2024
DOI: 10.1371/journal.pone.0301079
|View full text |Cite
|
Sign up to set email alerts
|

Accessible luminal interface of bovine rectal organoids generated from cryopreserved biopsy tissues

Minae Kawasaki,
Yoko M. Ambrosini

Abstract: Developing precise species-specific in vitro models that closely resemble in vivo intestinal tissues is essential for advancing our understanding of gastrointestinal physiology and associated diseases. This is especially crucial in examining host-pathogen interactions, particularly in bovines, a known reservoir for microbes and pathogens posing substantial public health threats. This research investigated the viability of producing bovine rectal organoids from cryopreserved tissues. We compared two cryopreserv… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

1
2
0

Year Published

2024
2024
2024
2024

Publication Types

Select...
2

Relationship

1
1

Authors

Journals

citations
Cited by 2 publications
(3 citation statements)
references
References 53 publications
1
2
0
Order By: Relevance
“…It has also been proposed that expression of genes or cell surface receptors which influence bacterial invasion or host immune response could have been altered in these models 8 , 45 . These potential explanations are also applicable to the organoid-derived monolayer systems due to their physiological resemblance to in vivo tissue attributable to their multi-cellular nature as demonstrated by the present and several other studies 11 , 17 , 46 .…”
Section: Discussionsupporting
confidence: 63%
See 2 more Smart Citations
“…It has also been proposed that expression of genes or cell surface receptors which influence bacterial invasion or host immune response could have been altered in these models 8 , 45 . These potential explanations are also applicable to the organoid-derived monolayer systems due to their physiological resemblance to in vivo tissue attributable to their multi-cellular nature as demonstrated by the present and several other studies 11 , 17 , 46 .…”
Section: Discussionsupporting
confidence: 63%
“…The epithelial barrier integrity of the monolayers was evaluated by performing permeability assay on Days 1, 3 and 5 of culture following the previously described protocol 46 , 55 . Briefly, 0.5 mg/mL of 4 kDa FITC-dextran was applied to the apical compartment at time 0, and the fluorescence intensity of the culture medium in the basolateral compartment was determined every 20 min over 120 min using SpectraMax i3x microplate reader (molecular devices).…”
Section: Methodsmentioning
confidence: 99%
See 1 more Smart Citation