Acclimation to and biodegradation of nitrilotriacetate (NTA) at trace concentrations in natural waters

Larson, Robert J.; Davidson, Daniel

doi:10.1016/0043-1354(82)90236-6

Cited by 35 publications

(27 citation statements)

References 15 publications

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“…Half-lives for NTA in Canadian (Williamsburg) groundwater agreed fairly well with previously reported values for Canadian surface waters [48], indicating the presence of a welladapted population of NTA degraders in the Canadian groundwater tested. Firstorder kinetics were observed, since initial concentrations were less than K, in all cases, i.e., in the linear range of the velocity curve where rates of degradation were directly proportional to concentration.…”

Section: Discussionsupporting

confidence: 88%

Metabolic Diversity and Activity of Heterotrophic Bacteria in Ground Water

Ventullo¹,

Larson²

1985

Environ Toxicol Chem

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The number and metabolic diversity of bacteria were studied in groundwaters collected in the United States and Canada. Bacterial numbers were determined by acridine orange direct counts, viable plate counts and 14C most‐probable‐number counts. Metabolic diversity was determined by heterotrophic activity and biodegradation potential assays using several classes of natural and xenobiotic substrates. Rates of metabolism in diversity studies (uptake and/or mineralization to carbon dioxide) were measured by radiotracer techniques and rate data were analyzed by nonlinear regression models to generate appropriate kinetic constants. Overall, the density and activity of groundwater bacteria were comparable to values reported for oligotrophic marine and freshwater systems. Bacterial numbers ranged from approximately 102 to 106 cells/ml, depending on the enumeration technique used and the particular sample tested. Turnover times for natural compounds in heterotrophic activity assays (i.e., glucose, amino acids) ranged from less than 100 to more than 2,000 h. Degradation half‐lives for xenobiotic and natural substrates in biodegradation potential studies exhibited a comparable range of values. In general, our results indicate that groundwater bacteria have the potential to metabolize several classes of natural and xenobiotic substrates, although at reduced rates compared with those of surface water systems.

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Section: Discussionsupporting

confidence: 88%

Metabolic Diversity and Activity of Heterotrophic Bacteria in Ground Water

Ventullo¹,

Larson²

1985

Environ Toxicol Chem

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“…Firstorder kinetics were observed, since initial concentrations were less than K, in all cases, i.e., in the linear range of the velocity curve where rates of degradation were directly proportional to concentration. Half-lives for NTA in Canadian (Williamsburg) groundwater agreed fairly well with previously reported values for Canadian surface waters [48], indicating the presence of a welladapted population of NTA degraders in the Canadian groundwater tested. Degradation of 2,4-D was not observed in either of the Canadian groundwater samples tested, although rapid degradation .…”

Section: Discussionsupporting

confidence: 88%

Metabolic diversity and activity of heterotrophic bacteria in ground water

Ventullo

Larson

1985

Enviro Toxic and Chemistry

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The number and metabolic diversity of bacteria were studied in groundwaters collected in the United States and Canada. Bacterial numbers were determined by acridine orange direct counts, viable plate counts and I4C most-probable-number counts. Metabolic diversity was determined by heterotrophic activity and biodegradation potential assays using several classes of natural and xenobiotic substrates. Rates of metabolism in diversity studies (uptake and/or mineralization to carbon dioxide) were measured by radiotracer techniques and rate data were analyzed by nonlinear regression models to generate appropriate kinetic constants. Overall, the density and activity of groundwater bacteria were comparable to values reported for oligotrophic marine and freshwater systems. Bacterial numbers ranged from approximately lo2 to lo6 cells/ml, depending on the enumeration technique used and the particular sample tested. Turnover times for natural compounds in heterotrophic activity assays (i.e., glucose, amino acids) ranged from less than 100 to more than 2,000 h. Degradation half-lives for xenobiotic and natural substrates in biodegradation potential studies exhibited a comparable range of values. In general, our results indicate that groundwater bacteria have the potential to metabolize several classes of natural and xenobiotic substrates, although at reduced rates compared with those of surface water systems.

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“…This is consistent with other research showing that the threshold chemical concentration that can produce adaptation may be quite low. For example, adaptation to nitrilotriacetic acid and C-12TMAC has been observed at initial exposure levels as low as 1 pg/L [5,9]. However, despite the adaptation in the presence of very low background surfactant concentrations, the response to C-12TMAC could not be maintained in the absence of the surfactant.…”

Section: Resultsmentioning

confidence: 99%