Accelerated tissue regeneration through incorporation of basic fibroblast growth factor-impregnated gelatin microspheres into artificial dermis

Kawai, Katsuya; Suzuki, Shigehiko; Tabata, Yasuhiko; Ikada, Yoshito; Nishimura, Yoshihiko

doi:10.1016/s0142-9612(99)00207-0

Cited by 269 publications

(168 citation statements)

References 37 publications

Supporting

Mentioning

164

Contrasting

Unclassified

Order By: Relevance

“…Various natural and synthetic matrices have been investigated as carriers for bFGF. Natural materials include gelatin [31,32], collagen [33], fibrin [34], heparin [35] and alginate. [36] Synthetic matrices have been generated from poly(ethylene glycol)-g-poly(lactic acid) [37], poly(lactic acid) [38], poly(lactic-co-glycolic acid) [39,40], and methylidene malonate polymers.…”

Section: Discussionmentioning

confidence: 99%

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

View full text Add to dashboard Cite

Scaffolds that better approximate the mechanical properties of cardiovascular and other soft tissues might provide a more appropriate mechanical environment for tissue development or healing in vivo. An ability to induce local angiogenesis by controlled release of an angiogenic factor, such as basic fibroblast growth factor (bFGF), from a biodegradable scaffold with mechanical properties more closely approximating soft tissue could find application in a variety of settings. Toward this end biodegradable poly(ester urethane)urea (PEUU) scaffolds loaded with bFGF were fabricated by thermally induced phase separation. Scaffold morphology, mechanical properties, release kinetics, hydrolytic degradation and bioactivity of the released bFGF were assessed. The scaffolds had interconnected pores with porosities of 90% or greater and pore sizes ranging from 34-173 μm. Scaffolds had tensile strengths of 0.25-2.8 MPa and elongations at break of 81-443%. Incorporation of heparin into the scaffold increased the initial burst release of bFGF, while the initial bFGF loading content did not change release kinetics significantly. The released bFGF remained bioactive over 21 days as assessed by smooth muscle mitogenicity. Scaffolds loaded with bFGF showed slightly higher degradation rates than unloaded control scaffolds. Smooth muscle cells seeded into the scaffolds with bFGF showed higher cell densities than for control scaffolds after 7 days of culture. The bFGFreleasing PEUU scaffolds thus exhibited a combination of mechanical properties and bioactivity that might be attractive for use in cardiovascular and other soft tissue applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Biodegradable elastomeric scaffolds with basic fibroblast growth factor release

Guan

Stankus

Wagner

2007

Journal of Controlled Release

149

View full text Add to dashboard Cite

show abstract

“…To overcome such problems different crosslinking techniques can be used to minimise or prevent contraction such as dehydrothermal (DHT) [28,42,[48][49][50][51][52][53][54][55], UV light [42,50] and chemical crosslinking [42,54,56,57]. Chemical methods include the use of glutaraldehyde [54,[58][59][60], 1-Ethyl-3-3dimethyl aminopropyl carbodiimide (EDAC) [42,49,54,61,62] and genipin [57]. DHT treatment consists of the removal of water present in the scaffold polymeric chains under vacuum and with temperature.…”

Section: Introductionmentioning

confidence: 99%

Controlled release of transforming growth factor-β3 from cartilage-extra-cellular-matrix-derived scaffolds to promote chondrogenesis of human-joint-tissue-derived stem cells

et al. 2014

View full text Add to dashboard Cite

“…G is a protein derived from collagen, and it has been frequently applied in artificial skin, bone grafts, and scaffolds for tissue engineering (Esposito et al, 1996;Kawai et al, 2000;Zhao et al, 2002;Ito et al, 2003;Chang et al, 2003). Its wide use in the biomedical field is motivated by the presence of Arg-Gly-Asp (RGD)-like sequences that promote cell adhesion and migration (Shen et al, 2000).…”

Section: Introductionmentioning

confidence: 99%