Accommodation of powdery mildew fungi in intact plant cells

Eichmann, Ruth; Hückelhoven, Ralph

doi:10.1016/j.jplph.2007.05.004

Cited by 71 publications

(49 citation statements)

References 126 publications

(163 reference statements)

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“…This is accompanied by rearrangements of F-actin, MTs, endomembranes, and the plasma membrane (Genre and Bonfante, 2002;Genre et al, 2005;Koh et al, 2005;Opalski et al, 2005;Eichmann and Hü ckelhoven, 2008). Our data support that ROPs and ROP regulatory proteins are involved in organization of the cytoskeleton for accommodation of fungal structures within intact plant cells.…”

Section: The C Terminus Associates Magap1 To Mtssupporting

confidence: 72%

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

et al. 2011

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Little is known about the function of host factors involved in disease susceptibility. The barley (Hordeum vulgare) ROP (RHO of plants) G-protein RACB is required for full susceptibility of the leaf epidermis to invasion by the biotrophic fungus Blumeria graminis f. sp hordei. Stable transgenic knockdown of RACB reduced the ability of barley to accommodate haustoria of B. graminis in intact epidermal leaf cells and to form hairs on the root epidermis, suggesting that RACB is a common element of root hair outgrowth and ingrowth of haustoria in leaf epidermal cells. We further identified a barley MICROTUBULE-ASSOCIATED ROP-GTPASE ACTIVATING PROTEIN (MAGAP1) interacting with RACB in yeast and in planta. Fluorescent MAGAP1 decorated cortical microtubules and was recruited by activated RACB to the cell periphery. Under fungal attack, MAGAP1-labeled microtubules built a polarized network at sites of successful defense. By contrast, microtubules loosened where the fungus succeeded in penetration. Genetic evidence suggests a function of MAGAP1 in limiting susceptibility to penetration by B. graminis. Additionally, MAGAP1 influenced the polar organization of cortical microtubules. These results add to our understanding of how intact plant cells accommodate fungal infection structures and suggest that RACB and MAGAP1 might be antagonistic players in cytoskeleton organization for fungal entry.

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Section: The C Terminus Associates Magap1 To Mtssupporting

confidence: 72%

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

et al. 2011

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“…However, a relative transcript decrease to 0.4 occurred between 8 and 16 HAI, lasting throughout the experiment. This ARFA1b transcript decrease coincided with the time point of penetration at 10 to 12 HAI (Zeyen et al, 2002;Eichmann and Hü ckelhoven, 2008). This transcript decrease might therefore be counteracted by constitutive expression of ARFA1b/1c, which reestablishes the GTPase activity level ( Figure 2B).…”

Section: Arfa1b/1c Is Required For Full Penetration Resistancementioning

confidence: 60%

“…Callose deposition in papillae is an early response to penetration attempts (Eichmann and Hü ckelhoven, 2008), and in Ingrid (wt), callose accumulation was detected at ;70% of the attack sites at 10 HAI ( Figure 5A). To study the timing of callose deposition relative to ARFA1b/1c assembly at Bgh appressoria penetration sites, the same Golden Promise leaf material that was assayed for ARFA1b/1c localization ( Figures 6B to 6F) was used in a time-course experiment for callose detection ( Figure 6G).…”

Section: Arfa1b/1c Localization Near Penetration Sites Prior To the Amentioning

confidence: 99%

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The Multivesicular Body-Localized GTPase ARFA1b/1c Is Important for Callose Deposition and ROR2 Syntaxin-Dependent Preinvasive Basal Defense in Barley

et al. 2010

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Host cell vesicle traffic is essential for the interplay between plants and microbes. ADP-ribosylation factor (ARF) GTPases are required for vesicle budding, and we studied the role of these enzymes to identify important vesicle transport pathways in the plant-powdery mildew interaction. A combination of transient-induced gene silencing and transient expression of inactive forms of ARF GTPases provided evidence that barley (Hordeum vulgare) ARFA1b/1c function is important for preinvasive penetration resistance against powdery mildew, manifested by formation of a cell wall apposition, named a papilla. Mutant studies indicated that the plasma membrane-localized REQUIRED FOR MLO-SPECIFIED RESISTANCE2 (ROR2) syntaxin, also important for penetration resistance, and ARFA1b/1c function in the same vesicle transport pathway. This was substantiated by a requirement of ARFA1b/1c for ROR2 accumulation in the papilla. ARFA1b/1c is localized to multivesicular bodies, providing a functional link between ROR2 and these organelles in penetration resistance. During Blumeria graminis f sp hordei penetration attempts, ARFA1b/1c-positive multivesicular bodies assemble near the penetration site hours prior to the earliest detection of callose in papillae. Moreover, we showed that ARFA1b/1c is required for callose deposition in papillae and that the papilla structure is established independently of ARFA1b/1c. This raises the possibility that callose is loaded into papillae via multivesicular bodies, rather than being synthesized directly into this cell wall apposition.

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“…Powdery mildew fungi infect a wide variety of monocots and dicots, forming feeding structures called haustoria in epidermal cells of their hosts (reviewed in Eichmann and Hü ckelhoven, 2008). In response to these fungi, plants reinforce their cell walls with callose-rich appositions, called papillae, at the site of attack.…”

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confidence: 99%

Linking Multivesicular Bodies to Resistance against Fungal Invasion

Hofmann

2010

Plant Cell

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Accommodation of powdery mildew fungi in intact plant cells

Cited by 71 publications

References 126 publications

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

A Barley ROP GTPase ACTIVATING PROTEIN Associates with Microtubules and Regulates Entry of the Barley Powdery Mildew Fungus into Leaf Epidermal Cells

The Multivesicular Body-Localized GTPase ARFA1b/1c Is Important for Callose Deposition and ROR2 Syntaxin-Dependent Preinvasive Basal Defense in Barley

Linking Multivesicular Bodies to Resistance against Fungal Invasion

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