Accumulation of Galactinol and ABA Is Involved in Exogenous EBR-Induced Drought Tolerance in Tea Plants

Zhang, Yongheng; Xiao, Yezi; Zhang, Yingao; Dong, Yanjun; Liu, Yingqing; Liu, Lu; Wan, Shungang; He, Jingyuan; Yu, Youben

doi:10.1021/acs.jafc.2c04892

Cited by 14 publications

(6 citation statements)

References 67 publications

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“…Previous research suggested that there exists a complex network between brassinosteroids and other hormones in the regulation of drought resistance [ 25 , 32 , 33 ]. The results showed that EBR application could increase the endogenous ABA contents under drought stress, which may be due to EBR application activating the expression of ABA biosynthesis genes [ 34 , 35 ]. This can also be supported by Yuan et al [ 36 ] , who reported that applications of exogenous EBR significantly increased ABA content in tomatoes under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Exogenous 24-Epibrassinolide Enhanced Drought Tolerance and Promoted BRASSINOSTEROID-INSENSITIVE2 Expression of Quinoa

Zhou,

You,

Wang

et al. 2024

Plants

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Brassinosteroids (BRs) are involved in the regulation of biotic and abiotic stresses in plants. The molecular mechanisms of BRs that alleviate the drought stress in quinoa have rarely been reported. Here, quinoa seedlings were treated with 24-epibrassinolide (EBR) and we transiently transferred CqBIN2 to the quinoa seedlings’ leaves using VIGS technology to analyze the molecular mechanism of the BR mitigation drought stress. The results showed that EBR treatment significantly increased the root growth parameters, the antioxidant enzyme activities, and the osmolyte content, resulting in a decrease in the H2O2, O2∙−, and malondialdehyde content in quinoa. A transcriptome analysis identified 8124, 2761, and 5448 differentially expressed genes (DEGs) among CK and Drought, CK and EBR + Drought, and Drought and EBR + Drought groups. WGCNA divided these DEGs into 19 modules in which these characterized genes collectively contributed significantly to drought stress. In addition, the EBR application also up-regulated the transcript levels of CqBIN2 and proline biosynthesis genes. Silenced CqBIN2 by VIGS could reduce the drought tolerance, survival rate, and proline content in quinoa seedlings. These findings not only revealed that exogenous BRs enhance drought tolerance, but also provided insight into the novel functions of CqBIN2 involved in regulating drought tolerance in plants.

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Section: Discussionmentioning

confidence: 99%

Exogenous 24-Epibrassinolide Enhanced Drought Tolerance and Promoted BRASSINOSTEROID-INSENSITIVE2 Expression of Quinoa

Zhou,

You,

Wang

et al. 2024

Plants

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“…Salinity is a major abiotic stress factor that seriously affects crop growth and yield, causing significant economic losses and threatening global food security [ 35 , 36 ]. At present, 23.02 billion hectares of farmland worldwide can be used for food production.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsDUF6 increases salt stress tolerance in rice

Ma,

Zhang,

2024

BMC Plant Biol

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Background Soil salinity is one of the primary environmental stresses faced in rice production. When plants are exposed to salt stress, a series of cellular balances will be disrupted. Dufulin is an immune-induced antiviral agent used in plants. The DUF gene family influences plant response to abiotic stress, and the functional role of OsDUF6(ABA98726.1) in rice response to salt stress is being investigated here. Results Based on the transcriptome analysis of Dufulin treatment in inducing salt tolerance in rice, we selected the OsDUF6 protein located on the cell membrane and studied its molecular function by overexpressing OsDUF6. Salt-induced decreases in root, stem, and leaf length and increased leaf yellowing rate and Na+ concentration in the wild-type plant were mitigated in the overexpressed lines. OsDUF6 overexpression increased the enzymatic antioxidant activities of superoxide dismutase, peroxidase, catalase, and phenylalanine ammonia-lyase. OsDUF6 also played a positive role in Na+ transport as reflected by the increased growth of a salt-sensitive yeast mutant complemented with OsDUF6 in the presence of salt stress. In addition, Reverse transcription quantitative PCR analysis confirmed that the overexpression of OsDUF6 significantly changed the expression level of other genes related to growth and stress tolerance. Conclusions Combined with previously published data, our results supported the observation that OsDUF6 is an important functional factor in Dufulin-induced promotion of salt stress tolerance in rice.

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“…The number of miR156 members in each species ranged from 1 to 47 (Figure 1A). To further investigate the evolutionary relationship between miR156s in the plants, we used Physcomitrella patens (3), Selaginella moellendorffii (4), Oryza Sativa (18), Glycine max (28), Vitis vinifera (9), Arabidopsis thaliana (13), Medicago truncatula (15), Nicotiana tabacum (10), Brassica napus (7), Picea abies (27), and Camellia sinensis (47) to construct a phylogenetic tree (Figure S1). The phylogenetic tree was divided into three clades.…”

Section: Identification and Analysis Of Mir156 In Plantsmentioning

confidence: 99%

“…Recent studies have shown that (Z)-3-hexanol and eugenol can enhance the drought tolerance of tea plants by mediating glucosylation [ 6 ]. An exogenous application of 24-epibrassinolide can improve the drought resistance of tea plants by promoting the expression of genes involved in the biosynthesis of galactinol and abscisic acid [ 7 ]. In addition, several genes such as CsUGT71A59 , CsGSTU8 , and CsSnRK2.5 have shown the ability to enhance drought tolerance by regulating the accumulation of reactive oxygen species and altering abscisic acid homeostasis [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Identification and Validation of the miR156 Family Involved in Drought Responses and Tolerance in Tea Plants (Camellia sinensis (L.) O. Kuntze)

Wen,

Zhou,

Tian

et al. 2024

Plants

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The microRNA156 (miR156) family, one of the first miRNA families discovered in plants, plays various important roles in plant growth and resistance to various abiotic stresses. Previously, miR156s were shown to respond to drought stress, but miR156s in tea plants (Camellia sinensis (L.) O. Kuntze) have not been comprehensively identified and analyzed. Herein, we identify 47 mature sequences and 28 precursor sequences in tea plants. Our evolutionary analysis and multiple sequence alignment revealed that csn-miR156s were highly conserved during evolution and that the rates of the csn-miR156 members’ evolution were different. The precursor sequences formed typical and stable stem–loop structures. The prediction of cis-acting elements in the CsMIR156s promoter region showed that the CsMIR156s had diverse cis-acting elements; of these, 12 CsMIR156s were found to be drought-responsive elements. The results of reverse transcription quantitative PCR (RT-qPCR) testing showed that csn-miR156 family members respond to drought and demonstrate different expression patterns under the conditions of drought stress. This suggests that csn-miR156 family members may be significantly involved in the response of tea plants to drought stress. Csn-miR156f-2-5p knockdown significantly reduced the Fv/Fm value and chlorophyll content and led to the accumulation of more-reactive oxygen species and proline compared with the control. The results of target gene prediction showed that csn-miR156f-2-5p targeted SQUAMOSA promoter binding protein-like (SPL) genes. Further analyses showed that CsSPL14 was targeted by csn-miR156f-2-5p, as confirmed through RT-qPCR, 5′ RLM-RACE, and antisense oligonucleotide validation. Our results demonstrate that csn-miR156f-2-5p and CsSPL14 are involved in drought response and represent a new strategy for increasing drought tolerance via the breeding of tea plants.

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Accumulation of Galactinol and ABA Is Involved in Exogenous EBR-Induced Drought Tolerance in Tea Plants

Cited by 14 publications

References 67 publications

Exogenous 24-Epibrassinolide Enhanced Drought Tolerance and Promoted BRASSINOSTEROID-INSENSITIVE2 Expression of Quinoa

Exogenous 24-Epibrassinolide Enhanced Drought Tolerance and Promoted BRASSINOSTEROID-INSENSITIVE2 Expression of Quinoa

Overexpression of OsDUF6 increases salt stress tolerance in rice

Identification and Validation of the miR156 Family Involved in Drought Responses and Tolerance in Tea Plants (Camellia sinensis (L.) O. Kuntze)

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