Acclimatization of Pisum sativum L., grown in soil contaminated with veterinary antibiotics, an attribute of dose hormetic response of root metabolites

Tasho, Reep Pandi; Shin, Won-Tae; Cho, J.Y.

doi:10.1016/j.scitotenv.2018.04.101

Cited by 11 publications

(3 citation statements)

References 19 publications

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“…Such mycorrhiza-mediated changes in the chemical composition of leaf tissues can impart phytoprotection against different abiotic stresses (Schweiger et al, 2014; Schweiger & Mueller, 2015). In P. sativum the studies were mainly focused on the seed metabolome, as was expected for a pulse crop, and a lot of attention was also paid to the effects of different stress conditions (Charlton et al, 2008; Vigeolas et al, 2008; Cechová et al, 2017; Hradilová et al, 2017; Sistani et al, 2017; Woźniak et al, 2017; Ellis et al, 2018; Tasho, Shin & Cho, 2018). However, Desalegn et al (2016) recently reported on leaf metabolome changes driven by inoculation with AMF and rhizobia of healthy pea plants and those infected with a pathogenic fungus Didymella pinodes .…”

Section: Introductionmentioning

confidence: 99%

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Shtark

Puzanskiy

Avdeeva

et al. 2019

PeerJ

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Arbuscular mycorrhiza (AM) is known to be a mutually beneficial plant-fungal symbiosis; however, the effect of mycorrhization is heavily dependent on multiple biotic and abiotic factors. Therefore, for the proper employment of such plant-fungal symbiotic systems in agriculture, a detailed understanding of the molecular basis of the plant developmental response to mycorrhization is needed. The aim of this work was to uncover the physiological and metabolic alterations in pea (Pisum sativum L.) leaves associated with mycorrhization at key plant developmental stages. Plants of pea cv. Finale were grown in constant environmental conditions under phosphate deficiency. The plants were analyzed at six distinct time points, which corresponded to certain developmental stages of the pea: I: 7 days post inoculation (DPI) when the second leaf is fully unfolded with one pair of leaflets and a simple tendril; II: 21 DPI at first leaf with two pairs of leaflets and a complex tendril; III: 32 DPI when the floral bud is enclosed; IV: 42 DPI at the first open flower; V: 56 DPI when the pod is filled with green seeds; and VI: 90–110 DPI at the dry harvest stage. Inoculation with Rhizophagus irregularis had no effect on the fresh or dry shoot weight, the leaf photochemical activity, accumulation of chlorophyll a, b or carotenoids. However, at stage III (corresponding to the most active phase of mycorrhiza development), the number of internodes between cotyledons and the youngest completely developed leaf was lower in the inoculated plants than in those without inoculation. Moreover, inoculation extended the vegetation period of the host plants, and resulted in increase of the average dry weight per seed at stage VI. The leaf metabolome, as analyzed with GC-MS, included about three hundred distinct metabolites and showed a strong correlation with plant age, and, to a lesser extent, was influenced by mycorrhization. Metabolic shifts influenced the levels of sugars, amino acids and other intermediates of nitrogen and phosphorus metabolism. The use of unsupervised dimension reduction methods showed that (i) at stage II, the metabolite spectra of inoculated plants were similar to those of the control, and (ii) at stages IV and V, the leaf metabolic profiles of inoculated plants shifted towards the profiles of the control plants at earlier developmental stages. At stage IV the inoculated plants exhibited a higher level of metabolism of nitrogen, organic acids, and lipophilic compounds in comparison to control plants. Thus, mycorrhization led to the retardation of plant development, which was also associated with higher seed biomass accumulation in plants with an extended vegetation period. The symbiotic crosstalk between host plant and AM fungi leads to alterations in several biochemical pathways the details of which need to be elucidated in further studies.

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Section: Introductionmentioning

confidence: 99%

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Shtark

Puzanskiy

Avdeeva

et al. 2019

PeerJ

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“…It was estimated that the average usage of VAs in China has reached about 6000 metric tons per year with continuous growth . The discharged antibiotics may enter the soil through land application of manures in the farmland and further release into the aquatic environment by runoff, driftage, or leaching. , In recent years, researchers have evidenced that the presence of antibiotics and antibiotic resistance genes in wastewater have a great possibility to disturb the natural ecological balance and result in an increase of resistant bacteria in the natural environment, posing a serious threat to ecosystems and human health. , Among these VAs, florfenicol (FF) is a synthetically produced broad-spectrum antibacterial agent and is especially developed for veterinary application . As one of the few allowed VAs in aquaculture, FF can exist for a long time in the environment and traditional water treatment technologies cannot effectively degrade or remove it .…”

Section: Introductionmentioning

confidence: 99%

Enhanced Removal of Veterinary Antibiotic Florfenicol by a Cu-Based Fenton-like Catalyst with Wide pH Adaptability and High Efficiency

et al. 2019

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The study on the removal of refractory veterinary antibiotic florfenicol (FF) in water is still very limited. In this study, an efficient Fenton-like catalyst was developed by synthesizing a series of Cu-based multi-metal layered double hydroxides (CuNiFeLa-LDHs) to degrade FF in aqueous solution. In the experiments, the screened CuNiFeLa-2-LDH with the molar ratio of La 3+ /(Fe 3+ + La 3+ ) = 0.1 exhibited high catalytic activity, achieving almost complete degradation of 5 mg L –1 FF under 5 mmol L –1 H 2 O 2 conditions. The mechanisms revealed that the enhanced catalytic performance was ascribed to the existence of Ni which accelerated the electron transfer rate and La which served as a Lewis acidic site to provide more reactive sites in this Cu-dominated Fenton-like reaction, further generating • OH, • O 2 – , and O 2 1 as active species to attack pollutants directly. Interestingly, the catalyst showed a wide pH adaptability and little release of copper ions to the solution. The regenerated CuNiFeLa-2-LDH is demonstrated to be a stable and reliable material for florfenicol degradation.

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“…For China, the MRL of kanamycin in milk is 200 μg kg -1 , while in Europe the limit is 150 μg kg -1 . [3] The current detection methods such as gas chromatography, high performance liquid chromatography (HPLC), [4][5][6] which have been listed as standard methods in most countries and regions, are limited by some shortcomings, such as the inability to quickly test in the field, complicated operation, and long test cycle, etc. Although new methods such as colorimetry [7] and electrochemistry [8] can achieve high-sensitivity detection of low-level kanamycin, they are susceptible to external interference and the electrode preparation is relatively complicated.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence assay for kanamycin based on target recycling strategy

Zhang

Jin

et al. 2022

Luminescence

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A chemiluminescence (CL) sensing strategy for kanamycin residue detection in fish samples was established based on luminol-functionalized gold nanoparticles as CL nanoprobe materials combined with DNA hairpin structure and carboxyl-modified magnetic beads. Relying on nucleic acid amplification technology, the system can successfully realize the recycling of kanamycin, so that the biosensor can release a large number of luminol-functionalized gold nanoparticles with excellent CL performance even at a low residual levels of kanamycin. The biosensor strategy showed a good linear relationship with kanamycin in the range 0.09-130 nM, the detection limit was as low as 0.04 nM. This method proves the excellent performance of the sensing strategy and provides a low-cost and high-sensitivity CL analysis strategy for the detection of kanamycin and even other antibiotics.

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Acclimatization of Pisum sativum L., grown in soil contaminated with veterinary antibiotics, an attribute of dose hormetic response of root metabolites

Cited by 11 publications

References 19 publications

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Metabolic alterations in pea leaves during arbuscular mycorrhiza development

Enhanced Removal of Veterinary Antibiotic Florfenicol by a Cu-Based Fenton-like Catalyst with Wide pH Adaptability and High Efficiency

Chemiluminescence assay for kanamycin based on target recycling strategy

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