Accumulation of chromotrope 2R positive cells in equine endometrium during early pregnancy and expression of transforming growth factor- 2 (TGF- 2)

Lea, Richard G.; Stewart, Francesca; Allen, William R.; Ohno, Isao; Clark, D A

doi:10.1530/jrf.0.1030339

Cited by 11 publications

(3 citation statements)

References 9 publications

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“…A carbol chromotrope stain was used to identify guinea pig eosinophils, with an adaptation of the method described by Lendrum (17). This stain is concentrated in the granules of eosinophils due to their avidity for acid dyes and has been shown by several investigators to be specific for eosinophils (15)(16)(17)25). Briefly, paraffin slides were dewaxed in xylenes, rehydrated through graded alcohols, and washed in PBS.…”

Section: Measurement Of Histamine-induced Contractile Responsesmentioning

confidence: 99%

Effects of IL-13 on airway responses in the guinea pig

Morse

Sypek²,

Donaldson³

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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.-Levels of interleukin (IL)-13 are increased in asthmatic airways. IL-13 has been shown to be necessary and sufficient for allergen-induced airway hyperresponsiveness and increased inflammatory cell counts in bronchoalveolar lavage (BAL) fluid in a murine model of asthma but is thought to protect against airway inflammation when low doses are provided to the guinea pig lung. To determine the role of IL-13 in the guinea pig, we studied the effects of a 360-g/kg dose of nebulized IL-13 in naive animals and of IL-13 abrogation after airway challenge of sensitized animals. Nebulized IL-13 significantly decreased the dose of histamine required to double baseline respiratory system resistance (ED 100, 22 Ϯ 3 vs. 13 Ϯ 2 nmol/kg; P Ͻ 0.05) and was associated with recovery of significantly greater numbers of macrophages, lymphocytes, eosinophils, and neutrophils in BAL fluid. Guinea pigs pretreated with a fusion protein that binds IL-13 [soluble IL-13 receptor ␣2 (sIL-13R␣2)] were protected from developing antigen-induced airway hyperresponsiveness (ED100, 210 Ϯ 50 vs. 20 Ϯ 10 nmol/kg; P Ͻ0.01). sIL-13R␣2 (2 doses of 20 mg/kg) significantly reduced the histological grade of allergen-induced lung eosinophil accumulation, whereas the effects of two doses of 10 mg/kg were not significant. These findings demonstrate that the tissue levels of IL-13 induced by allergen challenge of sensitized animals induce airway hyperresponsiveness and inflammation and that IL-13 is required for the expression of allergen-induced airway hyperresponsiveness in the guinea pig ovalbumin model. interleukin-13; eosinophil; inflammation; airway hyperresponsiveness; ovalbumin IN THE PAST DECADE, ASTHMA has been increasing in prevalence and is now thought to affect 15 million Americans (13). Advances have been made in our understanding of the immunobiology of sensitization (1); the role of interleukin (IL)-4, IL-5, and IL-13 in the development of airway hyperresponsiveness; the recruitment of inflammatory cells to the airway; and the augmented mucus production that follows allergen challenge in a murine system. Strong circumstantial evidence indicates that Th2 lymphocytes and the cytokines they produce are important in the pathogenesis of asthma (3, 5, 6, 10-12, 20, 26).In the mouse, Th2 lymphocytes selectively develop and expand in a process that depends on IL-4 (1, 5). Studies comparing allergen-induced airway hyperresponsiveness in genetically altered receptor-or liganddeficient mice have demonstrated a greater dependence on the IL-4 receptor ␣-chain than on 14). In addition to the IL-4 avid form of the heterodimeric receptor (IL-4 receptor ␣-chain/common ␥-chain), the IL-4 receptor ␣-chain can combine with IL-13 receptor ␣1 and efficiently bind 12,24); this realization in murine biology led to speculation that IL-13 was the mediator responsible for allergen-induced pulmonary inflammatory cell recruitment and airway hyperresponsiveness in the mouse model (2). The availability of the fusion protein soluble sIL-13 receptor ␣2 (sIL-13R␣2), which se...

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Section: Measurement Of Histamine-induced Contractile Responsesmentioning

confidence: 99%

Effects of IL-13 on airway responses in the guinea pig

Morse

Sypek²,

Donaldson³

et al. 2002

American Journal of Physiology-Lung Cellular and Molecular Phys

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“…It is also at about this time that the endometrial cups are reaching the end of their lifespan, and eosinophils are abundant in the cellular infiltrate around the dying cup cells (Grunig et al 1995). Eosinophils surrounding the mature endometrial cup express TGF-b2 mRNA (Lea et al 1995). In normal post-partum Thoroughbred mares, the percentage of eosinophils present on uterine cytology increased markedly on Days 5 and 6 after foaling (Saltiel et al 1987).…”

Section: Fluid and Tissue Distribution Of The Eosinophil In Healthy Hmentioning

confidence: 99%

Eosinophils of the horse: Part I: Development, distribution, structure and biochemical mediators

Brosnahan

2018

Equine Veterinary Education

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Summary Eosinophils are becoming the target of increasing research interest as recent studies suggest that their role in immune homoeostasis and the immune response to disease is far more complex than previously understood. Historically, the horse eosinophil has been used to study basic eosinophil biology because of the considerable volume of blood required to obtain enough viable cells for reliable, repeatable experiments. This resulted in a large but disseminated body of literature pertaining to the structure and function of the horse eosinophil. More recently, equine clinicians have produced case reports and clinical studies in an effort to define the role of the eosinophil in diseases of the horse. A thorough review of the equine eosinophil incorporating both bench research and clinical reports does not exist. The objective of this two‐part review is to fill this need by integrating the basic science and clinical research into a comprehensive body of work on what is known specifically about the horse eosinophil, and its role in equine health and disease. Part I summarises the development and tissue distribution of eosinophils in the normal horse, and presents what is known about the cell structure, migration and biochemical mediators of the horse eosinophil. Part II reviews the role of the eosinophil in diseases of the horse, and concludes with a summary of knowledge gaps and open research questions to benefit both those who wish to use the equine eosinophil as a model for basic science research, and those whose primary interest lies with diseases of horse.

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“…TGF32-related suppressor activity and ya T cells are present in the decidua of other murine strain mating combinations, but there are some differences in the frequency of yb T cells in the decidua of abortion-prone matings from the time of implantation [1][2][3]21]. In pregnant horses, our TGF[2 probe appears to bind to eosinophils, a phenomenon not seen in the mouse, and there has been no formal analysis of the release of bioactive TGF[s [43]. In the pregnant sheep, y IEL express an activation marker [44], and a similar phenomenon has been reported for lymphocytes isolated from human pregnancy decidua [45].…”

mentioning

confidence: 99%

Decidua-Associated Suppressor Cells in Abortion-Prone DBA/2-Mated CBA/J Mice that Release Bioactive Transforming Growth Factor β2-related Immunosuppressive Molecules Express a Bone Marrow-Derived Natural Suppressor Cell Marker and γδ8 T-Cell Receptor1

Clark¹,

Merali²,

Hoskin³

et al. 1997

Biology of Reproduction

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The decidua of allopregnant mice contains a novel population of Thy1 Lyt1 CD4 CD8 asialoGM1- non-B small lymphocytic suppressor cells that release transforming growth factor (TGF) SS2-related suppressor molecules. The "null" phenotype of this cell population is similar to some bone marrow-derived natural suppressor cell (NSC) populations, and the latter may release TGF(beta)s. We now report that the TGF beta2-producing suppressor cells in the uterine decidua of DBA/2-mated CBA/J female mice-linked to prevention of abortions-are inactivated effectively by 1E5/B5.1 but not by 2C1.1 rat monoclonal antibodies to murine pregnancy-associated splenic NSC in the presence of complement. Immunostaining of a subpopulation of cells in decidua with 1E5/B5.1 but not with 2C1.1 was shown by flow cytometry. Release of suppressor factor was also abrogated by 1E5/B5.1 + complement but not by 2C1.1 + complement, and the suppressor factor was specifically neutralized by anti-TGF beta2 and not by anti-TGF beta3. Splenic pregnancy NSC are susceptible to 2C1.1, produce TGF beta1, and express CD3 and alpha beta T-cell receptor (TcR) chains. Release of suppressor factor by the decidual NSC was abrogated by treatment with anti-CD3 (145 2C11) and anti-TcR gamma delta (GL4) monoclonal antibodies + complement, but not by anti-TcR alpha beta (H57) + complement; and cells sorted using anti-TcR gamma delta (GL3) released suppressive activity in vitro. Slightly more suppressive activity was released by implantation-site decidua where there was no epithelium than from epithelialized inter-implantation-site decidua; no significant activity was released from placental tissue, but combining implantation-site tissue with placental tissue led to release of enhanced levels of immunosuppressive activity. There appear to be subtypes of bone marrow-derived TcR+ NSC with different phenotypes and tissue localization patterns in pregnancy. The previously reported dependence of decidual NSC activity on the presence of soluble signals from fetal trophoblast may be explainable by the ability of cells bearing TcR gamma delta to recognize and react to placental trophoblast cell antigen.

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Accumulation of chromotrope 2R positive cells in equine endometrium during early pregnancy and expression of transforming growth factor- 2 (TGF- 2)

Cited by 11 publications

References 9 publications

Effects of IL-13 on airway responses in the guinea pig

Effects of IL-13 on airway responses in the guinea pig

Eosinophils of the horse: Part I: Development, distribution, structure and biochemical mediators

Decidua-Associated Suppressor Cells in Abortion-Prone DBA/2-Mated CBA/J Mice that Release Bioactive Transforming Growth Factor β2-related Immunosuppressive Molecules Express a Bone Marrow-Derived Natural Suppressor Cell Marker and γδ8 T-Cell Receptor1

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