Accomplishments and challenges in stem cell imaging in vivo

Bose, Rajendran J.C.; Mattrey, Robert F.

doi:10.1016/j.drudis.2018.10.007

Cited by 25 publications

(15 citation statements)

References 144 publications

(241 reference statements)

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“…While cell-based therapies provide promise for regulating regeneration, there are also significant drawbacks (e.g., variability in therapeutic efficacy, regulatory issues, low cell survivability). [65][66][67] Acellular biomaterials platforms that could replicate aspects of the immunomodulatory behavior of hMSCs may prove advantageous for the future of regenerative medicine. [68,69] We demonstrate that IL-10 is able to reduce macrophage pro-inflammatory activity, both when introduced solubly in the medium and when tethered directly to microgel scaffolds using a SPAAC reaction.…”

Section: Discussionmentioning

confidence: 99%

“…While biochemical stimuli, 59 porosity, 34,60,61 and polymer chemistry 62,63 have all been tailored to modulate immune cell‐biomaterials interactions, we specifically explored the ability of IL‐10‐functionalized scaffolds to modulate macrophage polarization. While cell‐based therapies provide promise for regulating regeneration, there are also significant drawbacks (e.g., variability in therapeutic efficacy, regulatory issues, low cell survivability) 64‐66 . Acellular biomaterials platforms that could replicate aspects of the immunomodulatory behavior of hMSCs may prove advantageous for the future of regenerative medicine 67,68 .…”

Section: Discussionmentioning

confidence: 99%

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Mesenchymal stem cell‐inspired microgel scaffolds to control macrophage polarization

Caldwell

Rao

Golden

et al. 2021

Bioengineering & Transla Med

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Mesenchymal stem cell‐inspired microgel scaffolds to control macrophage polarization

Caldwell

Rao

Golden

et al. 2021

Bioengineering & Transla Med

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“…MRI has a high spatial and temporal resolution, no tissue penetrating limit, and no radiation, but the disadvantages include relatively low sensitivity and low contrast, requiring a high load of cells with a magnetic label and comparatively long imaging times, with the possibility of affecting cell viability or biological interaction with the contrast agent [43,[62][63][64]. In this review, three studies used HSC tracking analysis by MRI with different magnetic fields, in which the highest magnetic field (17.6 Teslas) provided longer follow-ups (14 days after transplantation) when using low nanoparticle concentrations and incubation times in the cell labeling process.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Oliveira

Nucci

Filgueiras

et al. 2020

Cells

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The hematopoietic stem cell engraftment depends on adequate cell numbers, their homing, and the subsequent short and long-term engraftment of these cells in the niche. We performed a systematic review of the methods employed to track hematopoietic reconstitution using molecular imaging. We searched articles indexed, published prior to January 2020, in PubMed, Cochrane, and Scopus with the following keyword sequences: (Hematopoietic Stem Cell OR Hematopoietic Progenitor Cell) AND (Tracking OR Homing) AND (Transplantation). Of 2191 articles identified, only 21 articles were included in this review, after screening and eligibility assessment. The cell source was in the majority of bone marrow from mice (43%), followed by the umbilical cord from humans (33%). The labeling agent had the follow distribution between the selected studies: 14% nanoparticle, 29% radioisotope, 19% fluorophore, 19% luciferase, and 19% animal transgenic. The type of graft used in the studies was 57% allogeneic, 38% xenogeneic, and 5% autologous, being the HSC receptor: 57% mice, 9% rat, 19% fish, 5% for dog, porcine and salamander. The imaging technique used in the HSC tracking had the following distribution between studies: Positron emission tomography/single-photon emission computed tomography 29%, bioluminescence 33%, fluorescence 19%, magnetic resonance imaging 14%, and near-infrared fluorescence imaging 5%. The efficiency of the graft was evaluated in 61% of the selected studies, and before one month of implantation, the cell renewal was very low (less than 20%), but after three months, the efficiency was more than 50%, mainly in the allogeneic graft. In conclusion, our review showed an increase in using noninvasive imaging techniques in HSC tracking using the bone marrow transplant model. However, successful transplantation depends on the formation of engraftment, and the functionality of cells after the graft, aspects that are poorly explored and that have high relevance for clinical analysis.

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“…Unlike traditional indirect methods of cell labelling, such as superparamagnetic iron oxide (SPIO) and radionuclide labelling, reporter genes are inherited genetically (direct labelling) and can be used to monitor cell proliferation and survival for the lifetime of transplanted and their progeny cells. Current challenges and comparison of methods for stem cell tracking are reviewed . In case of fluorescent reporters, changes in fluorescent signal indicate cell death or proliferation.…”

Section: Generation Of Transplantable Porcine Pancreatic Islets and Hmentioning

confidence: 99%

Integration of nano‐ and biotechnology for beta‐cell and islet transplantation in type‐1 diabetes treatment

et al. 2020

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Regenerative medicine using human or porcine β-cells or islets has an excellent potential to become a clinically relevant method for the treatment of type-1 diabetes.High-resolution imaging of the function and faith of transplanted porcine pancreatic islets and human stem cell-derived beta cells in large animals and patients for testing advanced therapy medicinal products (ATMPs) is a currently unmet need for pre-clinical/clinical trials. The iNanoBIT EU H2020 project is developing novel highly sensitive nanotechnology-based imaging approaches allowing for monitoring of survival, engraftment, proliferation, function and whole-body distribution of the cellular transplants in a porcine diabetes model with excellent translational potential to humans. We develop and validate the application of single-photon emission computed tomography (SPECT) and optoacoustic imaging technologies in a transgenic insulindeficient pig model to observe transplanted porcine xeno-islets and in vitro differentiated human beta cells. We are progressing in generating new transgenic reporter pigs and human-induced pluripotent cell (iPSC) lines for optoacoustic imaging and

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Accomplishments and challenges in stem cell imaging in vivo

Cited by 25 publications

References 144 publications

Mesenchymal stem cell‐inspired microgel scaffolds to control macrophage polarization

Mesenchymal stem cell‐inspired microgel scaffolds to control macrophage polarization

Noninvasive Tracking of Hematopoietic Stem Cells in a Bone Marrow Transplant Model

Integration of nano‐ and biotechnology for beta‐cell and islet transplantation in type‐1 diabetes treatment

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