Accelerated production of α2,8- and α2,9-linked polysialic acid in recombinant Escherichia coli using high cell density cultivation

Abstract

Highlights An implemented high cell density cultivation process for biotechnological polysialic acid production is shown. Efficiency of polysialic acid production using E. coli K1 is significantly improved compared to state-of-the-art processes. Non-pathogenic E. coli BL21 is successfully engineered for production of α2,8- and α2,9-linked polySia. Differences of the α2,8- and α2,9-linked polysialic acid can be markedly visualized by the recorded NMR spectra.