Accelerated Oral Healing by Angelica gigas Nakai from Hot Melt Extrusion Technology: An In Vitro Study

Abstract

Background and Objectives: In spite of the oral environment being healing-prone, its dynamic changes may affect wound healing. The purpose of this study was to assess the oral wound healing effect of Angelica gigas Nakai (AG) prepared by hot-melt extrusion. Materials and Methods: Human gingival fibroblast (HGF) cells were treated with AG or AG via hot-melt extrusion (AGH) for 24 h to determine the optimal concentration. For evaluating the anti-inflammatory effect of AG and AGH, a nitric oxide assay was performed under lipopolysaccharide (LPS) stimulation. The wound-healing effects of AG and AGH were evaluated using cell proliferation/migration assays and wound-healing marker expression through qRT-PCR. Results: Both AG and AGH showed no cytotoxicity on HGH cells. Regarding nitric oxide production, AGH significantly decreased LPS-induced nitric oxide production (p < 0.05). AGH showed a significantly positive result in the cell proliferation/cell migration assay compared with that in AG and the control. Regarding wound healing marker expression, AGH showed significantly greater VEGF and COL1α1 expression levels than those in the others (p < 0.05), whereas α-SMA expression was significantly different among the groups. Conclusions: Within the limits of this study, AGH accelerated oral wound healing in vitro.

Anti-Oral Microbial Effect of Ethanol Extract of Angelica gigas Nakai

“…The main components of AGN are coumarin, a lipid-soluble substance, and polysaccharide, a water-soluble substance 22) . Cumarin includes pyranocoumarins (decursin and decursinol algelate), umbelliferone, nodakenin, peucedanone, marmesin, demethylsuberosin and isoimperatorin 23) . Decursin and decursinol algelate are the main active components of the biological effects of AGN 23,24) .…”

“…Cumarin includes pyranocoumarins (decursin and decursinol algelate), umbelliferone, nodakenin, peucedanone, marmesin, demethylsuberosin and isoimperatorin 23) . Decursin and decursinol algelate are the main active components of the biological effects of AGN 23,24) . AGN root contains more than 6% of the active components, decursin, decursinol angelate and nodakenin, in particular, decursin and decursinol angelate are not found in Chinese and Japanese Angelica species 25) .…”