Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Hóng, Ní; Li, Mingyou; Zeng, Zhiqiang; Yi, Meisheng; Deng, Jiaorong; Gui, Jian‐Fang; Winkler, Christoph; Schartl, Manfred; Hong, Yunhan

doi:10.1007/s00018-009-0247-4

Cited by 34 publications

(67 citation statements)

References 36 publications

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“…29,30 Labeled ZES1 and MES1 cells were suspended in transplantation medium (TM; 100 mM NaCl, 5 mM KCl, 5 mM HEPES, pH 7.1). Zebrafish embryos were treated for 2 min with pronase E (1 mg/mL; Sigma) at 26°C for dechorionation.…”

Section: Embryonic Stem Cell Feeder-free Pluripotencymentioning

confidence: 99%

Derivation and Long-Term Culture of an Embryonic Stem Cell-Like Line from Zebrafish Blastomeres Under Feeder-Free Condition

Goh

Gan

et al. 2014

Zebrafish

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Existing zebrafish embryonic stem (ES) cell lines are derived and maintained using feeder layers. We describe here the derivation and long-term culture of an ES cell-like line derived from zebrafish blastomeres without the use of feeder cells. This line, designated as ZES1, has been maintained for more than 800 days in defined Dulbecco's modified Eagle's medium supplemented with fetal bovine serum, zebrafish embryo extract, trout serum, and human basic fibroblast growth factor. ZES1 cells possessed a morphology typical of ES cells, being round or polygonal in shape with a large nucleus and sparse cytoplasm and were mostly diploid. The cells formed individual colonies consisting of tightly packed cells that stained positively for alkaline phosphatase. ZES1 cells also formed embryoid bodies when transferred onto uncoated wells. The pluripotent nature of ZES1 cells was confirmed when they could be induced to differentiate in vitro into several cell types, through low-or high-density culture conditions. Treatment with retinoic acid also induced the differentiation of ZES1 cells into primarily neuronal cells. Using immunostaining and real-time polymerase chain reaction, we showed that Sox2, a known pluripotent marker in mammalian ES cells, was also present in ZES1 cells. Chimera experiments revealed that fluorescent-labeled ZES1 cells microinjected into zebrafish blastulas participated in the formation of all three germ layers. Using GFP-labeled ZES1 cells, chimera germline transmission was also demonstrated at the F 1 generation. In conclusion, ZES1 cells possess both in vitro and in vivo pluripotency characteristics, indicating that nonmammalian ES cells can be readily derived and maintained for a long term under feeder-free culture conditions.

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Section: Embryonic Stem Cell Feeder-free Pluripotencymentioning

confidence: 99%

Derivation and Long-Term Culture of an Embryonic Stem Cell-Like Line from Zebrafish Blastomeres Under Feeder-Free Condition

Goh

Gan

et al. 2014

Zebrafish

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“…Of the medaka albino host strains, only strain i 1 gives rise to pigmented chimeras from MES1 cells. Strikingly, this accessibility to ES cells was completely lost in i 1 but acquired in strain i 3 after host embryos were gamma-irradiated [23]. This observation provides an alternative clue to adjust the donorhost compatibility in chimera formation.…”

Section: Chimera Formation From Medaka Es Cellsmentioning

confidence: 82%

Medaka fish stem cells and their applications

Hóng

et al. 2010

Sci. China Life Sci.

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Stem cells are present in developing embryos and adult tissues of multicellular organisms. Owing to their unique features, stem cells provide excellent opportunities for experimental analyses of basic developmental processes such as pluripotency control and cell fate decision and for regenerative medicine by stem cell-based therapy. Stem cell cultures have been best studied in 3 vertebrate organisms. These are the mouse, human and a small laboratory fish called medaka. Specifically, medaka has given rise to the first embryonic stem (ES) cells besides the mouse, the first adult testis-derived male stem cells spermatogonia capable of test-tube sperm production, and most recently, even haploid ES cells capable of producing Holly, a semi-cloned fertile female medaka from a mosaic oocyte created by microinjecting a haploid ES cell nucleus directly into a normal oocyte. These breakthroughs make medaka a favoring vertebrate model for stem cell research, the topic of this review.

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“…Medaka (Oryzias latipes) strain i 1 and transgenic line Vg were maintained under an artificial photoperiod of 14-h/10-h light/darkness at 26°C as described [40,45,46]. In medaka, sex maturation occurs until 3 months post hatching.…”

Section: Fishmentioning

confidence: 99%

“…Testes were fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) at 4°C for 1 h and subjected to cryosectioning as described [46]. The sections were stained for nuclei with DAPI (1 µg/ml) for 10 min at room temperature, washed three times in PBS and mounted for microscopy [55].…”

Section: Testicular Cryosectionsmentioning

confidence: 99%

Retinoic Acid Regulates Germ Gene Transcription In Vitro and Spermatogenesis in Testicular Organ Culture

Hóng¹,

Li²

2012

Biol Syst Open Access

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The adult testis is the male reproductive organ that produces sex hormones for sexual behaviours and sperm in a well-controlled process called spermatogenesis. Spermatogenesis involves self-renewal and differentiation of the male germ stem cells spermatogonia, culminating in the production of sperm for germline transmission. Retinoic acid (RA) is essential for fate decision and meiosis entry of mammalian germ cells. The microphthalmia-associated transcription factor (Mitf) is a newly identified transcriptional activator of germ genes in the fish medaka (Oryzias latipes). Here we report an essential role of RA on Mitf-activated germ gene expression and spermatogenesis. Medaka germ gene promoters DAZ, DND and VAS were found to contain RA-responsive elements and to exhibit little Mitf-activated transcriptional activity in response to RA treatment in both medaka ES cells and male germ stem cells. A procedure for testicular organ culture was established, which allows for test-tube spermatogenesis over a 21-day period. By the ordered testicular architecture, cellular morphology and VAS-driven GFP expression, different spermatogenic stages were identifiable on testicular cross-sections. In organ culture, RA treatment generated testes completely free of spermatogonia, and reduced VAS-driven GFP expression in spermatogonia but enhanced its expression in meiotic and post-meiotic germ cells. Therefore, RA represses Mitf-activated germ gene expression in stem cell cultures and differentially regulates germ gene expression depending upon the stages of spermatogenesis, and represses spermatogonial stem cell maintenance in cultured testes of medaka as a lower vertebrate model. These results demonstrate that RA plays a highly conserved role in germ cell development from fish to mammals.

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Accessibility of host cell lineages to medaka stem cells depends on genetic background and irradiation of recipient embryos

Cited by 34 publications

References 36 publications

Derivation and Long-Term Culture of an Embryonic Stem Cell-Like Line from Zebrafish Blastomeres Under Feeder-Free Condition

Derivation and Long-Term Culture of an Embryonic Stem Cell-Like Line from Zebrafish Blastomeres Under Feeder-Free Condition

Medaka fish stem cells and their applications

Retinoic Acid Regulates Germ Gene Transcription In Vitro and Spermatogenesis in Testicular Organ Culture

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