2016
DOI: 10.1002/btpr.2342
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Accounting for host cell protein behavior in anion‐exchange chromatography

Abstract: Host cell proteins (HCP) are a problematic set of impurities in downstream processing (DSP) as they behave most similarly to the target protein during separation. Approaching DSP with the knowledge of HCP separation behavior would be beneficial for the production of high purity recombinant biologics. Therefore, this work was aimed at characterizing the separation behavior of complex mixtures of HCP during a commonly used method: anion-exchange chromatography (AEX). An additional goal was to evaluate the perfor… Show more

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Cited by 3 publications
(1 citation statement)
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References 66 publications
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“…The mAb producer was used as an example of another cell line overexpressing a recombinant protein to provide an alternate control to that of a null cell line. As seen in Swanson et al IEX is an incredibly useful tool in clarifying complex mixtures of HCP, although to achieve an acceptable level of purification usually additional orthogonal techniques and depth filtration are used. In the case of GAA, the cysteine proteases Cathepsin B and Z are believed to be responsible for target molecule (GAA) degradation, and it is therefore important to track their expression, release and removal throughout the bioprocess.…”
Section: Introductionmentioning
confidence: 99%
“…The mAb producer was used as an example of another cell line overexpressing a recombinant protein to provide an alternate control to that of a null cell line. As seen in Swanson et al IEX is an incredibly useful tool in clarifying complex mixtures of HCP, although to achieve an acceptable level of purification usually additional orthogonal techniques and depth filtration are used. In the case of GAA, the cysteine proteases Cathepsin B and Z are believed to be responsible for target molecule (GAA) degradation, and it is therefore important to track their expression, release and removal throughout the bioprocess.…”
Section: Introductionmentioning
confidence: 99%