Accumulation of hydroxy lipids in live fish infected with fish diseases

Tanaka, Ryusuke; Higo, Yoshikazu; Shibata, Toshiyuki; Suzuki, Nobutaka; Hatate, Hideo; Nagayama, Koki; Nakamura, Takashi

doi:10.1016/s0044-8486(01)00789-x

Cited by 17 publications

(18 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Determination of fatty acid composition was performed according to Tanaka et al [11]. The extracted lipid solutions of fish meat were transmethylated by sodium methoxide.…”

Section: Determination Of Fatty Acid Compositionmentioning

confidence: 99%

An Assay Method for Determining the Total Lipid Content of Fish Meat Using a 2‐Thiobarbituric Acid Reaction

Matsushita

Inoue

Tanaka

2010

J Americ Oil Chem Soc

Self Cite

View full text Add to dashboard Cite

A method for the determination of the total lipid content in fish meat was established using a 2-thiobarbituric acid (TBA) reaction, which had previously been used for the determination of lipid peroxides in animal tissues. In this method, an unspecific peroxidation of fish oils was created by omitting the addition of antioxidant to the reaction mixture during the TBA reaction, because fish meat is more sensitive to the TBA reaction due to its higher concentration of polyunsaturated fatty acids, especially docosahexaenoic acid and eicosapentaenoic acid, which can easily react artificially with TBA in the absence of an antioxidant in the assay system. As for a practical application of this method, we tried to optimize the assay procedures in the sampling, reaction, and detection steps of this method, and finally proposed a new standard procedure recommended for determining the total lipid content of fish using a TBA reaction. In order to confirm the accuracy of the new procedure, comparative evaluations for the lipid contents of commercially available fish, i.e., chub mackerel (Scomber japonicus) and saury (Cololabis saira) were made between the conventional procedure and the recommended TBA method. The lipid contents obtained by the two methods coincided well with high correlation. This method is relevant for total lipid content analysis of fish meat under restricted laboratory conditions.

show abstract

“…Determination of fatty acid composition was performed according to Tanaka et al [11]. The extracted lipid solutions of fish meat were transmethylated by sodium methoxide.…”

Section: Determination Of Fatty Acid Compositionmentioning

confidence: 99%

An Assay Method for Determining the Total Lipid Content of Fish Meat Using a 2‐Thiobarbituric Acid Reaction

Matsushita

Inoue

Tanaka

2010

J Americ Oil Chem Soc

Self Cite

View full text Add to dashboard Cite

show abstract

“…The coastal ecosystem is rapidly deteriorating from industry pollution and anthropogenic activities, and marine animals are the most susceptible to environmental changes and pollutants, such as salinity changes, heavy metals, chemical contaminations, and toxins from red tide algaes (Martello and Tjeerdema, 2001;Cheng et al, 2004;Machella et al, 2004;Yamasaki et al, 2004;Cho et al, 2006;Lannig et al, 2006;Lesser et al, 2006). Several epidemic diseases in marine animals are related to environmental stress and reduced immunity (Tanaka et al, 2002). Characterization of immune-related enzymes may not only lead to a better understanding of the immune defense mechanisms of marine animals, but will also provide insight into disease control and health management in the maricultural industry.…”

Section: Introductionmentioning

confidence: 99%

Identification and analysis of a Cu/Zn superoxide dismutase from Haliotis diversicolor supertexta with abalone juvenile detached syndrome

Sun

Cai

et al. 2010

Journal of Invertebrate Pathology

View full text Add to dashboard Cite

“…The LPO is readily decomposed to produce a variety of volatile compounds that are the main cause of undesirable odor in fish muscle (Tanaka et al 2013a(Tanaka et al , 2013b. In our laboratory, the stress in live fish (Tanaka et al 1999(Tanaka et al , 2002(Tanaka et al , 2006(Tanaka et al , 2012 and the quality of fish products (Tanaka et al 2013a(Tanaka et al , 2013b were evaluated by levels of LPO. However, LPO levels only reflect the current physiological condition in live fish.…”

mentioning

confidence: 99%

“…Therefore, estimating L-OH levels should provide another helpful index of oxidative stress in vivo. We developed a method to determine L-OH levels using a fluorescent reagent and high-performance liquid chromatography (HPLC) (Tanaka et al 1999(Tanaka et al , 2002. In these reports, L-OH levels were highest in the liver and then in red muscle followed by white muscle, based on activity in these tissues, and levels in cultured fish were significantly higher than those in wild fish, reflecting the degree of oxidative stress (Tanaka et al 1999).…”

mentioning

confidence: 99%

Effect of Short‐Term Holding without Feeding after Capture on Reduction in Oxidative Stress and Maintenance of Lipid and Amino Acid Contents in Decapterus maraudsi

Tanaka

Fukushima²,

Maeda³

et al. 2013

N American J Aquac

Self Cite

View full text Add to dashboard Cite

Changes in hydroxyl lipids (L‐OHs; a stress level indicator), body weight, triglycerides, fatty acids, and free amino acids (FAAs) were investigated in Decapterus maraudsi caught by purse seine from two different ocean regions near Japan to determine the effect of short‐term holding without feeding on the reduction in oxidative stress. In experiment 1, the L‐OHs in the dorsal muscle significantly decreased by 9 d, whereas those in the liver decreased significantly by the end of the 33‐d holding period. The body weight, lipid content, and triglyceride composition did not change significantly for 9 d and then decreased significantly by 33 d. Fatty acid composition of the dorsal muscle and liver did not change significantly within the holding period. The FAA content in the dorsal muscle did not change until 9 d and then decreased by 33 d. In experiment 2, the L‐OHs in the dorsal muscle had significantly increased at 33 d, whereas the levels in the liver of the fish after transport to port were twice as high as those in the fish that had just been caught, but then they decreased significantly by the end of the holding period. The change in body weight, lipid content, and triglyceride composition were the same as in experiment 1. The fatty acid compositions changed at 33 d, and the total FAA content did not change until 20 d and then decreased at 33 d. Both results suggest that to avoid stress and to obtain a higher quality fish product, fish captured by purse seine should be held without feeding for 8–9 d rather than immediately shipped after capture. The results of this study may be useful for improving the quality and commodity value of fish captured in commercial fisheries, such as by purse seine, and those held in aquaculture operations, such as in sea cages.

show abstract

Accumulation of hydroxy lipids in live fish infected with fish diseases

Cited by 17 publications

References 21 publications

An Assay Method for Determining the Total Lipid Content of Fish Meat Using a 2‐Thiobarbituric Acid Reaction

An Assay Method for Determining the Total Lipid Content of Fish Meat Using a 2‐Thiobarbituric Acid Reaction

Identification and analysis of a Cu/Zn superoxide dismutase from Haliotis diversicolor supertexta with abalone juvenile detached syndrome

Effect of Short‐Term Holding without Feeding after Capture on Reduction in Oxidative Stress and Maintenance of Lipid and Amino Acid Contents in Decapterus maraudsi

Contact Info

Product

Resources

About