Accession-dependent action potentials in Arabidopsis

Favre, Patrick; Greppin, Hubert; Agosti, Robert Degli

doi:10.1016/j.jplph.2010.09.014

Cited by 14 publications

(12 citation statements)

References 56 publications

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“…The physiological meaning of Ca 2+ waves observed within vascular tissues (but not solely there) is one of the interesting aspects which could be investigated. Velocity values observed for these Ca 2+ signals are of the same order of magnitude than those of “fast” electrical signals (i.e., “action potentials”) reported to travel leaf tissues in Arabidopsis (Favre et al, 2011). This substantiates the hypothesis of an interplay between Ca 2+ and electrical signaling (Król et al, 2011).…”

Section: Discussionmentioning

confidence: 67%

Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter

Xiong¹,

Ronzier²,

Sanchez³

et al. 2014

Front. Plant Sci.

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Calcium (Ca2+) is a second messenger involved in many plant signaling processes. Biotic and abiotic stimuli induce Ca2+ signals within plant cells, which, when decoded, enable these cells to adapt in response to environmental stresses. Multiple examples of Ca2+ signals from plants containing the fluorescent yellow cameleon sensor (YC) have contributed to the definition of the Ca2+ signature in some cell types such as root hairs, pollen tubes and guard cells. YC is, however, of limited use in highly autofluorescent plant tissues, in particular mesophyll cells. Alternatively, the bioluminescent reporter aequorin enables Ca2+ imaging in the whole plant, including mesophyll cells, but this requires specific devices capable of detecting the low amounts of emitted light. Another type of Ca2+ sensor, referred to as GFP-aequorin (G5A), has been engineered as a chimeric protein, which combines the two photoactive proteins from the jellyfish Aequorea victoria, the green fluorescent protein (GFP) and the bioluminescent protein aequorin. The Ca2+-dependent light-emitting property of G5A is based on a bioluminescence resonance energy transfer (BRET) between aequorin and GFP. G5A has been used for over 10 years for enhanced in vivo detection of Ca2+ signals in animal tissues. Here, we apply G5A in Arabidopsis and show that G5A greatly improves the imaging of Ca2+ dynamics in intact plants. We describe a simple method to image Ca2+ signals in autofluorescent leaves of plants with a cooled charge-coupled device (cooled CCD) camera. We present data demonstrating how plants expressing the G5A probe can be powerful tools for imaging of Ca2+ signals. It is shown that Ca2+ signals propagating over long distances can be visualized in intact plant leaves and are visible mainly in the veins.

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Section: Discussionmentioning

confidence: 67%

Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter

Xiong¹,

Ronzier²,

Sanchez³

et al. 2014

Front. Plant Sci.

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“…The abiotic or biotic stimulus including drought, salt stimulation, cold stimulation, diseases and insect pests etc. can trigger various electrical activities of plants [10,11,22,23]. Meanwhile, these stimulu can also cause changes of intracellular water status in plant leaves.…”

Section: Discussionmentioning

confidence: 99%

New sight: rapid monitor of intracellular water information in plant leaves based on electrophysiological parameters

Zhang

Su³

et al. 2020

Preprint

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Background: Intracellular water in plant leaves is paramount importance to physiological and biochemical processes. Water changes trigger the rapid response of plant electrophysiological information. In this study, plant electrophysiological information was firstly used to rapid monitor intracellular water information in plant leaves. Results: Based on the thermodynamics laws, the fitting equations between plant electrophysiological parameters and clamping force were innovative established. Subsequently, leaf intrinsic electrophysiological parameters, intracellular water-holding capacity (IWHC), water use efficiency (IWUE), water-holding time (IWHT) and water transfer rate (WTR), were firstly developed and applied. The results show that the leaves of Broussonetia papyrifera in the cultivated soil had higher IWHC, WTR and water content than in the moderate rocky desertification soil. The leaf IWHC, WTR and water content of the herbaceous plants were higher than that of the woody plants. Solanum tuberosum had higher leaf IWHC, WTR and lower IWUE, IWHT, as compared to Capsicum annuum. Conclusions: These indexes strongly reveal the life phenomenon of the intracellular water metabolism in plant leaves. This study highlights that the online electrophysiological parameters promise a new sight for intracellular water metabolism in plants.

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“…This is the first time that we have shown that plant excitability is related to age. Similarly, Favre et al (2011) have shown different excitability in different Arabidopsis thaliana accessions. Here, we found that persistent Li ?…”

Section: Distinguishing Between Growth and Circumnutating Behaviourmentioning

confidence: 95%

Lithium distinguishes between growth and circumnutation and augments glutamate-induced excitation of Helianthus annuus seedlings

2015

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An effect of lithium (Li ? ) on growth, circumnutation, and glutamate-induced excitation in sunflower (Helianthus annuus L.) seedlings was investigated using time-lapse photography and extracellular electrical potential measurements. The seedlings were treated with a micro and millimolar concentration of lithium chloride (LiCl) both persistently in a hydroponic medium and acutely through Li ? injection. The length of hypocotyls, fresh weight of seedlings, intensity and period of circumnutation, and the number of action potentials (APs) after glutamate (Glu) injection were determined. It was found that the circumnutation intensity and period did not depend on hypocotyl length and fresh weight of seedlings. Under persistent Li ? treatment, the circumnutation intensity was constant at a concentration between 0.2 and 20 mM although the hypocotyls were significantly shorter in relation to the control, whereas at a concentration of 40 mM circumnutation intensity decreased without any changes in the hypocotyl length. Under persistent treatment with 0.5 and 20 mM Li ? , the period of circumnutation was significantly prolonged. The number of APs in a Glu-induced series significantly increased in the seedlings exhibiting an Li ? -induced decrease in circumnutation intensity (in 40 and 60 mM Li ? ). Additionally Li ? injection before Glu injection also augmented the series of APs in seedlings growing without Li ? in hydroponic medium. These Li ? -sensitive responses demonstrated that circumnutation and growth are partly independent processes and reveal a relationship between circumnutation intensity and excitability in Helianthus annuus seedlings.

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Accession-dependent action potentials in Arabidopsis

Cited by 14 publications

References 56 publications

Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter

Imaging long distance propagating calcium signals in intact plant leaves with the BRET-based GFP-aequorin reporter

New sight: rapid monitor of intracellular water information in plant leaves based on electrophysiological parameters

Lithium distinguishes between growth and circumnutation and augments glutamate-induced excitation of Helianthus annuus seedlings

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