Accelerated induction of <i>in vitro</i> apatite formation by parallel alignment of hydrothermally oxidized titanium substrates separated by sub-millimeter gaps

Hayakawa, Satoshi; Okamoto, Keigo; Yoshioka, Tomohiko

doi:10.1080/21870764.2019.1572690

Cited by 7 publications

(5 citation statements)

References 58 publications

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“…However, the ability to form apatite was determined to be high on material surfaces with high surface energy, which is in agreement with the results of this study [ 52 , 53 ]. Furthermore, Hayakawa et al confirmed that the apatite-forming ability of Hank’s solution correlated with bone-forming ability in animal experiments, which is also in agreement with our results [ 54 , 55 , 56 , 57 ].…”

Section: Discussionsupporting

confidence: 93%

Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium

Hayashi,

Takao,

Komasa

et al. 2023

IJMS

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In this study, we applied argon plasma treatment to titanium surfaces with nanostructures deposited by concentrated alkali treatment and investigated the effects on the surface of the material and the tissue surrounding an implant site. The results showed that the treatment with argon plasma removed carbon contaminants and increased the surface energy of the material while the nanoscale network structure deposited on the titanium surface remained in place. Reactive oxygen species reduced the oxidative stress of bone marrow cells on the treated titanium surface, creating a favorable environment for cell proliferation. Good results were observed in vitro evaluations using rat bone marrow cells. The group treated with argon plasma exhibited the highest apatite formation in experiments using simulated body fluids. The results of in vivo evaluation using rat femurs revealed that the treatment improved the amount of new bone formation around an implant. Thus, the results demonstrate that argon plasma treatment enhances the ability of nanostructured titanium surfaces to induce hard tissue differentiation and supports new bone formation around an implant site.

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Section: Discussionsupporting

confidence: 93%

Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium

Hayashi,

Takao,

Komasa

et al. 2023

IJMS

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“…The low cost and easy electrochemical production of titanium dioxide nanotubular coatings, possessing beneficial properties, i.e., high surface-area-to-volume ratio, strong oxidizing properties, chemical stability, non-toxicity, good mechanical properties, excellent corrosion resistance, and high biointegration activity, is an especially promising modification method [7,8,[13][14][15][16][17]. Due to the similar topography to natural bone, such modified surfaces promote direct contact with bone cells and the formation of apatite, which is the main component of bone tissue [8,18,19]. Homogeneous nanotubular, but also nanoporous and nanosponge-like coatings, on the surface of a titanium/titanium alloy implant can be easily and quickly produced by controlled electrochemical anodization procedure, which has been meticulously optimized and described in our earlier reports [20][21][22][23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells

et al. 2020

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In vitro biological research on a group of amorphous titania coatings of different nanoarchitectures (nanoporous, nanotubular, and nanosponge-like) produced on the surface of Ti6Al4V alloy samples have been carried out, aimed at assessing their ability to interact with adipose-derived mesenchymal stem cells (ADSCs) and affect their activity. The attention has been drawn to the influence of surface coating architecture and its physicochemical properties on the ADSCs proliferation. Moreover, in vitro co-cultures: (1) fibroblasts cell line L929/ADSCs and (2) osteoblasts cell line MG-63/ADSCs on nanoporous, nanotubular and nanosponge-like TiO2 coatings have been studied. This allowed for evaluating the impact of the surface properties, especially roughness and wettability, on the creation of the beneficial microenvironment for co-cultures and/or enhancing differentiation potential of stem cells. Obtained results showed that the nanoporous surface is favorable for ADSCs, has great biointegrative properties, and supports the growth of co-cultures with MG-63 osteoblasts and L929 fibroblasts. Additionally, the number of osteoblasts seeded and cultured with ADSCs on TNT5 surface raised after 72-h culture almost twice when compared with the unmodified scaffold and by 30% when compared with MG-63 cells growing alone. The alkaline phosphatase activity of MG-63 osteoblasts co-cultured with ADSCs increased, that indirectly confirmed our assumptions that TNT-modified scaffolds create the osteogenic niche and enhance osteogenic potential of ADSCs.

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“…After surface modification, the UG-MH coating demonstrated the smallest contact angle (9.1°) and greatest hydrophilicity. Most scholars have maintained that hydrophilic implants are highly bioactive and induce the adsorption of proteins on the implant surface, which facilitates the adhesion, spreading, proliferation, and differentiation of osteoblasts, thereby promoting early bone formation surrounding the implants. − Compared with microrough titanium implants alone, hydrophilic modification of microrough titanium implants distinct increased the extent of osteogenic differentiation . Numerous studies have verified that the surface hydrophilicity of an implant is highly related to its bioactivity.…”

Section: Resultsmentioning

confidence: 99%

A Novel Cytocompatibility Strengthening Strategy of Ultrafine-Grained Pure Titanium

Xu¹,

Li²,

Xu³

et al. 2019

ACS Appl. Mater. Interfaces

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Ultrafine-grained pure (UFG) titanium processed by equal channel angular pressing possesses mechanical properties comparable to those of Ti-6Al-4V and features more favorable friction resistance, biocompatibility, and corrosion resistance than does commercially pure (CP) titanium. Nevertheless, UFG titanium is still a bio-inert material with a lack of bone-inducing ability. Here, TiO2-hydroxyapatite (TiO2-HA) coatings were fabricated on CP titanium and UFG titanium through combining micro-arc oxidation and hydrothermal treatment together to improve their cytocompatibility. The results indicate that, compared with conventional coatings that use CP titanium as the substrate, such coatings formed on the UFG titanium possess additional hydrophilicity and in vitro cytocompatibility. The fantastic hierarchical structure of the UFG TiO2-HA coating (UG-MH coating), including microscale and nanoscale pores and short column-shaped and sheet-shaped HA grains with varying geometric shapes, excellent hydrophilicity, and high polar force, enhances the mutual effects between the osteoblasts and titanium implant since it provides an adequate microenvironment for the ingrowth of osteoblasts, inducing osteoblast adhesion, proliferation, and differentiation. The UG-MH coating has a synergistic effect due to its fantastic hydrophilic hierarchical structure and high polar force on the up-regulated expression of cytoskeletal actin proteins as well as osteocalcin, protein kinase C (PKC), nuclear factor of activated T-cells (NFAT), and Wnt5, enabling osteoblasts to differentiate via the Wnt calcium-dependent signaling pathway. This study highlights the idea that the modified UFG titanium will be more suitable than CP titanium in dental and orthopedic applications.

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Accelerated induction of in vitro apatite formation by parallel alignment of hydrothermally oxidized titanium substrates separated by sub-millimeter gaps

Abstract: Yoshioka (2019) Accelerated induction of invitro apatite formation by parallel alignment of hydrothermally oxidized titanium substrates separated by sub-millimeter gaps,

Cited by 7 publications

References 58 publications

Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium

Effects of Argon Gas Plasma Treatment on Biocompatibility of Nanostructured Titanium

In Vitro Studies on Nanoporous, Nanotubular and Nanosponge-Like Titania Coatings, with the Use of Adipose-Derived Stem Cells

A Novel Cytocompatibility Strengthening Strategy of Ultrafine-Grained Pure Titanium

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