Acclimatization of Pouteria gardeneriana Radlk micropropagated plantlets: Role of in vitro rooting and plant growth–promoting bacteria

Leite, Mariluza Silva; Pinto, Tainara Eler Furtado; Centofante, Agda Rabelo; Neto, Aurélio Rúbio; Silva, Fabiano Guimarães; Selari, Priscila Jane Romano Gonçalves; Martins, Paulo Sodero

doi:10.1016/j.cpb.2021.100209

Cited by 12 publications

(4 citation statements)

References 47 publications

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“…Biotization with endophytes is an attractive new strategy to protect plants that are micropropagated, avoiding mortality when transferred from in vitro to ex vitro conditions. Micropropagated plants inoculated with endophyte bacteria exhibit physiological and developmental changes and pathogen resistance [ 93 , 94 , 95 , 96 ]. It is evident that reintroducing these bacteria improves stress tolerance and promotes growth and establishment in the soil.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Martínez-Rodríguez

Beltran-Garcia

Salas

et al. 2022

Biology

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A. marmorata is the raw material used for tepextate mescal production but is classified as an endangered species. In the present study, we obtain and multiply clonal lines of Agave marmorata Roezl by selecting seedlings derived from seeds. Ten seedlings from two lots of 400 germinated seeds were selected for axillary bud proliferation induced by BAP 5 mg/L in vitamin-free Murashige and Skoog’s medium. Differences in shoot numbers, heights and senescent tissue formation were observed. Notably, the AM32 line formed 84 shoots and presented low senescent tissue after 60 d of culture. We also selected the AM31 and AM33 clonal lines. Four-month shoots were extracted with 80% methanol in water to determine the total content of saponins, flavonoids, and phenolic acids and compare the three clonal lines. Some bioactive molecules were identified using HPLC techniques and MALDI-TOF mass spectrometry none showed significant differences in content. Additionally, plants derived from the clonal lines were inoculated with four endophytic bacteria. Among these, Achromobacter xylosoxidans supported plant growth of AM32. A notable effect of plant death was observed after inoculation with Enterobacter cloacae, an endophyte of A. tequilana. Additionally, Pseudomonas aeruginosa, an endophyte from A. marmorata, reduced biomass. Our results demonstrate the incompatibility of A. marmorata to E. cloacae and specialization between the host plant and its endophytes. The compatibility of the plant-endophyte could be exploited to boost the establishment and stability of mutualisms to benefit plant development, stress tolerance and pathogen resistance. The differences in multiplication capacity, stable tissue formation, and endophyte biotization responses may indicate genetic variability. Clonal selection and micropropagation from seed-derived plants could contribute to conserving the endangered A. marmorata plant for reforestation in their natural habitats, thus, assuring mass propagation for sustainable industrial production of mescal, bioactive compounds, and prebiotics.

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Section: Discussionmentioning

confidence: 99%

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Martínez-Rodríguez

Beltran-Garcia

Salas

et al. 2022

Biology

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“…The diameter of the holes gradually increased over the 5 days, starting with 0.5 cm for the first 3 days and then enlarging to 1 cm for the last 2 days). This contributed to the establishment of transpiration processes in drier air compared with high humidity in vitro (Leite et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Tytarenko,

Tesliuk,

Merlich

et al. 2023

bta

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Clonal micropropagation is an effective method for plant reproduction, applicable in both scientific and industrial domains. However, a significant number of microclones are lost during the ex vitro acclimatization process. To address this, the introduction of beneficial microorganisms into the rhizosphere of micropropagated plants could have a positive effect on the survival rates and external characteristics of acclimatized plantlets. The aim of this study was to determine the protective and growth-promoting potential of Enterococcus italicus ONU547 and its effect on micropropagated plants during acclimatization. The antagonistic activity of the bacteria was determined using the agar block method. Lepidium sativum L. seeds were inoculated with bacterial suspensions at concentrations of 10 6 , 10 7 , and 10 8 CFU/ml. Subsequently, the roots of the microclones were treated with suspensions of 10 6 and 10 7 CFU/ml, and biometric characteristics were measured. The results demonstrated antagonistic properties against various phytopathogenic fungi, including Aspergillus niger , Cladosporium cladosporioides , Alternaria alternata , Alternaria tenuissima , Rhizoctonia cerealis , Penicillium expansum , and Paecilomyces variotii . Inoculation of L. sativum L. seeds resulted in improved germination rates, increased root numbers, and enhanced root and shoot lengths. Similarly, the effects of the studied bacteria on Rubus fruticosus L. and Paulownia tomentosa Steud. during the acclimatization stage led to higher survival rates, increased shoot lengths, greater node numbers, and larger leaf areas. A concentration of 10 7 CFU/ml was identified as optimal for inoculating the microclones. The findings indicate that E. italicus ONU547 holds promise for the inoculation of micropropagated plants during the acclimatization process. Further research is recommended to establish the specific interaction mechanisms between these bacteria and plants.

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“…For in vitro plants to successfully take root in non-sterile conditions, they must be ready to overcome the stresses to which they are exposed during the adaptation process. According to the data of the authors (Bag, 2019;Leite, 2021), to increase the percentage of survival of plants in vivo, it is necessary to start preparing them for new conditions in vitro, including a decrease in air humidity. In this regard, the quality and physical properties of the nutrient medium are of paramount importance.…”

Section: Figure 3 the Content Of Dry Matter And Water In The Tissues ...mentioning

confidence: 99%

Influence of nutrient media on the physiological parameters of grape microclones

Zelenianska¹,

Ishchenko²,

Samofalov³

2023

Scientific Horizons

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Successful rooting of grape microclones in uncontrolled environmental conditions (in vivo) depends on the level of resistance, which is formed at the stage of their passage and growth in vitro. An important role in this case is played by indicators of the water regime of the vegetative mass. The purpose of this study was to get acquainted with the results of determining the water-holding capacity, the intensity of transpiration of grape microclones in vitro and to establish the share of their influence on the adaptation potential in vivo. Biotechnological, laboratory, vegetation, and mathematical and statistical research methods were used in this study. The obtained results showed that to optimize the physiological processes in the tissues of the leaves and shoots of grape microclones, to increase their viability under in vivo conditions, it is advisable to cultivate them in vitro on structured nutrient media (MS + agroperlite, MS + vermiculite, MS + agroperlite + vermiculite) with the content of phytohormones IAA – 0.2 mg/l, and 6-BAP – 0.3 mg/l. Structured nutrient media contributed to an increase in water retention capacity and a decrease in the intensity of transpiration of tissues of leaves and shoots of microclones of both technical and rootstock varieties. During 60 minutes of research, from 0.006 g to 0.034 g of water evaporated from microclones of technical varieties, from 0.003 g to 0.053 g from microclones of rootstock varieties, respectively. Transpiration intensity (after 10 min) decreased by 1.7-1.8 times. On the control Murashige and Skoog nutrient medium, plants evaporated a larger amount of water during the corresponding period of time: from 0.006 g to 0.079 g (technical varieties) and from 0.008 g to 0.086 g (rootstock varieties); the intensity of transpiration was higher. After cultivation of grape microclones on structured nutrient media, they were characterized by a higher content of dry matter in the tissues of leaves and shoots (14.6-15.0%) and better survival rates in vivo (76.3-98.5%, at 58.5-65.2% in control). The reliability of the results obtained is confirmed by the results of multivariate analysis of variance. The obtained results expand the understanding of dynamic changes in the indicators of the water regime of the vegetative mass of grape microclones in vitro, their influence on plant survival under in vivo conditions

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Acclimatization of Pouteria gardeneriana Radlk micropropagated plantlets: Role of in vitro rooting and plant growth–promoting bacteria

Cited by 12 publications

References 47 publications

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Micropropagation of Seed-Derived Clonal Lines of the Endangered Agave marmorata Roezl and Their Compatibility with Endophytes

Impact of Enterococcus italicus ONU547 on the growth and acclimatization of micropropagated Rubus fruticosus L. and Paulownia tomentosa Steud. plants to ex vitro conditions

Influence of nutrient media on the physiological parameters of grape microclones

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