Acclimation of white lupin to phosphorus deficiency involves enhanced expression of genes related to organic acid metabolism

Uhde‐Stone, Claudia; Gilbert, Glena A.; Johnson, Jane M. F.; Litjens, Ralph; Zinn, Kelly E.; Temple, Stephen J.; Vance, Carroll P.; Allan, Deborah L.

doi:10.1023/a:1022335519879

Cited by 112 publications

(75 citation statements)

References 67 publications

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“…S7). This is not surprising, since levels of PEPC protein and phosphorylation status and/or activity are known to be markedly up-regulated in response to Pi deprivation in a wide range of plant species (Duff et al, 1989;Johnson et al, 1996;Uhde-Stone et al, 2003;Vance et al, 2003;Gregory et al, 2009;O'Leary et al, 2011a;Plaxton and Tran, 2011). Immunoblots of extracts prepared from meristematic root tips of +Pi and -Pi plants had equivalent ratios of immunoreactive p110: p107 subunits.…”

Section: Influence Of Pi Deprivation On Pepc Abundance Activity Andmentioning

confidence: 93%

“…This suggests that PEPC might have been activated by some type of PTM. In the wellstudied model crop white lupin (Lupinus albus), optimal PEPC activity also increases as its proteoid roots attain maturity (Johnson et al, 1996;Massonneau et al, 2001;Uhde-Stone et al, 2003).…”

Section: Influence Of Proteoid Root Development On Key Enzymes Of Orgmentioning

confidence: 99%

“…S1), a common abiotic stress that frequently limits plant growth in natural ecosystems. The marked induction of Class-1 PEPCs during Pi stress has been linked to the synthesis and excretion of large amounts of organic acid anions by roots of Pi-starved (-Pi) plants (O'Leary et al, 2011a;Uhde-Stone et al, 2003;Vance et al, 2003;Shane et al, 2004a). The excreted organic acids chelate metal cations such as Al 3+ and Ca 2+ that immobilize Pi in the soil, thus increasing soluble Pi concentrations by up to 1,000-fold .…”

mentioning

confidence: 99%

“…The possible contribution of PTMs such as phosphorylation to the in vivo activation of proteoid root PEPCs is currently unclear (e.g. see Uhde-Stone et al, 2003). However, this is feasible since the pronounced induction of PPCK genes coupled with the reversible phosphorylation-activation of a Class-1 PEPC isozyme (AtPPC1) has been conclusively demonstrated in -Pi Arabidopsis (Arabidopsis thaliana) suspension cells and seedlings (Gregory et al, 2009).…”

mentioning

confidence: 99%

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Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea

2013

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Accumulating evidence indicates important functions for phosphoenolpyruvate (PEP) carboxylase (PEPC) in inorganic phosphate (Pi)-starved plants. This includes controlling the production of organic acid anions (malate, citrate) that are excreted in copious amounts by proteoid roots of nonmycorrhizal species such as harsh hakea (Hakea prostrata). This, in turn, enhances the bioavailability of mineral-bound Pi by solubilizing Al 3+ , Fe 3+ , and Ca 2+ phosphates in the rhizosphere. Harsh hakea thrives in the nutrient-impoverished, ancient soils of southwestern Australia. Proteoid roots from Pi-starved harsh hakea were analyzed over 20 d of development to correlate changes in malate and citrate exudation with PEPC activity, posttranslational modifications (inhibitory monoubiquitination versus activatory phosphorylation), and kinetic/allosteric properties. Immature proteoid roots contained an equivalent ratio of monoubiquitinated 110-kD and phosphorylated 107-kD PEPC polypeptides (p110 and p107, respectively). PEPC purification, immunoblotting, and mass spectrometry indicated that p110 and p107 are subunits of a 430-kD heterotetramer and that they both originate from the same plant-type PEPC gene. Incubation with a deubiquitinating enzyme converted the p110:p107 PEPC heterotetramer of immature proteoid roots into a p107 homotetramer while significantly increasing the enzyme's activity under suboptimal but physiologically relevant assay conditions. Proteoid root maturation was paralleled by PEPC activation (e.g. reduced K m [PEP] coupled with elevated I 50 [malate and Asp] values) via in vivo deubiquitination of p110 to p107, and subsequent phosphorylation of the deubiquitinated subunits. This novel mechanism of posttranslational control is hypothesized to contribute to the massive synthesis and excretion of organic acid anions that dominates the carbon metabolism of the mature proteoid roots.

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Section: Influence Of Pi Deprivation On Pepc Abundance Activity Andmentioning

confidence: 93%

Section: Influence Of Proteoid Root Development On Key Enzymes Of Orgmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea

2013

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“…The evidence to support metabolic specialization comes predominately from studies with L. albus (Johnson et al, 1994(Johnson et al, , 1996a(Johnson et al, , 1996bKeerthisinghe et al, 1998;Neumann and Rö mheld, 1999;Neumann et al, 1999;Evans, 1999a, 1999b), and is based on three sets of observations: (1) genes involved in citrate synthesis are up-regulated when citrate exudation occurs from cluster roots (e.g. phosphoenolpyruvate carboxylase [PEPC]; Neumann et al, 1999;Kania et al, 2003;Uhde-Stone et al, 2003); (2) genes involved in citrate catabolism are down-regulated when citrate exudation occurs from cluster roots (e.g. aconitase; Neumann et al, 1999); and (3) transport, rather than net synthesis, is the rate-determining step for carboxylate exudation (Watt and Evans, 1999a).…”

mentioning

confidence: 99%

Developmental Physiology of Cluster-Root Carboxylate Synthesis and Exudation in Harsh Hakea. Expression of Phosphoenolpyruvate Carboxylase and the Alternative Oxidase

Shane¹,

Cramer²,

et al. 2004

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Harsh hakea (Hakea prostrata R.Br.) is a member of the Proteaceae family, which is highly represented on the extremely nutrientimpoverished soils in southwest Australia. When phosphorus is limiting, harsh hakea develops proteoid or cluster roots that release carboxylates that mobilize sparingly soluble phosphate in the rhizosphere. To investigate the physiology underlying the synthesis and exudation of carboxylates from cluster roots in Proteaceae, we measured O 2 consumption, CO 2 release, internal carboxylate concentrations and carboxylate exudation, and the abundance of the enzymes phosphoenolpyruvate carboxylase and alternative oxidase (AOX) over a 3-week time course of cluster-root development. Peak rates of citrate and malate exudation were observed from 12-to 13-d-old cluster roots, preceded by a reduction in cluster-root total protein levels and a reduced rate of O 2 consumption. In harsh hakea, phosphoenolpyruvate carboxylase expression was relatively constant in cluster roots, regardless of developmental stage. During cluster-root maturation, however, the expression of AOX protein increased prior to the time when citrate and malate exudation peaked. This increase in AOX protein levels is presumably needed to allow a greater flow of electrons through the mitochondrial electron transport chain in the absence of rapid ATP turnover. Citrate and isocitrate synthesis and accumulation contributed in a major way to the subsequent burst of citrate and malate exudation. Phosphorus accumulated by harsh hakea cluster roots was remobilized during senescence as part of their efficient P cycling strategy for growth on nutrient impoverished soils.In some plant species, a shortage of phosphorus induces the development of dense clusters of determinate branch roots (rootlets) that arise, en masse, from a localized region of the parent root axis. These short-lived structures have been termed proteoid roots because they were first described for Proteaceae (Purnell, 1960) but have since been found in a wide range of other species and families and are now often referred to as cluster roots . Most of our advances in cluster-root biology have been derived from studies of the crop species Lupinus albus (Fabaceae family) (Gardner et al

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Phosphorus as a Critical Macronutrient

Vance¹

2011

The Molecular and Physiological Basis of Nutrient Use Efficiency in Crops

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Acclimation of white lupin to phosphorus deficiency involves enhanced expression of genes related to organic acid metabolism

Cited by 112 publications

References 67 publications

Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea

Reciprocal Control of Anaplerotic Phosphoenolpyruvate Carboxylase by in Vivo Monoubiquitination and Phosphorylation in Developing Proteoid Roots of Phosphate-Deficient Harsh Hakea

Developmental Physiology of Cluster-Root Carboxylate Synthesis and Exudation in Harsh Hakea. Expression of Phosphoenolpyruvate Carboxylase and the Alternative Oxidase

Phosphorus as a Critical Macronutrient

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