Acceptor specificity of cellobiose phosphorylase from Cellvibrio gilvus: synthesis of three branched trisaccharides

Percy, Ann; Ono, Hiroshi; Hayashi, Kiyoshi

doi:10.1016/s0008-6215(98)00109-8

Cited by 38 publications

(17 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The effects of temperature are particularly dramatic. A comparison of maximum growth rate across cellulolytic species reveals a 526 LYND ET AL. MICROBIOL.…”

Section: Rate-limiting Factors In Naturementioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,045

2,868

View full text Add to dashboard Cite

Fundamental features of microbial cellulose utilization are examined at successively higher levels of aggregation encompassing the structure and composition of cellulosic biomass, taxonomic diversity, cellulase enzyme systems, molecular biology of cellulase enzymes, physiology of cellulolytic microorganisms, ecological aspects of cellulase-degrading communities, and rate-limiting factors in nature. The methodological basis for studying microbial cellulose utilization is considered relative to quantification of cells and enzymes in the presence of solid substrates as well as apparatus and analysis for cellulose-grown continuous cultures. Quantitative description of cellulose hydrolysis is addressed with respect to adsorption of cellulase enzymes, rates of enzymatic hydrolysis, bioenergetics of microbial cellulose utilization, kinetics of microbial cellulose utilization, and contrasting features compared to soluble substrate kinetics. A biological perspective on processing cellulosic biomass is presented, including features of pretreated substrates and alternative process configurations. Organism development is considered for “consolidated bioprocessing” (CBP), in which the production of cellulolytic enzymes, hydrolysis of biomass, and fermentation of resulting sugars to desired products occur in one step. Two organism development strategies for CBP are examined: (i) improve product yield and tolerance in microorganisms able to utilize cellulose, or (ii) express a heterologous system for cellulose hydrolysis and utilization in microorganisms that exhibit high product yield and tolerance. A concluding discussion identifies unresolved issues pertaining to microbial cellulose utilization, suggests approaches by which such issues might be resolved, and contrasts a microbially oriented cellulose hydrolysis paradigm to the more conventional enzymatically oriented paradigm in both fundamental and applied contexts

show abstract

“…The effects of temperature are particularly dramatic. A comparison of maximum growth rate across cellulolytic species reveals a 526 LYND ET AL. MICROBIOL.…”

Section: Rate-limiting Factors In Naturementioning

confidence: 99%

Microbial Cellulose Utilization: Fundamentals and Biotechnology

Lynd

Weimer

Zyl³

et al. 2002

Microbiol Mol Biol Rev

4,045

2,868

View full text Add to dashboard Cite

show abstract

“…This compared with a shift of 0.55 ppm for the equivalent proton in 4-nitrophenol-b-D D -cellobioside and 0.44 (Percy et al, 1998). The free 6 A and 6 B (methylene) protons of residue II were more shielded (by about 0.4 and 0.7 ppm) than the free 6-protons in residue III and in b- (Percy et al, 1998). This observation suggested that the conformation of compound 5 in solution had the 6-protons of residue III close, and perpendicular, to one aromatic ring of the BPA moiety.…”

Section: Resultsmentioning

confidence: 82%

“…Thus a downfield shift, compared with glucose, of 0.54 ppm was seen for the 4-proton. This compared with a shift of 0.55 ppm for the equivalent proton in 4-nitrophenol-b-D D -cellobioside and 0.44 (Percy et al, 1998). The free 6 A and 6 B (methylene) protons of residue II were more shielded (by about 0.4 and 0.7 ppm) than the free 6-protons in residue III and in b- (Percy et al, 1998).…”

Section: Resultsmentioning

confidence: 87%

Glycosylation of bisphenol A by tobacco BY-2 cells

Nakajima¹,

Oshima²,

Edmonds³

et al. 2004

Phytochemistry

View full text Add to dashboard Cite

“…10 In the reverse reaction, CBP catalyzes the production of heterodisaccharides from various derivatives of glucose. [11][12][13][14][15][16][17][18][19] CBP was expected to react with D D-glucal as a hydrolytic enzyme, because the reaction mechanism of CBP is considered to share the identical reaction mechanism with inverting hydrolytic enzymes, as evidenced by 3D structural analysis of chitobiose phosphorylase that possesses high sequence similarity with CBP. 20 If CBP were to utilize D D-glucal as a glycosyl donor, it would be an excellent catalyst for the synthesis of the b-(1!4) linkage of 2-deoxy-D D-arabino-hexopyranose.…”

mentioning

confidence: 99%