Accumulation of intracellular trehalose and lactose in Lactobacillus plantarum WCFS1 during pulsed electric field treatment and subsequent freeze and spray drying

Vaessen, Evelien M.J.; Besten, H.M.W. den; Esveld, Erik; Schutyser, M.A.I.

doi:10.1016/j.lwt.2019.108478

Cited by 23 publications

(14 citation statements)

References 23 publications

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“…Therefore, relatively small sugar (i.e., disaccharides) such as trehalose might be ideal to stabilize the proteins under freeze-drying condition [ 25 ]. Trehalose has been used in protectant combination in many previous studies because of its excellent protective effect [ 4 , 5 , 8 , 26 , 27 ]. The performance of trehalose was also confirmed in this study.…”

Section: Discussionmentioning

confidence: 99%

Combinatorial Effects of Protective Agents on Survival Rate of the Yeast Starter, Saccharomyces cerevisiae 88-4, after Freeze-Drying

Chin

Lee

et al. 2021

Microorganisms

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A yeast starter is formulated for commercial practices, including storage and distribution. The cell viability of the yeast starter is one of the most important factors for manufacturing alcoholic beverages to ensure their properties during the fermentation and formulation processes. In this study, 64 potential protective agents were evaluated to enhance the survival rate of the brewing yeast Saccharomyces cerevisiae 88-4 after freeze-drying. In addition, the optimized combination of protective agents was assessed for long-term storage. Finally, response surface methodology was applied to investigate the optimal concentration of each protectant. Twenty of the 64 additives led to an increase in the survival rate of freeze-dried S. cerevisiae 88-4. Among the various combinations of protectants, four had a survival rate >95%. The combination of skim milk, maltose, and maltitol exhibited the best survival rate of 61% after 42 weeks in refrigerated storage, and the composition of protectants optimized by response surface methodology was 6.5–10% skim milk, 1.8–4.5% maltose, and 16.5–18.2% maltitol. These results demonstrated that the combination of multiple protectants could alleviate damage to yeasts during freeze-drying and could be applied to the manufacturing starters for fermented foods.

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Section: Discussionmentioning

confidence: 99%

Combinatorial Effects of Protective Agents on Survival Rate of the Yeast Starter, Saccharomyces cerevisiae 88-4, after Freeze-Drying

Chin

Lee

et al. 2021

Microorganisms

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“…Additionally, trehalose was also found intracellularly in L. plantarum WCFS1 after drying and reconstitution of the powder, when trehalose was used as the drying matrix. This finding suggests that the intracellular accumulation of trehalose during drying could be the reason why the PEF pre-treatment had no added value in this case [12].…”

Section: Introductionmentioning

confidence: 78%

“…In previous work we showed that pulsed electric field treatment can also be used to increase intracellular trehalose in bacteria, namely in Lactobacillus plantarum WCFS1 [11]. However, this did not lead to enhanced robustness of the PEF pre-treated cultures after drying and subsequent storage of the powders [12]. Remarkably, another recent study with a similar approach did find enhanced robustness of Lactobacillus bulgaricus upon spray drying with PEF pre-treatment to increase intracellular trehalose [13].…”

Section: Introductionmentioning

confidence: 86%

“…Lactobacillus plantarum WCFS1 was PEF treated in an IXL batch PEF system with custom made treatment chamber as described in previous research [12]. The PEF treatment consisted of 10 square wave pulses of 6.3 kV/cm with a pulse duration of 100 µs and pulse interval of 5 s. For every experiment, part of the bacterial culture was kept as a control (without PEF).…”

Section: Pulsed Electric Field Treatmentmentioning

confidence: 99%

“…Intracellular trehalose extraction and subsequent measurement with HPAEC analysis was performed according to the described in Vaessen et al (2019) [12]. Briefly, extracellular trehalose was washed away with three washing steps in phosphate buffered saline (PBS).…”

Section: Intracellular Trehalose Measurementsmentioning

confidence: 99%

See 2 more Smart Citations

Pulsed Electric Field Pre-treatment for Spray Drying of Probiotics

Besten¹,

Schutyser²

Proceedings of the 22nd International Drying Symposium on Drying Technology - IDS '22

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Pulsed electric field pre-treatment can be used for loading sugars into living cells, which may make cells more robust during subsequent spray drying. In this study we investigated the effect of the (change of) drying medium after PEF pre-treatment with trehalose for its impact on survival during subsequent spray drying for L. plantarum WCFS1. Our results showed that when trehalose was used as drying medium, no difference in survival with and without PEF pre-treatment was observed. However, when reconstituted skim milk and maltodextrin DE19 were used as drying medium an increased survival of the PEF-treated culture was observed compared to the control.

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Chemistry and Sources of Lactase Enzyme with an Emphasis on Microbial Biotransformation in Milk

Niamah

Al‐Sahlany

Verma

et al. 2023

Microbial Bioreactors for Industrial Molecules

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Accumulation of intracellular trehalose and lactose in Lactobacillus plantarum WCFS1 during pulsed electric field treatment and subsequent freeze and spray drying

Cited by 23 publications

References 23 publications

Combinatorial Effects of Protective Agents on Survival Rate of the Yeast Starter, Saccharomyces cerevisiae 88-4, after Freeze-Drying

Combinatorial Effects of Protective Agents on Survival Rate of the Yeast Starter, Saccharomyces cerevisiae 88-4, after Freeze-Drying

Pulsed Electric Field Pre-treatment for Spray Drying of Probiotics

Chemistry and Sources of Lactase Enzyme with an Emphasis on Microbial Biotransformation in Milk

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