23]. This experiment was repeated three times using three animals in each group.…”

Section: Preparation Of Allergen-sensitized T Cells and Allergen-specmentioning

confidence: 99%

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Peroxiredoxin I is a negative regulator of Th2-dominant allergic asthma

Inoue¹,

Takano²,

Koike³

et al. 2009

International Immunopharmacology

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Repeated pulmonary exposure to single-walled carbon nanotubes exacerbates allergic inflammation of the airway: Possible role of oxidative stress

Inoue

Yanagisawa

Koike

et al. 2010

Free Radical Biology and Medicine

116

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Accelerated differentiation of bone marrow-derived dendritic cells in atopic prone mice

Cited by 11 publications

References 27 publications

Effects of Diesel Exhaust Particles on Antigen-Presenting Cells and Antigen-Specific Th Immunity in Mice

Effects of Diesel Exhaust Particles on Antigen-Presenting Cells and Antigen-Specific Th Immunity in Mice

Peroxiredoxin I is a negative regulator of Th2-dominant allergic asthma

Repeated pulmonary exposure to single-walled carbon nanotubes exacerbates allergic inflammation of the airway: Possible role of oxidative stress

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