Acceleration of chondrogenic differentiation of human mesenchymal stem cells by sustained growth factor release in 3D graphene oxide incorporated hydrogels

Shen, He; Lin, Hang; Sun, Aaron X.; Song, Saijie; Wang, Bing; Yang, Yuanheng; Dai, Jianwu; Tuan, Rocky S.

doi:10.1016/j.actbio.2020.01.048

Cited by 68 publications

(48 citation statements)

References 41 publications

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“…Recently, GO has been performed to test the effect on tissue engineering and regenerated medicine elds. Shen [28] developed a GO incorporated PDLLA hybrid hydrogel for localized TGF-β3 delivery and sustained release. The scaffold supported TGF-β3 retention for up to 4 weeks and enhanced scaffold compressive stiffness because of GO.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Qin¹,

Bao²,

Zeng³

et al. 2020

Preprint

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Background: Application of platelet-rich plasma (PRP) can improve tendon-bone healing (TBH) after rotator cuff surgical repair. Graphene Oxide (GO) is a steady, controlled, and sustained carrier. The purpose of this study is to determine whether GO/PRP Composite Scaffold enhances the TBH after RC surgical repair in a rabbit model.Methods: A full-thickness tear of the supraspinatus tendon was created and repaired in 36 adult male New Zealand rabbits. They were divided into three groups: Control group, PRP group, and GO/PRP Composite Scaffold group (GO group). The effect of GO/PRP Composite Scaffold on TBH was assessed using histological and biomechanical evaluations at 8 and 12 weeks postoperatively.Results: Histological analysis showed that greater continuity, better orientation, and more density of collagen fiber were detected in the GO group than PRP and Control groups at 8 and 12 weeks, respectively. Results of biomechanical evaluations showed that the load to failure and stiffness of the GO group were statistically higher than those of PRP and Control groups at both 8 and 12 weeks (P<0.05). Compared with 8 weeks in the GO group, there was no significant difference in load to failure at 12 weeks (P>0.05), while the stiffness at 12 weeks was higher than that at 8 weeks (P<0.05). Conclusions: These results demonstrated that GO/PRP Composite Scaffold enhanced the TBH following rotator cuff surgical repair in a rabbit model. The GO may be an effective carrier for PRP into repair sites.

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Section: Discussionmentioning

confidence: 99%

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Qin¹,

Bao²,

Zeng³

et al. 2020

Preprint

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“…Besides primary chondrocytes, human stem cells also serve as a promising cell source to engineer 3D cartilage tissues [239,240]. In particular, the higher availability of stem cells like adult MSCs makes them preferable in engineering individual-specific cartilage tissues for regenerative medicine and OA studies.…”

Section: D Engineered Cartilage Tissuesmentioning

confidence: 99%

Pathogenesis of Osteoarthritis: Risk Factors, Regulatory Pathways in Chondrocytes, and Experimental Models

Alexander

et al. 2020

Biology

Self Cite

188

115

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As the most common chronic degenerative joint disease, osteoarthritis (OA) is the leading cause of pain and physical disability, affecting millions of people worldwide. Mainly characterized by articular cartilage degradation, osteophyte formation, subchondral bone remodeling, and synovial inflammation, OA is a heterogeneous disease that impacts all component tissues of the articular joint organ. Pathological changes, and thus symptoms, vary from person to person, underscoring the critical need of personalized therapies. However, there has only been limited progress towards the prevention and treatment of OA, and there are no approved effective disease-modifying osteoarthritis drugs (DMOADs). Conventional treatments, including non-steroidal anti-inflammatory drugs (NSAIDs) and physical therapy, are still the major remedies to manage the symptoms until the need for total joint replacement. In this review, we provide an update of the known OA risk factors and relevant mechanisms of action. In addition, given that the lack of biologically relevant models to recapitulate human OA pathogenesis represents one of the major roadblocks in developing DMOADs, we discuss current in vivo and in vitro experimental OA models, with special emphasis on recent development and application potential of human cell-derived microphysiological tissue chip platforms.

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“…Growth factors can be adsorbed onto the large surface area of graphene or GO in high amounts to direct stem cell differentiation. This approach was demonstrated for hydrogels based on GO and either collagen [ 111 ] or poly-D,L-lactic acid/polyethylene glycol [ 112 ], designed to repair the cartilage. Chondroitin sulfate in another biomolecule of interest to promote regeneration of this tissue, and to this end it was crosslinked with a chemically modified GO, to yield a hydrogel that effectively stimulated the deposition of a collagen matrix from mesenchymal stem cells [ 113 ].…”

Section: Recent Advancements On Hydrogels With Carbon Nanomaterials For Medicinementioning

confidence: 99%

Smart Hydrogels Meet Carbon Nanomaterials for New Frontiers in Medicine

2021

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Carbon nanomaterials include diverse structures and morphologies, such as fullerenes, nano-onions, nanodots, nanodiamonds, nanohorns, nanotubes, and graphene-based materials. They have attracted great interest in medicine for their high innovative potential, owing to their unique electronic and mechanical properties. In this review, we describe the most recent advancements in their inclusion in hydrogels to yield smart systems that can respond to a variety of stimuli. In particular, we focus on graphene and carbon nanotubes, for applications that span from sensing and wearable electronics to drug delivery and tissue engineering.

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Acceleration of chondrogenic differentiation of human mesenchymal stem cells by sustained growth factor release in 3D graphene oxide incorporated hydrogels

Cited by 68 publications

References 41 publications

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Enhancement of Rotator Cuff Tendon-bone Healing with Graphene Oxide/PRP Composite Scaffold in a Rabbit Model: A Histological and Biomechanical Study

Pathogenesis of Osteoarthritis: Risk Factors, Regulatory Pathways in Chondrocytes, and Experimental Models

Smart Hydrogels Meet Carbon Nanomaterials for New Frontiers in Medicine

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