Accumulation of Ammonia in Plants Treated with Bialaphos

Tachibana, Kunitaka; Watanabe, Tomoaki; Sekizawa, Yasuharu; Takematsu, Tetsuo

doi:10.1584/jpestics.11.33

Cited by 130 publications

(58 citation statements)

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“…Confirmed by PCR, Southern or Western analysis and inhibits glutamine synthetase (Tachibana and Kaneko 1986;Tachibana et al 1986a) resulting in slow cell death due to ammonia accumulation (Tachibana et al 1986b). However, the concentrations of bialaphos commonly used during the callus induction stage (1.0-3.0 mg/l) do not effectively inhibit non-transformed calli, at least within 2-4 weeks of culture (Weeks et al 1993;Altpeter et al 1996), which results in numerous non-transformed plants.…”

Section: Resultsmentioning

confidence: 99%

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

Zhang

Rybczyński

Langenberg³

et al. 2000

Plant Cell Reports

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Langenberg, W.G.; Mitra, Amitava; and French, Roy C., "An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration" (2000). Abstract A method for producing large numbers of transgenic wheat plants has been developed. With this approach, an average of 9.7% of immature embryo explants were transformed and generated multiple selffertile, independently transformed plants. No untransformed plants, or escapes, were regenerated. This transformation procedure uses morphogenic calli derived from scutellum tissue of immature embryos of Triticum aestivum cv. Bobwhite co-bombarded with separate plasmids carrying a selectable marker gene (bar) and a gene of interest, respectively. Transformed wheat calli with a vigorous growth phenotype were obtained by extended culture on media containing 5.0 mg/l bialaphos. These calli retained morphogenic potential and were competent for plant regeneration for as long as 11 months. The bar gene and the gene of interest were co-expressed in T0 progeny plants. This wheat transformation protocol may facilitate quantitative production of multiple transgenic plants and significantly reduce the cost and labor otherwise required for screening out untransformed escapes.

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Section: Resultsmentioning

confidence: 99%

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

Zhang

Rybczyński

Langenberg³

et al. 2000

Plant Cell Reports

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show abstract

“…The different effects of hygromycin B and kanamycin on hypocotyl elongation may be related to the target of these antibiotics. Hygromycin B inhibits cytosolic protein synthesis (Cabanas MJ 1978), whereas kanamycin inhibit plastid protein synthesis (Gray JC 1984) and glutamine synthase activity (Tachibana K 1986), respectively. This suggests that cytosolic, but not plastid, protein synthesis is required for hypocotyl elongation in the dark.…”

Section: Discussionmentioning

confidence: 99%

A Rapid and Simple Method for Brassica Napus Floral-Dip Transformation and Selection of Transgenic Plantlets

Li¹,

Tan²,

Zhu³

et al. 2010

IJB

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The floral-dip method of transformation by immersion of inflorescences in a suspension of Agrobacterium was applied in the Brassica napus transformation, but it involves a number of relatively time-consuming and laborious steps, including manipulating an Agrobacterium tumefaciens culture and aseptic procedures for the selection of plant lines harboring antibiotic-selection markers. It calls for a long time to prepare the buffered media. To circumvent these bottlenecks, we have developed a rapid and simple method. We find that Brassica napus can be transformed by dipping directly into an Agrobacterium tumefaciens culture supplemented with surfactant, eliminating the need for media exchange to a buffered solution. We report a method of transformant selection by soaking seeds with antibiotic. These methods save time and money, and reduce the possibility of contamination.

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“…Ele compete pelos locais em que o glutamato se liga à glutamina sintetase onde, uma vez que o glufosinato tem sucesso, a ligação se torna irreversível (Logusch et al, 1991). A irreversível ligação entre o amônio glufosinato e a glutamina sintetase resulta em rápida acumulação, em níveis tóxicos, de amônia dentro da célula (Kishore & Shah, 1988;Tachibana et al, 1986a).…”

Section: Fixação Do Nitrogênio E Mecanismo De Ação Do Amônio Glufosinatounclassified

Aspectos do mecanismo de ação do amônio glufosinato: culturas resistentes e resistência de plantas daninhas

2014

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Resumo -Com o advento de culturas geneticamente modificadas para a tolerância ao herbicida não-seletivo amônio glufosinato no Brasil, associado à sua importância como herbicida alternativo às áreas infestadas por plantas daninhas resistentes ao glyphosate, o conhecimento de certos aspectos sobre seu mecanismo de ação, particularidades da molécula e manejo consciente tornamse necessários. Nesse sentido, a presente revisão de literatura foi realizada na tentativa de abranger, à luz do atual conhecimento, os conceitos sobre a bioquímica envolvida no mecanismo de ação desse herbicida, sua dinâmica de entrada na célula vegetal, sua utilização em culturas geneticamente modificadas e seu uso consciente, com a intensão de prolongar ao máximo possível essa ferramenta do sistema de produção de alimentos. Palavras-chaves: gene pat, gene bar, planta daninha resistente, OGM, mecanismo de ação Abstract -With the release of genetically modified organisms for tolerance of the non-selective herbicide ammonium-glufosinate in Brazil, along with its importance as an alternative herbicide to glyphosate-resistant weed, the knowledge of certain aspects related to its mode of action, chemical features and rational management become necessary. In this context, the present literature review was idealized in an attempt to cover, in the light of the current knowledge, some concepts about the biochemistry related to the mode of action of this herbicide, its uptake dynamics, its usage in GM plants and its rational management, with the purpose of making it last as long as possible in the agriculture systems. Keywords: herbicide-resistant weed, pat gene, bar gene, GMO, mode of action IntroduçãoA assimilação do nitrogênio constitui, exceto quando comparado à assimilação do CO2, a função mais importante da célula de uma planta. Nesse contexto, a glutamina sintetase é uma enzima chave na assimilação de amônia nas 1 Recebido para publicação em 13/12/2014 e aceito em 25/05/2015.

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Accumulation of Ammonia in Plants Treated with Bialaphos

Cited by 130 publications

References 0 publications

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

An efficient wheat transformation procedure: transformed calli with long-term morphogenic potential for plant regeneration

A Rapid and Simple Method for Brassica Napus Floral-Dip Transformation and Selection of Transgenic Plantlets

Aspectos do mecanismo de ação do amônio glufosinato: culturas resistentes e resistência de plantas daninhas

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