Acceleration of Thrombin Formation by a Plasma Component

Fantl, P; Nance, Margaret H.

doi:10.1038/158708a0

Cited by 75 publications

(13 citation statements)

References 2 publications

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“…Even the physiologists were fractionating plasma and recombining the fractions in various ways which did, or did not, lead to coagulation of the mixture. It is of interest that those who used salt or acid fractionation generally concluded that there are two essential plasma factors, prothrombin and fibrinogen, whereas those who used differential absorption and elution (71,190,195) maintained that there were three. This sort of conflict has appeared a t every stage of the clotting mechanism investigated by fractionation methods.…”

Section: The Basis For Current Theories Of Coagulationmentioning

confidence: 99%

Enzymology and the Blood Clotting Mechanism

Esnouf¹,

Macfarlane²

1968

Advances in Enzymology - And Related Areas of Molecular Biology

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Section: The Basis For Current Theories Of Coagulationmentioning

confidence: 99%

Enzymology and the Blood Clotting Mechanism

Esnouf¹,

Macfarlane²

1968

Advances in Enzymology - And Related Areas of Molecular Biology

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“…The results obtained by this procedure are influenced by other factors in plasma or lymph, as has been indicated by several workers (Quick, 1943;Fantl & Nance, 1946;Owren, 1947).…”

mentioning

confidence: 68%

Coagulation in lymph

Fantl¹,

Nelson²

1953

The Journal of Physiology

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It is the purpose of this communication to present data concerning the initial stages of the clotting of body fluids. The role of thrombocytes in the formation or the supply of thromboplastin has not been decided. It is believed by some workers that they are essential, whilst others think that thrombocytes do not influence the clotting process in a specific manner. Coagulation factors are present in both blood and lymph. The main difference between the two fluids is of quantitative nature, except that thrombocytes are not normally present in lymph (Howell, 1914 (1913). In the case of blood the results differed from each other by not more than 2 min, and in the case of lymph by not more than 5 min. Citrated lymph was prepared by adding 1 vol. of 0-13 M-trisodium citrate to 4 vol. of lymph. Citrated blood was obtained by adding 9 vol. of blood to 1 vol. of the citrate solution. Protein determinations were carried out by a biuret technique (Robinson & Hogden, 1940).For the fibrinogen estimations the specimens were diluted ten times with 0.15 M-NaCl and fibrin was produced with a commercial bovine thrombin preparation. The fibrin clot was washed with 0-15 M-NaCl until soluble proteins were absent and then its protein content was estimated.Prothrombin values were obtained by the technique of Quick (1945). The results obtained by this procedure are influenced by other factors in plasma or lymph, as has been indicated by several

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“…The &dquo;one-stage&dquo; technic seems primarily to be a measure of the rapidity of fibrin formation, and it is not necessarily an index of the quantity of circulating prothrombin. Recently, several substances have been found in plasma and serum which markedly accelerate the rate of conversion of prothrombin to thrombin (54)(55)(56)(57). These accelerator factors form an important link in the autocatalytic process of clotting, since they themselves are activated by thrombin and then are able to accelerate the further conversion of prothrombin to thrombin.…”

Section: Natural Anticoagulantsmentioning

confidence: 99%