Accelerated fabrication of antibacterial and osteoinductive electrospun fibrous scaffolds via electrochemical deposition

Abstract

Electrospun fibrous scaffolds have attracted much research interest due to their many applications in orthopedics and other relevant fields. However, poor surface bioactivity of the polymer scaffold body significantly limits the implementation of many potential applications, and an effective solution remains a great challenge for researchers. Herein, a highly efficient method, namely pulsed electrochemical deposition (ED) with co-electrospinning nano-Ag dopant, to fabricate poly(L-lactic acid) (PLLA)/nano-Ag composite fibers is presented. The resulting product demonstrated excellent antibacterial properties, as well as strong capabilities in facilitating the precipitation of calcium phosphate crystals at fiber surfaces and in promoting osteogenic differentiation. In the process of ED, the conductivity of the fibers was observed to increase due to the nano-Ag dopant. Upon applying pulse signals when charging, water electrolysis occurred in micro-reactive regions of anodic fibers, forming OH À , an alkaline environment that allowed the supersaturation of calcium phosphate. When discharging, the calcium phosphate in the solution diffused rapidly and reduced the concentration polarization, reforming a homogeneous electrolyte. The realization of efficient bioactive coatings at fiber surfaces was achieved in a highly efficient manner by repeating the above charging and discharging processes. Therefore, ED can be adopted to simplify and accelerate the fabrication process of an osteogenetic and antibacterial electrospun fibrous scaffold.

Bi-layered nanofibrous membrane with osteogenic and antibacterial functions for periodontal tissue regeneration

“…S. aureus and E. coli 16,39 were used to determine the antibacterial capacity of the AL (nAg-PLGA/gelatin nanofibrous membranes) of the bi-layered nanofibrous membrane, with PLGA/gelatin nanofibrous membranes as controls. As shown in Figure 8, the bacterial inhibition zone was observed around nAg-PLGA/gelatin nanofibrous membranes, but no inhibition zone was observed around PLGA/gelatin nanofibrous membranes.…”

Scaffolds in the microbial resistant era: Fabrication, materials, properties and tissue engineering applications

“…Polymeric PLA scaffolds with metals such as silver, which give a final result of antibacterial activity, showed osteogenic differentiation and no cytotoxicity to human cells [ 179 ]. A continuous Ag + release can last more than 3 weeks, which can be useful in long-term bone implants.…”

Drug‐Eluting Medical Textiles: From Fiber Production and Textile Fabrication to Drug Loading and Delivery

“…Using this loading method, the drug is incorporated on the surface of the fiber by immersing in a drug solution [171] or by coating with drug encapsulated micro-or nanoparticles [183] (Figure 5A). Several coating methods, such as electrochemical deposition, [184,185] immersion, [170] dispersion, and curtain coating [132] are used for drug incorporation onto the fibers. Coating parameters such as pH, solids content, and coating thickness can be tuned to reach the desired coating.…”