Acclimation of chloroplast transglutaminase to high NaClconcentration in a polyamine-deficient variant strain of Dunaliella salina and in its wild type

Dondini, Luca; Bonazzi, Stefania; Duca, Stefano Del; Bregoli, Anna Maria; Serafini-Fracassini, Donatella

doi:10.1078/0176-1617-00099

Cited by 34 publications

(31 citation statements)

References 40 publications

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“…The mechanistic details of PAs effects have not been clarified, although a proposed mechanism is based on PAs neutralizing harmful ROS in tissues or cells and inducing the reorganization of the photosynthetic apparatus. Several results have confirmed that the increase in intra-cellular polyamine content or polyamine metabolism related enzyme activity played an important role in photosynthesis of plants and in oxidative stress resistance (Dondini et al, 2001;Cochόn et al, 2007;Demetriou et al, 2007). Polyamines and especially the thylakoid-associated polyamines play a decisive role in protecting photosynthetic apparatus against oxidative stress.…”

Section: Oxidative Stressmentioning

confidence: 59%

“…Its substrates are Rubisco and some antenna complexes of thylakoids, such as LHCII, CP29, CP26 and CP24 (Del Duca et al, 1994). In Dunaliella salina whole cells, TGase seems to play a role in the acclimimation to high salt concentrations under light condition, and the content of chlorophylla and b of chloroplast were enhanced, the amount of 68kD and 55kDa polypeptides was particularly high in algae already acclimated cells (Dondini et al, 2001). Recently, Ortigosa et al (2009) showed that the overexpression of maize plastidial transglutaminas (chlTGZ) in the young leaves of tobacco www.intechopen.com chloroplasts seemed to induce an imbalance between capture and utilization of light in photosynthesis.…”

Section: Introductionmentioning

confidence: 97%

“…Thus, there was strong indication that polyamines possessed a pivotal role in photosynthesis. Chloroplasts also contain high activities of several polyamine biosynthetic enzymes (Borrell et al1995;Andreadakis and Kotzabasis 1996) and transglutaminase (TGase) catalyzing the covalent binding of polyamines to proteins (Del-Duca et al, 1994;Dondini et al, 2001;DellaMea et al, 2004). Polyamines can covalently bind with thylakoid membrane proteins under the TGase form of protein-glutamyl-polyamine or protein-glutamyl-polyamines-glutamylprotein (Dondini et al, 2003), such as D1, D2 protein and cytochrome b6/f, together with the involvement of polyamine, could be important for stabilisation of molecular complexes in the thylakoid membranes of osmotically stressed oat leaves (Besford et al, 1993).…”

Section: Thylakoid Membrane Protein Complexesmentioning

confidence: 99%

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A Review: Polyamines and Photosynthesis

Shu¹,

Guo²,

Yuan³

2012

Advances in Photosynthesis - Fundamental Aspects

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Section: Oxidative Stressmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 97%

Section: Thylakoid Membrane Protein Complexesmentioning

confidence: 99%

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A Review: Polyamines and Photosynthesis

Shu¹,

Guo²,

Yuan³

2012

Advances in Photosynthesis - Fundamental Aspects

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“…Several Coomassie-positive bands observed at stage 2 disappeared as ageing progressed. However, it cannot be excluded that these bands are aggregated, as previously observed under stress condition (Del Duca et al, 2000;Dondini et al, 2001). This is suggested by the labeled products having elevated molecular mass formed during the period of maximum enzyme activity and the simultaneous formation of high molecular mass bands immunodetected by the TGase antibody.…”

Section: Tgase Activity During the Corolla Life Spanmentioning

confidence: 79%

The Acropetal Wave of Developmental Cell Death of Tobacco Corolla Is Preceded by Activation of Transglutaminase in Different Cell Compartments

et al. 2007

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The activity of transglutaminase (TGase), an enzyme responsible for polyamine conjugation to proteins, was analyzed in relationship to developmental cell death (DCD) during the flower life span stages of the tobacco (Nicotiana tabacum) corolla. As the DCD exhibits an acropetal gradient, TGase was studied in corolla proximal, medial, and distal parts. TGase was immunorecognized by three TGase antibodies; the main 58-kD band decreased during corolla life, whereas a 38-kD band localized progressively from basal to distal parts. The former was present in the soluble, microsomal, plastidial (together with the 38-kD band), and cell wall fractions. The endogenous TGase activity increased during DCD reaching a maximum soon after the corolla opening. The activity maximum shifted from proximal to distal part, preceding the DCD acropetal pattern. A similar activity increase was observed by the exogenous TGase substrate (histidine 6 -Xpr-green fluorescent protein). Subcellular activities were detected in (1) the microsomes, where TGase activity is in general higher in the proximal part, peaking at the corolla opening; (2) the soluble fraction, where it is present only in the proximal part at senescence; (3) the plastids, where it shows an increasing trend; and (4) cell walls, prevailing in the distal part and progressively increasing. These data suggest a relationship between DCD and TGase; the latter, possibly released in the cell wall through the Golgi vesicles, could cooperate to cell wall strengthening, especially at the abscission zone and possibly during corolla shape change. The plastid TGase, stabilizing the photosystems, could sustain the energy requirements for the senescence progression.

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“…18 ± 21 Plant TGases may be involved in growth by cell division, 7,22,23 in apical growth, 24 as well as in stress responses. 25 Evidence for their structural role comes from the identification of some of their substrates, e.g. actin and tubulin 24 or cell wall proteins, 21 and through its involvement in the polymerisation of Rubisco.…”

Section: Introductionmentioning

confidence: 99%

Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco (Nicotiana tabacum) flowers

et al. 2002

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Corolla life span of undetached flowers of Nicotiana tabacum was divided into stages from the closed corolla (stage 1) through anthesis (stage 5) to death (stage 9). Senescence began around stage 6 in the proximal part, concomitantly with DNA laddering. Nuclear blebbing, DNA laddering, cell wall modification, decline in protein, water, pigment content and membrane integrity were observed during senescence and PCD. Transglutaminase activity was measured as mono-and bis-derivatives of putrescine (mono-PU; bis-PU) and bis-derivatives of spermidine (bis-SD). Bis-derivatives decreased with the progression of senescence, while mono-PU increased during early senescence; derivatives were present in different amounts in the proximal and distal parts of the corolla. In excised flowers, exogenous spermine delayed senescence and PCD, and caused an increase in free and acid-soluble conjugated PA levels. Bis-PU was the most abundant PA-derivative before DNA laddering stage; thereafter, bis-PU generally decreased and mono-PU became the most abundant derivative.

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Acclimation of chloroplast transglutaminase to high NaClconcentration in a polyamine-deficient variant strain of Dunaliella salina and in its wild type

Cited by 34 publications

References 40 publications

A Review: Polyamines and Photosynthesis

A Review: Polyamines and Photosynthesis

The Acropetal Wave of Developmental Cell Death of Tobacco Corolla Is Preceded by Activation of Transglutaminase in Different Cell Compartments

Transglutaminase activity during senescence and programmed cell death in the corolla of tobacco (Nicotiana tabacum) flowers

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