Accelerated neural network formation in rat cerebral cortex cultures chronically disinhibited with picrotoxin

Huizen, F. van; Romijn, H.J.; Habets, A.M.M.C.; Hooff, Peter van den

doi:10.1016/0014-4886(87)90089-6

Cited by 42 publications

(20 citation statements)

References 11 publications

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“…The topology of the network can, however, not be predicted by the model as for this purpose a more detailed model of axons and dendrites would be required. However, neuronal development towards homeostasis substantially accelerates by increasing neuronal activities due to disinhibition by picrotoxin, a GABAergic synapse blocker [43]. Considering other transmitter studies, neuronal activity via increased glutamate release is likely to promote axonal outgrowth [44], [45] and therefore leads to a faster synapse formation and to an earlier maturation of the cell culture.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, this paper is in the tradition of a sequence of investigations [17], [40], [43], [45] that try to link cell culture activity and development to possible in vivo stages. Indications exist indeed that different activity states in cultures could be matched to in vivo states [61], but one needs to clearly state that culture and in vivo development also show clear differences.…”

Section: Discussionmentioning

confidence: 99%

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Self-Organized Criticality in Developing Neuronal Networks

et al. 2010

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Recently evidence has accumulated that many neural networks exhibit self-organized criticality. In this state, activity is similar across temporal scales and this is beneficial with respect to information flow. If subcritical, activity can die out, if supercritical epileptiform patterns may occur. Little is known about how developing networks will reach and stabilize criticality. Here we monitor the development between 13 and 95 days in vitro (DIV) of cortical cell cultures (n = 20) and find four different phases, related to their morphological maturation: An initial low-activity state (≈19 DIV) is followed by a supercritical (≈20 DIV) and then a subcritical one (≈36 DIV) until the network finally reaches stable criticality (≈58 DIV). Using network modeling and mathematical analysis we describe the dynamics of the emergent connectivity in such developing systems. Based on physiological observations, the synaptic development in the model is determined by the drive of the neurons to adjust their connectivity for reaching on average firing rate homeostasis. We predict a specific time course for the maturation of inhibition, with strong onset and delayed pruning, and that total synaptic connectivity should be strongly linked to the relative levels of excitation and inhibition. These results demonstrate that the interplay between activity and connectivity guides developing networks into criticality suggesting that this may be a generic and stable state of many networks in vivo and in vitro.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Self-Organized Criticality in Developing Neuronal Networks

et al. 2010

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“…Conversely, NMDA receptor activation in developing neocortex networks induces new pre-synaptic release sites along with enhancement of vesicle turnover (Walz et al, 2006) while spontaneous action potential-driven glutamatergic excitation speeds up the establishment of functional connections between pairs of neurons (Le Bé and Markram, 2006) in acute rodent neocortical slices isolated at 2 weeks after birth, i.e., in the middle of the most rapid phase of new synapse formation (Van Huizen et al, 1985. Neocortical synaptogenesis is also initially accelerated when SAP firing is chronically intensified by means of GABAergic disinhibition (a treatment which, additionally, advances the age in vitro at which 'exuberant' synapses begin to be pruned away: Van Huizen et al, 1987b).…”

Section: Spontaneous Bioelectric Activity As An Epigenetic Factormentioning

confidence: 98%

Physiological consequences of selective suppression of synaptic transmission in developing cerebral cortical networks in vitro: Differential effects on intrinsically generated bioelectric discharges in a living ‘model’ system for slow-wave sleep activity

Corner¹,

Baker²,

Pelt³

2008

Neuroscience & Biobehavioral Reviews

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“…The dendritic trees of neurons are the primary recipients of electrophysiological input, and as such, are in a position to be affected by alterations in activity. A number of can regulate dendritic morphology and spine density (Coleman and Riesen, 1968;Fifkova, 1968;Valverde, 197 1;Ryugo et al, 1975a,b,c;Juraska, 1982;van Huizen et al, 1985van Huizen et al, , 1987McMullen and Glaser, 1988;Bryan and Riesen, 1989;Muller et al, 1993). In the visual cortex, various studies employing uniocular deprivation (Fifkova, 1968;Rothblat and Schwartz, 1979), binocular deprivation (Globus and Scheibel, 1967;Valverde, 1967Valverde, , 1971Coleman and Riesen, 1968;Freire, 1978), and enucleation (Ryugo et al, 1975b;Heumann and Rabinowicz, 1982) have shown that decreased visual input to the cortex during critical periods of development 'can result in a corresponding decrease in dendritic spine density on pyramidal neurons.…”

Section: Introductionmentioning

confidence: 99%

Activity‐dependent regulation of dendritic spine density on cortical pyramidal neurons in organotypic slice cultures

1994

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In order to examine the effects of activity on spine production and/or maintenance in the cerebral cortex, we have compared the number of dendritic spines on pyramidal neurons in slices of P0 mouse somatosensory cortex maintained in organotypic slice cultures under conditions that altered basal levels of spontaneous electrical activity. Cultures chronically exposed to 100 microM picrotoxin (PTX) for 14 days exhibited significantly elevated levels of electrical activity when compared to neurons in control cultures. Pyramidal neurons raised in the presence of PTX showed significantly higher densities of dendritic spines on primary apical, secondary apical, and secondary basal dendrites when compared to control cultures. The PTX-induced increase in spine density was dose dependent and appeared to saturate at 100 microM. Cultures exhibiting little or no spontaneous activity, as a result of growth in a combination of PTX and tetrodotoxin (TTX), showed significantly fewer dendritic spines compared to cultures maintained in PTX alone. These results demonstrate that the density of spines on layers V and VI pyramidal neurons can be modulated by growth conditions that alter the levels of spontaneous electrical activity.

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Accelerated neural network formation in rat cerebral cortex cultures chronically disinhibited with picrotoxin

Cited by 42 publications

References 11 publications

Self-Organized Criticality in Developing Neuronal Networks

Self-Organized Criticality in Developing Neuronal Networks

Physiological consequences of selective suppression of synaptic transmission in developing cerebral cortical networks in vitro: Differential effects on intrinsically generated bioelectric discharges in a living ‘model’ system for slow-wave sleep activity

Activity‐dependent regulation of dendritic spine density on cortical pyramidal neurons in organotypic slice cultures

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