This paper investigated the fuel effi ciency and ecological aspects of CNG (compressed natural gas) buses in urban public transport in Belgrade. For this study, CNG bus equipped with lean burn combustion and OCs (oxidation catalysts), and diesel bus with EGR (exhaust gas recirculation)-CRT (continuously regenerating technology) were tested on four urban public transport lines (55, 58, 74 and 94). Based on the recorded data regarding bus speed, acceleration, deceleration and mileage, for typical stop distance, driving cycle and its parameters is defi ned for urban public transport lines in Belgrade. Results of this study showed that under identical conditions on the same lines, average consumption of diesel bus was 12% higher than for tested CNG bus, but average energy consumption was 15.7% lower. Applying ''Student's t-test'' it has been determined that emission of NOX from tested CNG bus is lower than for diesel bus for 40%, CO2 for 21.6%, while emission of HC is higher more than ten times.