Accumulation of cells containing factor XIII subunit a around the foci of intense fibrosis in human epulides

Toida, Makoto; Oka, Nobumitsu; Takami, Taro; Ádány, Róza

doi:10.1007/bf00173709

Cited by 4 publications

(6 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A similar increase in the number of FXIII A + cells has been observed in our recent studies, in which a huge number of FXIII A--cells could be detected in several kinds of human fibrosing lesions (Toida et al, 1990b(Toida et al, , 1991(Toida et al, , 1995. The present findings suggest that FXIII A § cells, which can release FXIII A into the extracellular matrix, may play an important role in the processes of stromal desmoplasia and/or matrix reorganization in buccal squamous-cell carcinoma.…”

Section: Discussionsupporting

confidence: 66%

See 1 more Smart Citation

Characterization of cells containing factor XIII subunit a in benign and malignant buccal lesions

Toida¹,

Okumura

Win

et al. 1995

Histochem J

Self Cite

View full text Add to dashboard Cite

In the present study, the distribution pattern and characteristics of cells containing Factor XIII subunit a (FXIII A) have been studied in benign and malignant lesions of human buccal mucosa. Tissues from four irritation fibromas and three squamous cell carcinomas were studied by means of double immunofluorescent staining techniques in which the detection of FXIII A was combined with a reaction with CD14 (recognizing a monocyte/macrophage differentiation marker antigen), Mac 387 (reacting with a special subset of macrophages), anti-HLA-DR, Ki-M7 (labelling phagocytosing macrophages) or Ki-67 (visualizing a nuclear antigen associated with cell proliferation) monoclonal antibodies. FXIII A was detected in cells of the connective tissue stroma in both benign and malignant buccal lesions. The number of these FXIII A-reactive cells (FXIII A+ cells) increased considerably in the tumour tissues, in particular in those surrounding tumour cell clusters. FXIII A+ cells scattered in the fibromatous tissues were spindle-shaped, whereas in the tumour stroma, large stellate cells predominated, and round cells were likewise labelled around blood vessels. FXIII A+ cells were labelled with CD14 and Ki-M7 in both fibromatous and tumoural buccal mucosa; however, they failed to show any reaction with Ki-67. FXIII A+ cells accumulated in the tumour stroma reacted for HLA-DR as well. These results indicate that in both the benign and malignant buccal lesions FXIII A is contained in a subpopulation of tissue macrophages, which represents a monocyte-derived (CD14+) and phagocytosing (Ki-M7+) cell population. The accumulation of the FXIII A+ cells in the tumour stroma is believed to be a result of direct migration from the circulating blood.(ABSTRACT TRUNCATED AT 250 WORDS)

show abstract

Section: Discussionsupporting

confidence: 66%

“…All of these monoclonal antibodies were raised in mice against human antigens. The characteristics of these antibodies are summarized elsewhere (Toida et al, 1995).…”

Section: Double Immunofluorescence Reactionsmentioning

confidence: 99%

Characterization of cells containing factor XIII subunit a in benign and malignant buccal lesions

Toida¹,

Okumura

Win

et al. 1995

Histochem J

Self Cite

View full text Add to dashboard Cite

show abstract

“…The a-subunit of FXIIIa belongs to a family consisting of several genes that encode for enzymes with a common active-site structure, well conserved among species (17). Accumulating evidence points to an additional role for FXIIIa in prolifera-tion of connective tissues via its expression by invading macrophages (18,19). In this study, we discovered some characteristics of the a-subunit of FXIIIa in fish plasma that are distinct from those of its mammalian counterpart.…”

mentioning

confidence: 76%

“…In addition to its participation in blood coagulation, FXIIIa appears to be the extracellular TGase involved in wound healing (16,19,20). The mammalian PNS tissue, according to the present study, seems to respond very efficiently to injury, as shown for example by the fact that FXIIIa becomes fully active (i.e., could not be further activated by the addition of thrombin) as a result of sciatic nerve excision.…”

Section: Discussionmentioning

confidence: 99%

Factor XIIIa as a nerve‐associated transglutaminase

et al. 1998

View full text Add to dashboard Cite

Recent findings have led to changes in the traditional concept of nerve recovery, including the realization that injured nerves, like any other injured tissue, need the assistance of blood-derived cells and factors in order to heal. We show that factor XIIIa (FXIIIa, the potentially active a2subunit of factor XIII), an enzyme that participates in blood coagulation by stabilizing the fibrin clot, is also active in the nervous system where it may play a key role in the healing of injured tissue. We demonstrate that the plasma, macrophages and nerves of fish contain a 55 kDa form of transglutaminase that cross-reacts immunologically with the a-subunit of FXIII in mammals (80 kDa). The fish enzyme in the plasma, unlike its mammalian counterpart, is active, pointing to a difference in control of the coagulation pathway in the two species. Analysis of FXIIIa expression in mammalian neural tissues and their response to injury revealed high levels of the enzyme in media conditioned by peripheral nerves as compared with medium conditioned by nerves of the central nervous system. Furthermore, similarity was observed in the postinjury behavior of FXIIIa in regenerating nerve tissues (peripheral nervous system of mammals and the central nervous system of fish). We suggest that the postinjury level of factor XIIIa in the nervous system may be related to the tissue's regenerative capacity, and that FXIIIa may therefore be a link underlying a possible association between the processes of blood coagulation and nerve healing.

show abstract

“…In the present study we have shown that TGF-β co-localizes with the blood coagulation and wound healing protein XIII α in fibrotic lesions of carcinoid heart disease. Although the main function of XIII α is the cross-linking of fibrin molecules, it also takes part in wound healing and, as previous research indicated, in fibrotic processes [20, 21]. In this context it is noteworthy that fibrinogen and fibrin have been previously identified in carcinoid plaques [22].…”

Section: Discussionmentioning

confidence: 99%

Demonstration of TGF-? and XIII? in Endocardial Biopsies of Carcinoid Heart Disease Patients: an Immunofluorescence Study

et al. 2011

View full text Add to dashboard Cite

BackgroundSerotonin and other vasoactive substances play a critical role in the development of carcinoid heart disease, but the exact etiology of the illness is still unknown.MethodsBy using immunofluorescence microscopy, we investigated the expression of transforming growth factor-β (TGF-β) and the presence of fibrin-stabilizing factor (XIIIα) in endomyocardial biopsy specimens of patients with carcinoid heart disease. In addition, the tissue integrity of the specimens was studied by staining for laminin.ResultsBoth TGF-β and XIIIα co-localized in the endocardium beneath carcinoid plaques: while TGF-β was found within myocytes, XIIIα was detected on the surface of cells in fibrotic lesions stretching out into the tissue. Laminin staining revealed that the integrity of the endocardium was dissolved and that the tissue consisted of hypertrophic and hypotrophic myocytes.ConclusionsThe results suggest that the presence of TGF-β and XIIIα in carcinoid heart lesions indicates that endocardial damage induced by serotonin and other vasoactive substances gives rise to an overshooting wound healing process.

show abstract

Accumulation of cells containing factor XIII subunit a around the foci of intense fibrosis in human epulides

Cited by 4 publications

References 35 publications

Characterization of cells containing factor XIII subunit a in benign and malignant buccal lesions

Characterization of cells containing factor XIII subunit a in benign and malignant buccal lesions

Factor XIIIa as a nerve‐associated transglutaminase

Demonstration of TGF-? and XIII? in Endocardial Biopsies of Carcinoid Heart Disease Patients: an Immunofluorescence Study

Contact Info

Product

Resources

About