Accumulation of hyaluronan (hyaluronic acid) in myocardial interstitial tissue parallels development of transplantation edema in heart allografts in rats.

Abstract

By using biotin-labeled proteoglycan core protein, hyaluronan (hyaluronic acid; HA) was visualized in rat heart grafts at different times (2, 4, and 6 d) after transplantation. In normal, nontransplanted hearts HA was present in the adventitia of arteries and veins and in the myocardial interstitial tissue. An increased accumulation of HA was evident in the edematous interstitial tissue, infiltrated with lymphocytes, on day 4 after allogeneic transplantation, and was even more pronounced by day 6. No apparent increase in HA was seen in syngeneic grafts. Biochemical assay of HA in heart tissue demonstrated that the myocardial content of HA had increased 60% by day 2 after transplantation in allogeneic as well as syngeneic grafts, indicating that surgical trauma may induce some HA accumulation in heart grafts. The extractable amounts of HA declined during the following days in the syngeneic grafts, but increased progressively during the development of rejection in the allogeneic grafts, and increased on average three times by day 6. The relative water content also increased progressively during rejection of allogeneic grafts and correlated with the HA accumulation. The interstitial accumulation of HA, a glycosaminoglycan with unique water-binding qualities, is presumably implicated in the development of interstitial edema during rejection of heart grafts. (J. Clin. Invest. 1990. 85:668-673.) hyaluronic acid * heart graft * allograft -rejection * edema

Accumulation of hyaluronan and tissue edema in experimental myocardial infarction.

“…The hyaluronan distribution in the sham operated animals was identical to that previously stated (7). There was thus a positive staining for hyaluronan in the very narrow space between the individual myocytes, the endomysium, but also in the coarser connective tissue fasciculi of the perimysium around groups of muscle cells.…”

“…The procedure has been previously described (7). In Before staining for HA, the heart tissues were dehydrated in ethanol, cleared in xylene, embedded in paraffin and transverse sections, 5 Mm thick, were taken for staining.…”

“…In Before staining for HA, the heart tissues were dehydrated in ethanol, cleared in xylene, embedded in paraffin and transverse sections, 5 Mm thick, were taken for staining. The staining for HA was performed according to the principles given by Ripellino and associates (I 1) and as previously described by us (7).…”

Inflammatory cells and activation markers in BAL during acute rejection and infection in lung transplant recipients: a prospective, longitudinal study

“…Increased levels of HYA are found during acute rejection in kidney, liver and lung transplants, and is associated with the interstitial oedema seen in allograft tissue [9,10,22].…”

Dynamic role of hyaluronan (HYA) in connective tissue activation and inflammation

“…There was a progressive accumulation of HYA in the grafts starting as early as 2 days after transplantation and reaching very high levels when the rejection was completed and the graft became necrotic [29]. Six days after transplantation the concentration of HYA in the allograft had thus increased to more than three times that of the recipient's own heart.…”