Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer

Hiraide, Takanori; Ikegami, Koji; Sakaguchi, Takanori; Morita, Yoshifumi; Hayasaka, Takahiro; Nio, Masaki; Waki, Michihiko; Sugiyama, Eiji; Shinriki, Satoru; Takeda, Makoto; Shibasaki, Yasushi; Miyazaki, Shôzo; Kikuchi, Hirotoshi; Okuyama, Hiroaki; Inoue, Masahiro; Setou, Mitsutoshi; Konno, Hiroyuki

doi:10.1038/srep29935

Cited by 47 publications

(43 citation statements)

References 47 publications

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“…In addition to proteins and drugs, MALDI MSI is also able to analyze other biological molecules such as lipids and glycans . Hiraide et al.…”

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 99%

“…Hiraide et al. (2016) used MALDI MSI to characterize the lipid profile of primary tissue derived colon cancer MCTS and identified high expression of a lipid mass on the periphery of the MCTS. Laser capture microdissection (LCD) of the outer regions of the MCTS followed by LC‐MS/MS was used to identify this mass as phosphatidylinositol (18:0/20:4).…”

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 99%

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Mass Spectrometry Analyses of Multicellular Tumor Spheroids

Acland

Mittal

Lokman

et al. 2018

Proteomics Clinical Apps

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Multicellular tumor spheroids (MCTS) are a powerful biological in vitro model, which closely mimics the 3D structure of primary avascularized tumors. Mass spectrometry (MS) has established itself as a powerful analytical tool, not only to better understand and describe the complex structure of MCTS, but also to monitor their response to cancer therapeutics. The first part of this review focuses on traditional mass spectrometry approaches with an emphasis on elucidating the molecular characteristics of these structures. Then the mass spectrometry imaging (MSI) approaches used to obtain spatially defined information from MCTS is described. Finally the analysis of primary spheroids, such as those present in ovarian cancer, and the great potential that mass spectrometry analysis of these structures has for improved understanding of cancer progression and for personalized in vitro therapeutic testing is discussed.

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“…In addition to proteins and drugs, MALDI MSI is also able to analyze other biological molecules such as lipids and glycans . Hiraide et al.…”

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 99%

Section: Analysis Techniques For Investigating Mctsmentioning

confidence: 99%

Mass Spectrometry Analyses of Multicellular Tumor Spheroids

Acland

Mittal

Lokman

et al. 2018

Proteomics Clinical Apps

View full text Add to dashboard Cite

show abstract

“…MALDI-IMS has been applied to various tissues and diseases such as cancer (Hiraide et al, 2016) and heart disease (Angel et al, 2016) and, more recently, in the brain to understand the lipid changes in alcohol-related brain damage (de la Monte et al, 2018), AD (Gónzalez de San Román, Manuel, Giralt, Ferrer, & Rodríguez-Puertas, 2017;Mendis, Grey, Faull, & Curtis, 2016), or the lipid cytoarchitecture of regions such as the subventricular zone (Hunter, Demarais, Faull, Grey, & Curtis, 2018). We have previously described a robust methodology for maximizing peptide ionization from formalin-fixed frozen human brain tissue (O'Rourke et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Higher Mass Accuracy MALDI‐TOF/TOF Lipid Imaging of Human Brain Tissue in Alzheimer's Disease

O’Rourke

Smith

Sutherland

et al. 2019

CP Molecular Biology

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Matrix‐assisted laser desorption/ionization imaging mass spectrometry (MALDI‐IMS) is a well‐established technique for elucidating the location and relative abundance of a range of biomolecules. More recently, research into this technique has shifted from simple discovery and demonstration of utility to application in biomedical research. Here, we describe a protocol utilizing MALDI‐IMS for the spatial mapping of lipids in brain tissue from normal human brains and brains from patients with Alzheimer's disease, in the context of Alzheimer's disease. Improved accuracy calibration of the instrument from the tissue surface is emphasized, as this allows for significantly improved mass determination in time of flight (TOF)‐based instruments enabling more confident preliminary lipid identification. This improved initial result allows MALDI‐IMS data to be complemented with additional instrumentation, such as liquid chromatography mass spectrometry workflows or specialized non‐TOF systems such as Fourier transform cyclotron resonance instruments. This method is not limited to human tissue and can be applied to virtually any lipid‐rich formalin‐fixed tissue. © 2019 by John Wiley & Sons, Inc.

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“…In colorectal primary cancer cells cultured in 3D systems (also known as cancer tissue‐originated spheroids, CTOSs) the amount of total PI with 18:0/20:4 acyl chains as evaluated by imaging mass spectrometry was found to be similar to those reported for healthy liver and brain tissue . However, the 18:0/20:4 PI molecular species was not uniformly distributed in the CTOSs, rather a preferential localization of these species at the CTOSs surface was observed as well as at the cancer cells/stromal interface, which led to the proposition that this localization was important for colorectal cancer progression . Moreover, in human retinal pigment epithelial cells PI(4,5)P2 and PI(3)P levels and localization where altered when the acyl chain profile was changed by knock down of LYCAT1 .…”

Section: Putative Functional Role For 18:0/20:4 Acyl Chains In Pi/pipnmentioning

confidence: 84%

Specificity of Acyl Chain Composition of Phosphatidylinositols

Bozelli

Epand

2019

Proteomics

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Phosphatidylinositol (PI) lipids have a predominance of a single molecular species present through the organism. In healthy mammals this molecular species is 1‐stearoyl‐2‐arachidonoyl (18:0/20:4) PI. Although the importance of PI lipids for cell physiology has long been appreciated, less is known about the biological role of enriching PI lipids with 18:0/20:4 acyl chains. In conditions with dysfunctional lipid metabolism, the predominance of 18:0/20:4 acyl chains is lost. Recently, molecular mechanisms underpinning the enrichment or alteration of these acyl chains in PI lipids have begun to emerge. In the majority of the cases a common feature is the presence of enzymes bearing substrate acyl chain specificity. However, in cancer cells, it has been shown that one (not the only) of the mechanisms responsible for the loss in this acyl chain enrichment is mutation on the transcription factor p53 gene, which is one of the most highly mutated genes in cancers. There is a compelling need for a global picture of the specificity of the acyl chain composition of PIs. This can be possible once high‐resolution spatio‐temporal information is gathered in a cellular context; which can ultimately lead to potential novel targets to combat conditions with altered PI acyl chain profiles.

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Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer

Cited by 47 publications

References 47 publications

Mass Spectrometry Analyses of Multicellular Tumor Spheroids

Mass Spectrometry Analyses of Multicellular Tumor Spheroids

Higher Mass Accuracy MALDI‐TOF/TOF Lipid Imaging of Human Brain Tissue in Alzheimer's Disease

Specificity of Acyl Chain Composition of Phosphatidylinositols

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