Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy

Eguchi, Takanori; Kumagai, Koshi; Kobayashi, Hiroshi; Shigematsu, Hiroaki; Kitaura, Kazutaka; Suzuki, Shigeharu; Horikawa, Tsuyoshi; Hamada, Y.; Ogasawara, Kunio; Suzuki, Ryuji

doi:10.1016/j.cellimm.2013.07.010

“…NKT cells are thought to recognize endogenous glycolipids and secrete cytokines (such as IFN-γ) that help to amplify early immune responses [40]. In addition, we have identified the accumulation of NKT cells in the lesional skin of Ni allergy [16], [41]. These results imply that NKT cells participate in cross-reactivity of metal allergy.…”

Section: Discussionmentioning

confidence: 64%

“…How pathogenic T cells at the sites of allergic inflammation contribute to the development of metal allergy has not been explored because a suitable animal model has not been established. On the basis of previous reports [15]–[17], we generated a novel murine model of metal allergy, and have found the accumulation of the metal specific T cells in inflamed skin [16], [17].…”

Section: Introductionmentioning

confidence: 99%

Accumulation of Metal-Specific T Cells in Inflamed Skin in a Novel Murine Model of Chromium-Induced Allergic Contact Dermatitis

Shigematsu

¹

,

Kumagai

²

,

Kobayashi

³

et al. 2014

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Chromium (Cr) causes delayed-type hypersensitivity reactions possibly mediated by accumulating T cells into allergic inflamed skin, which are called irritants or allergic contact dermatitis. However, accumulating T cells during development of metal allergy are poorly characterized because a suitable animal model is not available. This study aimed to elucidate the skewing of T-cell receptor (TCR) repertoire and cytokine profiles in accumulated T cells in inflamed skin during elucidation of Cr allergy. A novel model of Cr allergy was induced by two sensitizations of Cr plus lipopolysaccharide solution into mouse groin followed by single Cr challenge into the footpad. TCR repertoires and nucleotide sequences of complementary determining region 3 were assessed in accumulated T cells from inflamed skin. Cytokine expression profiles and T-cell phenotypes were determined by qPCR. CD3+CD4+ T cells accumulated in allergic footpads and produced increased T helper 1 (Th1) type cytokines, Fas, and Fas ligand in the footpads after challenge, suggesting CD4+ Th1 cells locally expanded in response to Cr. Accumulated T cells included natural killer (NK) T cells and Cr-specific T cells with VA11-1/VB14-1 usage, suggesting metal-specific T cells driven by invariant NKT cells might contribute to the pathogenesis of Cr allergy.

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“…NKT cells are thought to recognize endogenous glycolipids and secrete cytokines (such as IFN-c) that help to amplify early immune responses [40]. In addition, we have identified the accumulation of NKT cells in the lesional skin of Ni allergy [16,41]. These results imply that NKT cells participate in cross-reactivity of metal allergy.…”

Section: Discussionmentioning

confidence: 66%

“…How pathogenic T cells at the sites of allergic inflammation contribute to the development of metal allergy has not been explored because a suitable animal model has not been established. On the basis of previous reports [15][16][17], we generated a novel murine model of metal allergy, and have found the accumulation of the metal specific T cells in inflamed skin [16,17].…”

Section: Introductionmentioning

confidence: 99%

Accumulation of metal-specific T cells in inflamed skin in a novel murine model of Chromium-induced allergic contact dermatitis

Shigematsu¹,

Kumagai²,

Kobayashi³

et al. 2014

Journal of Oral and Maxillofacial Surgery

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Chromium (Cr) causes delayed-type hypersensitivity reactions possibly mediated by accumulating T cells into allergic inflamed skin, which are called irritants or allergic contact dermatitis. However, accumulating T cells during development of metal allergy are poorly characterized because a suitable animal model is not available. This study aimed to elucidate the skewing of T-cell receptor (TCR) repertoire and cytokine profiles in accumulated T cells in inflamed skin during elucidation of Cr allergy. A novel model of Cr allergy was induced by two sensitizations of Cr plus lipopolysaccharide solution into mouse groin followed by single Cr challenge into the footpad. TCR repertoires and nucleotide sequences of complementary determining region 3 were assessed in accumulated T cells from inflamed skin. Cytokine expression profiles and T-cell phenotypes were determined by qPCR. CD3+CD4+ T cells accumulated in allergic footpads and produced increased T helper 1 (Th1) type cytokines, Fas, and Fas ligand in the footpads after challenge, suggesting CD4+ Th1 cells locally expanded in response to Cr. Accumulated T cells included natural killer (NK) T cells and Cr-specific T cells with VA11-1/VB14-1 usage, suggesting metal-specific T cells driven by invariant NKT cells might contribute to the pathogenesis of Cr allergy.

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“…NKT cells were activated to produce IL-4 and IL-13 in response to CD1d-presented haptens to suppress CHS. In contrast, other studies suggest that NKT cells exacerbate hypersensitivity responses [81,82] and have shown that murine NKT cells accumulate in the skin following sensitization to nickel [83], whereas large numbers of NKT cells can also infiltrate human skin during contact dermatitis [10,84]. In accordance with enrichment of NKT cells in the skin, studies of the peripheral blood in human atopic dermatitis demonstrate a reduction in NKT cells [85].…”

Section: Nkt Cells In Hypersensitivity Responses In the Skinmentioning

confidence: 97%

Immunosuppressive roles of natural killer T (NKT) cells in the skin

McKee

¹

,

Mattarollo

²

,

Leggatt

³

2014

Journal of Leukocyte Biology

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The skin is a complex immunological niche providing immunity to invading pathogens while simultaneously maintaining tolerance to innocuous environmental antigens. Consistent with this complex response, the skin is resident to both immunosuppressive and effector cell populations whose activities are tightly regulated. While NKT cells can activate immune responses in the skin, this review will highlight studies on UV-induced photodamage, models of NMSCs, transplantation and allergic inflammation where NKT cells appear to have an immunosuppressive role in the skin.

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Accumulation of invariant NKT cells into inflamed skin in a novel murine model of nickel allergy

Cited by 27 publications

References 35 publications

Accumulation of Metal-Specific T Cells in Inflamed Skin in a Novel Murine Model of Chromium-Induced Allergic Contact Dermatitis

Accumulation of Metal-Specific T Cells in Inflamed Skin in a Novel Murine Model of Chromium-Induced Allergic Contact Dermatitis

Accumulation of metal-specific T cells in inflamed skin in a novel murine model of Chromium-induced allergic contact dermatitis

Immunosuppressive roles of natural killer T (NKT) cells in the skin

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