Accumulation of Abscisic Acid in Cell Suspension Cultures of Oilseed Rape Treated with the Growth Retardant BAS 111 .. W: Effects on Osmotic Potential and Potassium, Water and Sugar Contents

Häuser, Christiane; Kwiatkowski, J.; Jung, Johannes; Großmann, Klaus

doi:10.1016/s0176-1617(11)81033-3

Cited by 12 publications

(3 citation statements)

References 18 publications

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“…Moreover, these growth retardants appear to exhibit different effects in influencing the endogenous ABA content (Grossmann 1990). Inereased ABA levels ui cell suspension cultures of oilseed rape (Hauser et al 1992) and in normal and water-stressed leaves of different species have been reported (Asare-Boamah et al 1986, Pan 1990, Zeevaart et al 1990. Tafazoli and Beyl 1993.…”

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confidence: 98%

Abscisic acid increases in non-growing and paclobutrazol-treated fruits of seedless mandarins

Zacarias¹,

Talón²,

Ben-Cheikh³

et al. 1995

Physiol Plant

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Zacarias, L., Talon, M., Ben-Cheikh, W., Lafuente, M. T. and Primo-Millo, E. 1995. Abscisic acid increases in non-growing and paclobutrazol-lreated fruits of seedless mandarins. -Physiol. Plant. 95: 613-619.Clementine [Citrus reticuiala fHoit.] Ex. Tanaka cv. Oroval) is a self-incompatible mandarin with a slow rale of fruit growth and high percentage of fruit abscission. Seedless Satsuma (Citrus unshiu [Mak] Marc. cv. Clausellina) shows natural parthenocarpy and higher fruit set. Application of 25 ng fruit ' of paclobutiazol (PP333), an inhibitor of gibberelhn biosynthesis, reduced the rate of growth and accelerated fruit abscission in both varieties. In contrast, gibberellin A, (GAj) stimulated fmit growth only in the selfincompatible mandarin. Clementine fruits, in the absence of pollination, showed an approximately 2-fold transient increase in the free abscisic acid (ABA) content shortly af-teT petal fall. In Satsuma, a very small accumulation of ABA was detected. Paclobutrazol treatment induced a 3-fold increase in ABA in Satsuma fruits but did not substantially affect the pattem of ABA accumulation in Clementine. In this variet>', GA, suppressed the ABA increase observed in untreated fruits. These effects were observed 24 h after treatment. However, in Satsuma fruits, the effect of GA, on the ABA content was negligible. In addition, a comparative analysis of growing and non-growing fruits of Clementine showed that ABA, on a per unit weight basis, was always higher in the nongrowing fruits. Treatment with 85 pM fluridoae. an inhibitor of carotenoid biosynthesis and thus indirectly of ABA, delayed fruit abscission in Clementine, but also decreased fmit growth. Collectively, these observations indicate a relationship between high ABA content and a reduced rate of fruit growth and an acceleration of fruit abscission. Kev words -Abscisic acid. Citrus reticulata. Citrus unshiu. fruit growth and abscission, gibberellins. mandarin, paclobutrazol. L. Zaearias (eorresponding author) and M. T. Lafuente. Ptiysiol. Ptant. 95. 1995 s\ -J-\

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confidence: 98%

Abscisic acid increases in non-growing and paclobutrazol-treated fruits of seedless mandarins

Zacarias¹,

Talón²,

Ben-Cheikh³

et al. 1995

Physiol Plant

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“…14,[24][25][26][27][28] In addition, triazoletype growth retardants are effective in protecting plants from various environmental stresses. 11) These results are fully consistent with our observations, suggesting that the CYP707A family ubiquitously regulates endogenous ABA levels in higher plants.…”

Section: Resultsmentioning

confidence: 99%

A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism inArabidopsis

Saito

Okamoto

Shinoda

et al. 2006

Bioscience, Biotechnology, and Biochemistry

114

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“…On the other hand, exogenous ABA application enhanced the levels of TAG’s (nine fold increase) in somatic embryos of Picea glauca and rapeseed cultures [ 7 , 10 ]. In addition, application of exogenous ABA increased the levels of total sugar content as well as reducing sugars in cultured seedling of Phaseolus vulgaris and in cell cultures of oilseed rape [ 11 , 12 ]. A 19 kDa oil body protein encoding oleosin and a desaturase lipid gene were found to be up regulated, correlating to the physiological changes after exogenous ABA treatment in B. napus embryo cultures [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling identifies ABA mediated regulatory changes towards storage filling in developing seeds of castor bean (Ricinus communis L.)

Chandrasekaran

Liu

2014

Cell Biosci

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BackgroundThe potential biodiesel plant castor bean (Ricinus communis) has been in the limelight for bioenergy research due to the availability of its genome which raises the bar for genome-wide studies claiming advances that impact the “genome-phenome challenge”. Here we report the application of phytohormone ABA as an exogenous factor for the improvement of storage reserve accumulation with a focus on the complex interaction of pathways associated with seed filling.ResultsAfter the application of exogenous ABA treatments, we measured an increased ABA levels in the developing seeds cultured in vitro using the ELISA technique and quantified the content of major biomolecules (including total lipids, sugars and protein) in treated seeds. Exogenous ABA (10 μM) enhanced the accumulation of soluble sugar content (6.3%) followed by deposition of total lipid content (4.9 %). To elucidate the possible ABA signal transduction pathways towards overall seed filling, we studied the differential gene expression analysis using Illumina RNA-Sequencing technology, resulting in 2568 (1507-up/1061-down regulated) differentially expressed genes were identified. These genes were involved in sugar metabolism (such as glucose-6-phosphate, fructose 1,6 bis-phosphate, glycerol-3-phosphate, pyruvate kinase), lipid biosynthesis (such as ACS, ACBP, GPAT2, GPAT3, FAD2, FAD3, SAD1 and DGAT1), storage proteins synthesis (such as SGP1, zinc finger protein, RING H2 protein, nodulin 55 and cytochrome P450), and ABA biosynthesis (such as NCED1, NCED3 and beta carotene). Further, we confirmed the validation of RNA-Sequencing data by Semi-quantitative RT-PCR analysis.ConclusionsTaken together, metabolite measurements supported by genes and pathway expression results indicated in this study provide new insights to understand the ABA signaling mechanism towards seed storage filling and also contribute useful information for facilitating oilseed crop functional genomics on an aim for utilizing castor bean agricultural and bioenergy use.

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Accumulation of Abscisic Acid in Cell Suspension Cultures of Oilseed Rape Treated with the Growth Retardant BAS 111 .. W: Effects on Osmotic Potential and Potassium, Water and Sugar Contents

Cited by 12 publications

References 18 publications

Abscisic acid increases in non-growing and paclobutrazol-treated fruits of seedless mandarins

Abscisic acid increases in non-growing and paclobutrazol-treated fruits of seedless mandarins

A Plant Growth Retardant, Uniconazole, Is a Potent Inhibitor of ABA Catabolism inArabidopsis

Transcriptome profiling identifies ABA mediated regulatory changes towards storage filling in developing seeds of castor bean (Ricinus communis L.)

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