1996
DOI: 10.1128/jvi.70.8.5100-5105.1996
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Accumulation of alfalfa mosaic virus RNAs 1 and 2 requires the encoded proteins in cis

Abstract: RNAs 1 and 2 of the tripartite genome of alfalfa mosaic virus (AlMV) encode the replicase proteins P1 and P2, respectively. P1 expressed in transgenic plants (P1 plants) can be used in trans to support replication of AlMV RNAs 2 and 3, and P2 expressed in transgenic plants (P2 plants) can be used in trans to support replication of AlMV RNAs 1 and 3. Wild-type RNA 1 was able to coreplicate with RNAs 2 and 3 in P1 plants, but this ability was abolished by frameshifts or deletions in the P1 gene of RNA 1. Similar… Show more

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Cited by 28 publications
(6 citation statements)
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“…5, the data indicate that sequences recognized by the RdRp in vitro in plus-strand AMV RNAs 1, 2, and 3 are confined to the 3Ј UTRs. Recently, evidence was presented indicating that the P1, P2, and CP genes play a cis-acting role in the replication of RNAs 1, 2, and 3, respectively (32,35). Our results indicate that minus-strand RNA synthesis in vitro does not require cis-acting sequences at internal positions of the AMV plus-strand RNAs, as has been reported for bacteriophage Q␤ RNA (14).…”
Section: Discussionsupporting
confidence: 82%
See 1 more Smart Citation
“…5, the data indicate that sequences recognized by the RdRp in vitro in plus-strand AMV RNAs 1, 2, and 3 are confined to the 3Ј UTRs. Recently, evidence was presented indicating that the P1, P2, and CP genes play a cis-acting role in the replication of RNAs 1, 2, and 3, respectively (32,35). Our results indicate that minus-strand RNA synthesis in vitro does not require cis-acting sequences at internal positions of the AMV plus-strand RNAs, as has been reported for bacteriophage Q␤ RNA (14).…”
Section: Discussionsupporting
confidence: 82%
“…Deletion mutants of RNAs 1 and 2 (see Fig. 4) were constructed by transferring relevant restriction fragments, encompassing the deletions, from previously engineered deletion mutants in the corresponding 35S/cDNA constructs (35). cDNA 1 deletion mutants are as follows (with the deleted nucleotides in brackets): 1-⌬PS (205 to 357), 1-⌬PAp (205 to 1107), 1-⌬HEn (1118 to 1424), 1-⌬EnBs (1425 to 2059), 1-⌬BsXb (2064 to 2464), and 1-⌬XbD (2469 to 3517).…”
Section: Methodsmentioning
confidence: 99%
“…The observation of transcriptional slippage in potyviruses opens the possibility of transcriptional slippage for gene expression in other positive-sense RNA viruses, particularly those with polyadenylated genomes [whose polymerases may therefore have evolved stuttering mechanisms to maintain poly(A) tail length] and where replication is linked to translation through certain genomic regions that become inaccessible in edited transcripts. Relevant to this, many positive-sense RNA virus genomes are preferentially replicated in cis 45 - 47 , 48 .…”
Section: Resultsmentioning
confidence: 99%
“…Similarly, defective RNAs can be amplified efficiently by the viral replicase provided by helper viruses. In contrast, the cis-preferential function of the virus-encoded proteins or a coupling between translation and replication has been reported for many positive-strand RNA viruses, including Alfalfa mosaic virus (AMV) (37,57), Bovine coronavirus (5), Poliovirus (14,21,39), Turnip crinkle virus (TCV) (62), Tobacco etch virus (31,49), Tobacco mosaic virus (TMV) (29), TBSV (42), Turnip yellow mosaic virus (TYMV) (60), and Red clover necrotic mosaic virus (RCNMV) (40). The coupling between translation and RNA replication appears to play an important role in virus infection.…”
mentioning
confidence: 99%