Access to exercise and its relation to cardiovascular health and gene expression in laboratory animals

Schweitzer, Natalie B.; Alessio, Helaine M.; Hagerman, Ann; Roy, Sashwati; Sen, Chandan K.; Nagy, Szilvia; Byrnes, R; Philip, Ben; Woodward, Jane; Wiley, R. L.

doi:10.1016/j.lfs.2005.01.036

Cited by 10 publications

(6 citation statements)

References 31 publications

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“…We assessed transcriptomic changes in 7EX hearts to identify the breadth of molecular adaptations. Prior studies assess transcriptomic responses to more intense or prolonged exercise (5,46,48), with considerable variability in effects on the cardiac transcriptome. Here, only a small subset of transcripts (142 known genes) was sensitive to voluntary running (in keeping with low intensity of the activity), and changes were modestonly Ifi27l2 changed more than twofold ( Table 4).…”

Section: Discussionmentioning

confidence: 99%

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Voluntary running in mice beneficially modulates myocardial ischemic tolerance, signaling kinases, and gene expression patterns

Budiono

Hoe

Peart

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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Exercise triggers hormesis, conditioning hearts against damaging consequences of subsequent ischemia-reperfusion (I/R). We test whether "low-stress" voluntary activity modifies I/R tolerance and molecular determinants of cardiac survival. Male C57BL/6 mice were provided 7-day access to locked (7SED) or rotating (7EX) running-wheels before analysis of cardiac prosurvival (Akt, ERK 1/2) and prodeath (GSK3β) kinases, transcriptomic adaptations, and functional tolerance of isolated hearts to 25-min ischemia/45-min reperfusion. Over 7 days, 7EX mice increased running from 2.1 ± 0.2 to 5.3 ± 0.3 km/day (mean speed 38 ± 2 m/min), with activity improving myocardial I/R tolerance: 7SED hearts recovered 43 ± 3% of ventricular force with diastolic contracture of 33 ± 3 mmHg, whereas 7EX hearts recovered 63 ± 5% of force with diastolic dysfunction reduced to 23 ± 2 mmHg (P < 0.05). Cytosolic expression (total protein) of Akt and GSK3β was unaltered, while ERK 1/2 increased 30% in 7EX vs. 7SED hearts. Phosphorylation of Akt and ERK 1/2 was unaltered, whereas GSK3β phosphorylation increased ∼90%. Microarray interrogation identified significant changes (≥1.3-fold expression change, ≤5% FDR) in 142 known genes, the majority (92%) repressed. Significantly modified paths/networks related to inflammatory/immune function (particularly interferon-dependent), together with cell movement, growth, and death. Of only 14 induced transcripts, 3 encoded interrelated sarcomeric proteins titin, α-actinin, and myomesin-2, while transcripts for protective actin-stabilizing ND1-L and activator of mitochondrial biogenesis ALAS1 were also induced. There was no transcriptional evidence of oxidative heat-shock or other canonical "stress" responses. These data demonstrate that relatively brief voluntary activity substantially improves cardiac ischemic tolerance, an effect independent of shifts in Akt, but associated with increased total ERK 1/2 and phospho-inhibition of GSK3β. Transcriptomic data implicate inflammatory/immune and sarcomeric modulation in activity-dependent protection.

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Section: Discussionmentioning

confidence: 99%

“…Relatively few studies assess cardiac effects of voluntary exercise (11,14,23,40,46), and fewer still address brief voluntary activity (27). In the current study, we examine the efficacy of 7 days of voluntary wheel-running, a relatively low-stress form of activity (26,37,40), in improving cardiac ischemic tolerance in healthy male mice.…”

mentioning

confidence: 99%

Voluntary running in mice beneficially modulates myocardial ischemic tolerance, signaling kinases, and gene expression patterns

Budiono

Hoe

Peart

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

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“…Bronikowski et al (2003) reported a quantitative gene expression analysis in mice exposed to active life style over 16 generations. Schweizer et al (2005) measured the changes in gene expression in rats exercising each-other-day with a running wheel. Other studies showed that low-intensity (55-60% of VO 2max ) exercise training may not allow a suYcient cardioprotection (Starnes et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Mild exercise training, cardioprotection and stress genes profile

et al. 2007

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To improve current knowledge of the molecular mechanisms underlying exercise-induced cardioprotection in a rat model of mild exercise training, Sprague-Dawley rats were trained to run on a treadmill up to 55% of their maximal oxygen uptake for 1 h/day, 3 days/week, 14 weeks, with age-matched sedentary controls (n = 20/group). Rats were sacriWced 48 h after the last training session. Despite lack of cardiac hypertrophy, training decreased blood hemoglobin (7.94 § 0.21 mM vs. 8.78 § 0.23 mM, mean § SE, P = 0.01) and increased both plasma malondialdehyde (0.139 § 0.005 mM vs. 0.085 § 0.009 mM, P = 0.05) and the activity of Mn-superoxide dismutase (11.6 § 0.6 vs. 16.5 § 1.6 mU/ g, P = 0.01), whereas total superoxide dismutase activity was unaVected. When subjected to 30-min ischemia followed by 90-min reperfusion, hearts from trained rats (n = 5) displayed reduced infarct size as compared to controls (37.26 § 0.92% vs. 49.09 § 2.11% of risk area, P = 0.04). The biochemical analyses in the myocardium, which included gene expression proWles, realtime PCR, Western blot and determination of enzymatic activity, showed training-induced upregulation of the following mRNAs and/or proteins: growtharrest and DNA-damage induced 153 (GADD153/ CHOP), heme-oxygenase-1 (HO-1), cyclooxygenase-2 (Cox-2), heat-shock protein 70/72 (HSP70/72), whereas heat-shock protein 60 (HSP60) and glucoseregulated protein 75 (GRP75) were decreased. As a whole, these data indicate that mild exercise training activates a second window of myocardial protection against ischemia/reperfusion by upregulating a number of protective genes, thereby warranting further investigation in man.

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“…Exercise training up to 12 weeks of voluntary exercise has no significant effect on intestine ABCA1 gene expression, as well as, has no significant effect on liver and intestine ABCG5 and ABCG8 gene expression [47]. Longer duration training protocols appear to have more significant effects than shorter protocols in that 60 weeks of voluntary exercise reduces heart ABCA1 expression [41].…”

Section: Discussionmentioning

confidence: 99%

“…Schweitzer et al (2005) showed that aerobic exercise reduces heart ABCA1 expression [41]. Ghanbari-Niaki et al (2007) showed that endurance exercise enhances ABCA1 gene expression in rat liver [42].…”

Section: Abca1mentioning

confidence: 99%

Effects of exercise on reverse cholesterol transport: A systemized narrative review of animal studies

et al. 2019

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Aims: Reverse Cholesterol Transport (RCTr) is the mechanism by which excess cholesterol from peripheral tissues is transported to the liver for hepatobiliary excretion, thereby inhibiting foam cell formation and the development of atherosclerosis. Exercise affects RCTr, by influencing high-density lipoprotein cholesterol (HDL) through remodeling and by promoting hepatobiliary sterol excretion. The objectives of this systematized review of animal studies is to summarize the literature and provide an overview of the effects of chronic exercise (at least two weeks) on apoliproteins (Apo A-I, Apo-E), Paraoxonase-1 (PON1), ATP-binding cassette transporters (ABCA1, ABCG1, ABCG4, ABCG5, ABCG8), scavenger receptor class B type I (SR-BI), cholesteryl ester transfer protein (CETP), lowdensity lipoprotein receptor (LDLr) and cholesterol 7 alpha-hydroxylase (CYP7A1) and Niemann-Pick C1-like 1 (NPC1L1).

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Access to exercise and its relation to cardiovascular health and gene expression in laboratory animals

Cited by 10 publications

References 31 publications

Voluntary running in mice beneficially modulates myocardial ischemic tolerance, signaling kinases, and gene expression patterns

Voluntary running in mice beneficially modulates myocardial ischemic tolerance, signaling kinases, and gene expression patterns

Mild exercise training, cardioprotection and stress genes profile

Effects of exercise on reverse cholesterol transport: A systemized narrative review of animal studies

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