Accucary of epifluorescence microscopy counts for direct estimates of bacterial numbers

Lebaron, P.; Troussellier, M.; Got, Patrice

doi:10.1016/0167-7012(94)90039-6

Cited by 33 publications

(24 citation statements)

References 8 publications

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“…Similar significant correlations (R 2 > 0.8) were also found in the north-western Mediterranean Sea (Lebaron et al, 1993(Lebaron et al, , 1998. Bacterial counts obtained by flow cytometry and microscopy were more similar in the Adriatic Sea than in the English Channel and replicate experiments in both investigated areas showed that coefficients of variation were lower for bacterial counts estimated by FCM than by microscopy ( Figure 4).…”

Section: Resultssupporting

confidence: 61%

“…; std. dev) plot of bacterial abundance obtained by epifluorescence microscopy and flow cytometry from (A) the Adriatic Sea and (B) the English Channel Noted significant variations in bacterial abundance obtained by microscopy can be explained by the fact that presence of organic and mineral particles and the small sizes of most marine bacteria may result in lower bacterial discrimination (Lebaron et al, 1993;Gasol & Morán, 1999). Use of flow cytometry deals better with that problem because flow cytometer is separating bacteria from other particles on the basis of light scatter (size) and pigment content and has greater precision than microscopy counting (Sieracki et al, 1995;Monger & Landry, 1993;Joachimsthal et al, 2003;Chisholm et al, 1988).…”

Section: Resultsmentioning

confidence: 99%

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Identification and Characterisation of Microbial Populations Using Flow Cytometry in the Adriatic Sea

Šantić¹,

Krstulović²

2012

Flow Cytometry - Recent Perspectives

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Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Identification and Characterisation of Microbial Populations Using Flow Cytometry in the Adriatic Sea

Šantić¹,

Krstulović²

2012

Flow Cytometry - Recent Perspectives

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“…For detection, a FACSCalibur flow cytometer with standard laser and optics was used. For calibration of side scatter and green fluorescence signals, and as an internal standard for cytometric counts and measures, fluorescent latex beads (1.58 µm diameter) were systematically added at a known abundance to each sample (28). The apparent nucleic acid structure was also evaluated, and the results included total bacteria, the bacteria with high apparent nucleic acid content (HNA) and the bacteria with low apparent nucleic acid content (LNA) (see 17, 14 for details).…”

Section: Methodsmentioning

confidence: 99%

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean

Andrade¹,

Gonzalez²,

Rezende³

et al. 2007

Braz. J. Microbiol.

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Bacterioplankton was studied in a large area of Southwest Atlantic Ocean between 13 and 25ºS and 28 and 42ºW. Samples were collected in 108 stations at 20 m depth. Bacteria were enumerated by flow cytometry after nucleic acid staining with syto13 and two subgroups were differentiated: low nucleic acid content (LNA) and high nucleic acid content (HNA) bacteria. Total bacterial numbers varied from 0.37 to 5.53 10 5 cells mL -1. HNA cells represented 15 to 70% of the total number while LNA cells represented 30 to 85%. Heterotrophic bacterial production was determined by incorporation of tritiated leucine and ranged from 2.7 to 171.07 ng C L -1 h -1. No significant correlation was found between abundance and production. Nevertheless with support of multivariate analysis between bacterial abundance, bacterial production, chlorophyll a and other oceanographic data the distribution of the groups in two different oceanic provinces could be explained by nutrient availability. HNA bacteria accounted for the high percentage of cells found in the area north of 19ºS, linked to higher temperature waters and riverine nutrients inputs. LNA bacteria were the dominant cells south of this latitude and were correlated to the higher values of nitrate found for the same area.

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“…Counting was carried out after excitation at 490 nm and examination of 30 microscopic fields per slide. results are given for each experiment as the number of bacteria per mL of the original sample (LEBAroN et al, 1994;troUSSELLiEr et al, 1985).…”

Section: Cells Staining Analysismentioning

confidence: 99%

Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms

Mejri

Bour

Boukef

et al. 2008

rseau

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AbstractThe occurrence of the mesophilic motile Gram negative non enterobacterial speciesA. hydrophilain the wild is a major problem that deserves to be resolved since it is a potentially pathogen able to enter into a non-culturable state on routine bacteriological plating media. These non-culturable forms can be detected by several direct or indirect visualization methods. This species has frequently been isolated from pathological forms in fish farming marine areas, especially near wastewater discharges. Consequently, we studiedA. hydrophilain marine water microcosms placed during a 24 hour period in treated waste waters and compared with the homologous strain not placed in the same conditions. Thus, two kinds of microcosms were prepared using filtered and autoclaved marine water or waste water, inoculated byA. hydrophilaand maintained at 25°C in darkness. The results obtained indicated thatA. hydrophilapopulation incubated at 25°C in marine water declined rapidly (3.21 log units in plate count number) during the first three days. Additionally, we noted thatA. hydrophilaincubated in marine water after a previous treatment in waste water declined progressively to 2.74 log units (in plate count number). Nevertheless, we showed no significant variations of the number of total bacterial cells forA. hydrophiladeveloped in marine water after prior treatment in waste water, despite the appearance of the VBNC form. During this state, rods of normal size, elongated cells and cocci were obtained. Concomitantly, we determined several changes in biochemical and antimicrobial patterns of stressedA. hydrophila, notably the acquisition of adipate metabolization and an increase of resistance to antimicrobial compounds, especially forA. hydrophilaincubated in marine water after treatment in waste water.

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Accucary of epifluorescence microscopy counts for direct estimates of bacterial numbers

Cited by 33 publications

References 8 publications

Identification and Characterisation of Microbial Populations Using Flow Cytometry in the Adriatic Sea

Identification and Characterisation of Microbial Populations Using Flow Cytometry in the Adriatic Sea

Distribution of HNA and LNA bacterial groups in the Southwest Atlantic Ocean

Effect of Previous Incubation of Aeromonas hydrophila in Wastewater Prior to Its Transfer Into Marine Water Microcosms

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