Accelerated Healing of Infected Diabetic Wounds by a Dual-Layered Adhesive Film Cored with Microsphere-Loaded Hydrogel Composite Dressing

Abstract

Diabetic wounds, a prevalent chronic disease, are associated with older age. The hyperglycemic microenvironment in diabetic wounds significantly reduces the immune system, inducing bacterial invasion. The coupling of tissue repair and antibacterial treatment is critical for infected diabetic ulcer regeneration. In this study, a dual-layered sodium alginate/carboxymethyl chitosan (SA/CMCS) adhesive film cored with an SA-bFGF microsphere-loaded small intestine submucosa (SIS) hydrogel composite dressing with a graphene oxide (GO)-based antisense transformation system was developed to promote infected diabetic wound healing and bacterial eradication. Initially, our injectable SIS-based hydrogel composite stimulated angiogenesis, collagen deposition, and immunoregulation in diabetic wound repair. The GO-based transformation system subsequently inhibited bacterial viability in infected wounds by post-transformation regulation. Meanwhile, the SA/ CMCS film provided stable adhesion covering the wound area to maintain a moist microenvironment, which promoted in situ tissue repair. Our findings provide a promising clinical translation strategy for promoting the healing of infected diabetic wounds.

Electrospray Nano-Micro Composite Sodium Alginate Microspheres with Shape-Adaptive, Antibacterial, and Angiogenic Abilities for Infected Wound Healing

“…Many studies have shown that bFGF promotes active wound repair by promoting the rapid proliferation of cells involved in wound repair, thus significantly shortening wound healing time. − Moreover, prolonged release of bFGF from wound dressings has been found to enhance wound healing . Thus, we measured the functions of the nano-micro composite SAMSs on the releasing kinetics of bFGF.…”

Hydrogel-based dressings designed to facilitate wound healing

“…Furthermore, the SA/CMCS film provided stable adhesion to the wound, creating a moist wound environment that facilitated effective wound recovery. 279 Wang et al developed an injectable multifunctional hydrogel called FEMI hydrogel to tackle multidrug-resistant (MDR) bacterial infections in diabetic wounds. The FEMI hydrogel was formulated by incorporating EPL and MnO 2 nanosheets with a “nanoknife-like” structure.…”

Nanozymes for the Therapeutic Treatment of Diabetic Foot Ulcers

“…Chronic abnormal glucose metabolism in DFU patients inhibits antioxidant expression, induces excessive oxidative stress, and exacerbates wound inflammation . The high-glucose environment of the wound also provides ample nutrients for bacteria, increasing the risk of bacterial infection and impeding the resolution of inflammation . At the same time, localized hyperglycemia inhibits the proliferation and migration of fibroblasts and epithelial cells, limiting the process of cell proliferation and remodeling .…”