Accelerated healing of duodenal ulcers by oral administration of a mutein of basic fibroblast growth factor in rats

Szabó, Sándor; Folkman, Judah; Vattay, P; Morales, Romeo E.; Pinkus, Geraldine S.; Kato, Koichi

doi:10.1016/0016-5085(94)90773-0

Cited by 139 publications

(79 citation statements)

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“…Healing of erosions or ulcers starts with epithelial hyperplasia accompanied by congestion and proliferation of capillaries (angiogenesis) in the lamina propria (Murray 1999). Angiogenesis has been shown to be pivotal for healing of duodenal ulcers in rats (Szabo et al 1994). When horses spent 16 days on pasture after being fed alfalfa chaff, median lesion score of the antrum decreased from 2 to 0.5.…”

Section: Figmentioning

confidence: 99%

Effects of alfalfa chaff on the gastric mucosa in adult horses

Vondran¹,

Venner²,

Coenen³

et al. 2017

PHK

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Summary: Feeding alfalfa hay is often recommended because of its buffering properties associated with substances, like protein and calcium, to prevent lesions. However, the particle size of the alfalfa preparations seems to have an influence on gastric mucosa in weanlings. We hypothesized that feeding alfalfa chaff with harsh particles would negatively impact glandular gastric mucosa in adult horses under maintenance conditions. The study was performed as a cross-over 2 × 2 Latin square design with 16-days washout intervals over 8 weeks. Six geldings (19 to 21 years of age) were kept in individual boxes (10.85 m 2 ). The horses were fed 1.5 kg per 100 kg BW of alfalfa chaff or grass hay, offered twice a day. Horses were subjected to a gastroscopy immediately before the feeding period (T= 0), after the first feeding period of 12 days (T =12), after the first washout period (T = 28), after the second feeding period of 12 days (T = 40) and after the second washout period (T = 56). When horses were fed alfalfa chaff the median score of the antrum raised from 0 to 2 (p = 0.07). And after the washout period the median score of the antrum decreased to 0.5 (p = 0.04). In conclusion feeding alfalfa chaff induced glandular mucosal lesions at the antrum. Lesions of the antrum induced by feeding alfalfa chaff seem to be reversible and to heal within 16 days. Keywords: alfalfa, particle size, gastric lesions, gastroscopy, EGGD, nutrition Citation: Vondran S., Venner M. , Coenen M., Vervuert I. (2017) Effects of alfalfa chaff on the gastric mucosa in adult horses. Pferdeheilkunde 33, 66-71;

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Section: Figmentioning

confidence: 99%

Effects of alfalfa chaff on the gastric mucosa in adult horses

Vondran¹,

Venner²,

Coenen³

et al. 2017

PHK

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“…Prolonged inhibition of gastric acidity and reduction of bFGF level could increase the expression of bFGF in the gastric mucosa at the late stage of protamine sulphate treatment. 33,37,42 Other factors, such as an increase in nitric oxide by protamine sulphate could counteract the initial adverse action resulting from the reduction of bFGF at the early phase of protamine sulphate treatment. Whether or not stimulation of bFGF level at 7 days after protamine sulphate treatment participated in the healing process of gastric ulcer is still unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Further study is needed to af®rm this bene®cial action of protamine sulphate on ulcer healing, especially in the angiogenesis provoked by bFGF. 33,37 TNF-a was recently reported to have an angiogenic action. 43 The possibility that this was associated with the ulcer healing effect of protamine sulphate was also examined in the present study.…”

Section: Discussionmentioning

confidence: 99%

“…60 Furthermore, decrease in acid secretion would raise the gastric luminal pH which could stabilize bFGF and prolong its biological activity because endogenous bFGF is highly susceptible to continuous degradation and inactivation by gastric acid. 33,37 Indeed, it was demonstrated that a cimetidine-dependent acid inhibition was accompanied by a bFGF increase at ulcer site. 33 Taken together, it was concluded that protamine sulphate could accelerate gastric ulcer healing.…”

Section: Discussionmentioning

confidence: 99%

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The ulcer healing effect of protamine sulphate in rat stomach

1999

Aliment Pharmacol Ther

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INTRODUCTIONProtamine sulphate is known for its neutralizing action on the anticoagulation effect of heparin. Moreover, it was recently reported that protamine sulphate could stimulate the release of endothelium-derived relaxing factor in canine arteries, 1 and also nitric oxide production in porcine carotid artery endothelial cells. 2 It was also involved in the regulation of neuronal nitric oxide synthase (NOS) activity. 3 A protamine sulphateproduced systemic hypotension could be attenuated by nitric oxide inhibition. 4 All these re¯ect a close relationship between the protamine sulphate and nitric oxide and their action on the cardiovascular system. It was reported that nitric oxide is a pivotal factor for gastric ulcer healing. The effect of endogenous nitric oxide on the healing process of gastric ulcer was investigated by using L-arginine, a substrate for NOS, and L-N G -monomethylarginine (L-NMMA) or L-N G -nitroarginine (L-NNA), the inhibitors of NOS. The results indicated that L-arginine accelerated ulcer healing and increased gastric angiogenesis, whereas L-NMMA or L-NNA resulted in a delay in ulcer healing and a reduction in the number of capillaries. 5,6 Our preliminary study showed that heparin accelerated gastric ulcer healing in rats. 7 The drug however, prolonged bleeding time, which was detrimental to gastric ulcer formation and its healing. In order to abolish this side-effect, we used protamine sulphate to neutralize such action, so that it could promote the healing action of heparin. Interestingly, protamine sulphate not only completely neutralized the anticoagulation action of heparin, but also potentiated its ulcer SUMMARY Background: Protamine sulphate has been reported to stimulate nitric oxide production from blood vessels, which is a pivotal factor for gastric ulcer healing. Our preliminary study also showed that protamine sulphate potentiated the ulcer healing effect of heparin. Methods: Male SD rats with acetic acid-induced gastric ulcers were given protamine sulphate (40±80 mg/kg, s.c.) twice daily for 4 or 7 days. L-N G -nitroarginine methyl ester (L-NAME, 5 mg/kg), an inhibitor of nitric oxide synthase (NOS), was given s.c. prior to protamine sulphate (80 mg/kg) treatment. Ulcer healing, angiogenesis, mucosal histological changes, NOS activity and growth factors were determined.

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“…It was suggested that, the active HGF, produced in the stomach after injury, may stimulate the proliferation of gastric mucosal epithelial cells through increased number of HGF receptor. Basic fibroblast growth factor (bFGF) a potent stimulant of angiogenesis, and accelerates the healing of experimental gastric and duodenal ulcers in rats [52,53]. In ulcerated human gastric mucosa, immunoreactive bFGF is upregulated [54], thought additional data suggest that bFGF mRNA expression is an early event after mucosal injury [55].…”

Section: Growth Factorsmentioning

confidence: 99%

The Role of Capsaicin-Sensitive Afferent Nerves in Gastric Mucosal Protection Initiated Centrally or Peripherally under Experimental Conditions

Gyires¹

2014

Capsaicin - Sensitive Neural Afferentation and the Gastrointestinal Tract: From Bench to Bedside

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Accelerated healing of duodenal ulcers by oral administration of a mutein of basic fibroblast growth factor in rats

Cited by 139 publications

References 10 publications

Effects of alfalfa chaff on the gastric mucosa in adult horses

Effects of alfalfa chaff on the gastric mucosa in adult horses

The ulcer healing effect of protamine sulphate in rat stomach

The Role of Capsaicin-Sensitive Afferent Nerves in Gastric Mucosal Protection Initiated Centrally or Peripherally under Experimental Conditions

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