United Nations’ Agenda 2030 highlights the need to “make cities and human settlements inclusive, safe, resilient and sustainable” (SDG 11) and reduce inequalities (SDG 10) for the good health and well-being (SDG 3) of the citizens. Although most Italian museums have been forced to comply with the rules for accessibility in public spaces since 1971 following Law 30/3/1971 n. 118 and, more recently, Legislative Decree 81/2008, in this context, Universal Design can contribute to the achievement of these goals by improving accessibility to spaces, services, and the community’s well-being on the one hand, along with providing a deeper comprehension and awareness of the informational processes on the other hand. In this regard, museum organizations play a critical role in enhancing the quality of life and society’s education by preserving and distributing cultural heritage over time. Despite the large number of studies focused on museum organizations, there is little research that takes into account Universal Design and its macro-and firm-level economic benefits on museum institutions (not-for-profit, public, and private). Considering this research gap, this study’s aim is twofold: 1) to explore how some Italian museums have actually improved their facilities and services according to Universal Design principles and 2) to assess the economic museum accessibility. A qualitative methodology was applied using a questionnaire delivered to eight Italian museum, and collected data was compared. The findings underline how museums’ investments in Universal Design may produce relevant economic benefits, both on the macroeconomic and firm levels, providing guidelines for public policies concerning welfare, transport, environment, education, and well-being. Universal Design principles can lead museums to an increasing convergence toward Sustainable Development Goals, improving their overall economic performance as well as strengthening their role in a more aware and participative society. This study has several relevant implications in terms of both policy and management. Policymakers should promote Universal Design investments in museums in order to ensure accessibility to a greater number of visitors, and cultural institutions should consider accessibility as a specific key management dimension to be monitored and improved.