Acceleration of wound healing in acute full-thickness skin wounds using a collagen-binding peptide with an affinity for MSCs

Abstract

Mesenchymal stem cells (MSCs) have been accepted as a promising cell source in tissue repair and regeneration. However, the inability to enrich MSCs in target areas limits their wide application. As a result, it has been a major goal to induce MSCs to be abundantly and specifically recruited to the injury site. In this study, a peptide with a specific affinity for MSCs (E7 peptide) was immobilized to a collagen scaffold via a collagen-binding domain (CBD) to construct a functional collagen scaffold. In addition, the hypothesis that this method could recruit MSCs specifically was evaluated in a porcine model. In vivo investigations indicated that due to the immunore-action, the CBD-MSC-peptide collagen scaffold enhanced MSC adhesion and infiltration and promoted wound healing. At day 7 after surgery, we found more infiltrating cells and capillaries in the Collagen/CBD-E7 peptide group compared to the Scaffold group. At day 14, 21 and 28, a faster healing process was observed in the Collagen/CBD-E7 peptide group, with significant differences compared with the other groups (P < 0.05, P < 0.01). The results demonstrate the potential use of targeted therapy to rapidly heal skin wounds.

Comparison of a Fully Synthetic Electrospun Matrix to a Bi-Layered Xenograft in Healing Full Thickness Cutaneous Wounds in a Porcine Model

“…By Day 30, two of the three treatment group wounds had a wound area of 0 cm 2 , indicating complete healing. Such extensive wound surface area reduction in the treatment group compares well to trends observed by Wang, et al when characterizing a novel collagen-peptide bound synthetic scaffold in a full-thickness porcine model [ 12 ]. At 28 days, Wang, et al concluded that wounds treated with the novel bioactive scaffold exhibited a 96.9% reduction in size.…”

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

“…The presence of the peptide increased the efficiency of stem cell recruitment into polycaprolactone (PCL) meshes both in vitro and in vivo in a rat model in contrast to RGD peptide (Figure 1C-E). Similarly, the E7 peptide was recently combined with a collagen-binding domain and was able to enhance mesenchymal stem cell adhesion and infiltration in the collagen scaffolds in vivo in a porcine model [45] .…”

Progress in development of bioderived materials for dermal wound healing

“…Intensity of inflammation was significantly lower in FG group than in control group at day 7.[71]CollagenIncorporation with gold nanoparticlesCytotoxicity assay: L929 cells incubated in CS–AuX extract for 24 h; MTT cell viability assay: cell viability for all scaffold extracts was higher than 90%. Cell attachment assay: L929 fibroblasts seeded on CS–AuX for 24 h; SEM: fibroblasts on scaffolds gained their natural spindle-like shape with outstretched pseudopods spreading over the surface.CS–AuX on artificial wounds in live rat model for 14 days: milder inflammatory reaction and higher neovascularization were observed with CS–AuX than in other groups; better wound closure was observed with CS–X, CS–AuX and MatriDerm than in untreated control.[72]Modification with CBD-E7 peptide—Collagen/CBD-E7 peptide on artificial wounds in live porcine model for 28 days: significantly more MSCs were retained on CBD-E7 collagen scaffold than on pristine collagen scaffold at day 3 post-surgery; significantly rapid wound healing in collagen/CBD-E7 peptide group at days 14, 21 and 28 than in other groups; significantly higher capillary density in collagen/CBD-E7 peptide group than in other groups.[73]SSD, C 10 H 9 AgN 4 O 2 S; NHEK, normal human epidermal keratinocyte; NHEF, normal human epidermal fibroblast; HaCaT, human keratinocytes; LPS, lipopolysaccharides; Raw264.7, murine monocytes; CS–AuX, collagen-containing gold nanoparticles; MSCs, mesenchymal stem cells.…”