Accelerated Fourier ptychographic diffraction tomography with sparse annular <scp>LED</scp> illuminations

Zhou, Shun; Li, Jiaji; Sun, Jiasong; Zhou, Ning; Chen, Qian; Zuo, Chao

doi:10.1002/jbio.202100272

Cited by 20 publications

(4 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The adopted annular matched illumination configuration could provide a reliable and efficient approach for aberration correction on the transparent biospecimen. Moreover, as an extension to FPM, the technology of 3D FPM [68][69][70][71] is developed to further reveal the 3D phase information for thick samples. The existence of aberrations could also affect the imaging performance of the 3D phase imaging.…”

Section: Discussionmentioning

confidence: 99%

Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

et al. 2022

View full text Add to dashboard Cite

Quantitative phase imaging (QPI) has emerged as a valuable tool for biomedical research thanks to its unique capabilities for quantifying optical thickness variation of living cells and tissues. Among many QPI methods, Fourier ptychographic microscopy (FPM) allows long-term label-free observation and quantitative analysis of large cell populations without compromising spatial and temporal resolution. However, high spatio-temporal resolution imaging over a long-time scale (from hours to days) remains a critical challenge: optically inhomogeneous structure of biological specimens as well as mechanical perturbations and thermal fluctuations of the microscope body all result in time-varying aberration and focus drifts, significantly degrading the imaging performance for long-term study. Moreover, the aberrations are sample- and environment-dependent, and cannot be compensated by a fixed optical design, thus necessitating rapid dynamic correction in the imaging process. Here, we report an adaptive optical QPI method based on annular illumination FPM. In this method, the annular matched illumination configuration (i.e., the illumination numerical aperture (NA) strictly equals to the objective NA), which is the key for recovering low-frequency phase information, is further utilized for the accurate imaging aberration characterization. By using only 6 low-resolution images captured with 6 different illumination angles matching the NA of a 10x, 0.4 NA objective, we recover high-resolution quantitative phase images (synthetic NA of 0.8) and characterize the aberrations in real time, restoring the optimum resolution of the system adaptively. Applying our method to live-cell imaging, we achieve diffraction-limited performance (full-pitch resolution of $$655\,nm$$ 655 n m at a wavelength of $$525\,nm$$ 525 n m ) across a wide field of view ($$1.77\,mm^2$$ 1.77 m m 2 ) over an extended period of time.

show abstract

Section: Discussionmentioning

confidence: 99%

Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

et al. 2022

View full text Add to dashboard Cite

show abstract

“…As a light source, an annular LED array (central wavelength λ = 520 nm, 1586, Adafruit, USA) was used for the annular plane wave illuminations at various azimuthal angles. Recently, several researchers have shown that annular illuminations with the incident angle matching the objective NA (𝑁𝐴 𝑜𝑏𝑗 ) is the optimal condition for tomographic imaging 13,[62][63][64] . This illumination scheme optimally encodes both low-and high-spatial frequency information across the entire 3D volume using a small number of intensity measurements.…”

Section: Methodsmentioning

confidence: 99%

Polarization-sensitive intensity diffraction tomography

Joo

Song

Kim

et al. 2022

Preprint

View full text Add to dashboard Cite

Optical anisotropy, which is an intrinsic property of many materials, originates from the structural arrangement of molecular structures, and to date, various polarization-sensitive imaging (PSI) methods have been developed to investigate the nature of anisotropic materials. In particular, the recently developed tomographic PSI technologies enable the investigation of anisotropic materials through volumetric mappings of the anisotropy distribution of these materials. However, these reported methods mostly operate on a single scattering model, and are thus not suitable for three-dimensional (3D) PSI imaging of multiple scattering samples. Here, we present a novel reference-free 3D polarization-sensitive computational imaging technique—polarization-sensitive intensity diffraction tomography (PS-IDT)—that enables the reconstruction of 3D anisotropy distribution of both weakly and multiple scattering specimens from multiple intensity-only measurements. A 3D anisotropic object is illuminated by circularly polarized plane waves at various illumination angles to encode the isotropic and anisotropic structural information into 2D intensity information. These information are then recorded separately through two orthogonal analyzer states, and a 3D Jones matrix is iteratively reconstructed based on the vectorial multi-slice beam propagation model and gradient descent method. We demonstrate the 3D anisotropy imaging capabilities of PS-IDT by presenting 3D anisotropy maps of various samples, including potato starch granules and tardigrade.

show abstract

“…Fourier ptychography imaging draws on the idea of synthetic aperture [1] , using LED arrays of different positions for imaging. Acquiring multiple low-resolution images containing different spectrum components, Fourier ptychography use the phase recovery technology, and finally obtains a high-resolution, large field of view imaging results [2][3][4][5][6][7][8] . The phase recovery process is essentially an inverse problem solved optimally.…”

Section: Introductionmentioning

confidence: 99%

Sparsely sampled Fourier ptychography in diffuse reflection

2023

J. Phys.: Conf. Ser.

View full text Add to dashboard Cite

Fourier ptychography microscopy combines the idea of aperture synthesis, which enables the synthesis of multiple low-resolution images in the frequency domain to achieve large field-of-view and high-resolution imaging results. The Fourier ptychography imaging technique applied to the field of far-field diffuse reflectance imaging needs to consider the impact of diffuse reflectance speckle formed by the object’s rough surface on the imaging results. Therefore, far-field diffuse reflectance Fourier ptychography often requires more than microscopic imaging of low-resolution images to achieve super-resolution. In response, we design a sparse sampling method that can reduce the number of required low-resolution images to one-fourth of the original sampling, and does not affect the imaging resolution. Experimental results show that our method can significantly improve imaging efficiency and achieve super-resolution far-field imaging with fast sampling.

show abstract

Accelerated Fourier ptychographic diffraction tomography with sparse annular LED illuminations

Cited by 20 publications

References 62 publications

Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

Adaptive optical quantitative phase imaging based on annular illumination Fourier ptychographic microscopy

Polarization-sensitive intensity diffraction tomography

Sparsely sampled Fourier ptychography in diffuse reflection

Contact Info

Product

Resources

About