Accumulation of l-DOPA in various organs of faba bean and influence of drought, nitrogen stress, and processing methods on l-DOPA yield

Etemadi, Fatemeh; Hashemi, Masoud; Randhir, Reena; Zandvakili, Omid Reza; Ebadi, Abbas

doi:10.1016/j.cj.2017.12.001

Cited by 58 publications

(67 citation statements)

References 32 publications

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“…dry matter produced at the time of harvest was lower than for other experiments conducted at the same location (e.g., Etemadi et al, 2018a). We found no statistical significance for the interaction of year by dry matter and L-DOPA concentration; therefore, an average of the 2 yr is presented.…”

Section: Standard Solutioncontrasting

confidence: 72%

“…Currently published studies that have evaluated L-DOPA content of faba bean were mainly focused on fresh and dry seeds (Longo et al, 1974;Shetty et al, 2001;Randhir and Shetty, 2004). Recently, we reported that leaves were the richest L-DOPA organ of faba bean, followed by flowers, young pods, mature seeds, roots, and stems, respectively (Etemadi et al, 2018a). Recently, we reported that leaves were the richest L-DOPA organ of faba bean, followed by flowers, young pods, mature seeds, roots, and stems, respectively (Etemadi et al, 2018a).…”

Section: Discussionmentioning

confidence: 99%

“…The procedure for the extraction of L-DOPA has been described fully in our recent publication (Etemadi et al, 2018a). Two hundred milligrams of dried sample was immersed in 95% ethanol and kept in the freezer for 72 h. Samples were then homogenized using a Bio Homogenizer (BioSpec Products).…”

Section: Extraction Methodsmentioning

confidence: 99%

“…in terms of L-DOPA content (Soares et al 2014). Laurent et al, 2002;Etemadi et al, 2017a), environmental conditions (Goyoaga et al, 2008;Multari et al, 2015), plant growth stage (Geng, 2012), and organs (Etemadi et al, 2018a). Laurent et al, 2002;Etemadi et al, 2017a), environmental conditions (Goyoaga et al, 2008;Multari et al, 2015), plant growth stage (Geng, 2012), and organs (Etemadi et al, 2018a).…”

mentioning

confidence: 99%

“…However many earlier reports have been focused on L-DOPA content in seeds, whether as fresh or dried, and less attention has been given to the amount of L-DOPA in other parts of the plant. However, L-DOPA yield is a product of concentration and plant biomass (Etemadi et al, 2018a), and thus the highest L-DOPA yield can be obtained at later stages of growth. However, L-DOPA yield is a product of concentration and plant biomass (Etemadi et al, 2018a), and thus the highest L-DOPA yield can be obtained at later stages of growth.…”

mentioning

confidence: 99%

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Yield and Accumulation Trend of Biomass and L‐DOPA in Different Parts of Eight Faba Bean Cultivars

et al. 2018

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Faba bean (Vicia faba L.) is a rich source of 3,4‐dihydroxyphenyl‐L‐alanine (L‐DOPA) and thus can be used as an alternative to synthetic compounds for curing Parkinson's disease. Accumulation of biomass and L‐DOPA in different organs of eight cultivars of faba bean was investigated in a field‐grown study in 2015 and 2016 at the Research and Education Center, University of Massachusetts, Amherst. Plants were harvested at five stages of growth and partitioned into various organs including roots, leaves, stems, pod walls, and fresh seeds. ‘Aquadulce’ and ‘Delle Cascine’ outyielded other cultivars in both years. Averaged over 2 yr, Aquadulce and Delle Cascine produced 16,152 and 16,270 kg fresh pods ha−1. Thus, the highest L‐DOPA seed yield was obtained from these two cultivars. Accumulation of L‐DOPA during faba bean growing period exhibited a quadratic trend and reached its peak values at 65 to 75 d after transplanting, whereas accumulation of dry matter exhibited a linear trend. The results revealed that distribution of dry matter among various organs of faba bean was significantly different among the cultivars. The L‐DOPA yield (concentration × dry matter) varied dramatically among the cultivars where Aquadulce and ‘Sweet Lorane’ yielded 55.2 and 6.4 kg L‐DOPA ha−1, respectively. The superiority of Aquadulce in regard to the L‐DOPA yield was primarily due to its photosynthesis capacity, which produced 6871 kg dry matter ha−1, rather than its L‐DOPA concentration. We concluded that if the goal of producing faba bean is dual purpose, the leaves can be used as the richest L‐DOPA organ, whereas the green pods can be harvested as a cash crop.

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Section: Standard Solutioncontrasting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Extraction Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Yield and Accumulation Trend of Biomass and L‐DOPA in Different Parts of Eight Faba Bean Cultivars

et al. 2018

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A virus‐induced gene‐silencing system for functional genetics in a betalainic species, Amaranthus tricolor (Amaranthaceae)

et al. 2019

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Premise of the Study Research in Amaranthaceae could be accelerated by developing methods for targeted gene silencing. Most amaranths, including Amaranthus tricolor, produce betalains. However, the physiological and ecological roles of these pigments are uncertain. We sought to establish a virus‐induced gene‐silencing (VIGS) method for amaranths, using silencing of betalain pigments as a proof‐of‐principle. Methods We targeted AtriCYP76AD1, a putative cytochrome P450 component of the betalain biosynthetic pathway, using VIGS, and compared two different methods of introducing the VIGS construct into plants. We measured transcript abundance and concentrations of betalains and their l‐DOPA precursor in VIGS‐treated plants, and compared these to controls. Results We observed that when AtriCYP76AD1 was targeted by VIGS in normally red plants, AtriCYP76AD1 and the related genes AtriCYP76AD6 and AtriCYP76AD5 had diminished transcript abundance. Furthermore, newly emergent petioles and leaves of VIGS‐treated plants appeared green, betacyanin accumulation was strongly reduced, and l‐DOPA accumulation was increased. No betaxanthin could be detected in this variety of A. tricolor, either before or after VIGS treatment. Discussion These results help to establish the genetic basis of betalain synthesis in amaranths. Furthermore, this is the first report of VIGS in amaranths and demonstrates the potential of this technique for basic and applied research in these species.

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A validated LC–MS/MS method for quantitative determination of L‐dopa in Fagioli di Sarconi beans (Phaseolus vulgaris L.)

et al. 2023

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An analytical method based on ultrasound assisted extraction (UAE) and liquid chromatography coupled to electrospray tandem mass spectrometry (LC–ESI/MS/MS) was validated and applied for determining L‐dopa in four ecotypes of Fagioli di Sarconi beans (Phaseolus vulgaris L.), marked with the European label PGI (Protected Geographical Indication). The selectivity of the proposed method was ensured by the specific fragmentation of the analyte. Simple isocratic chromatographic conditions and mass spectrometric detection in multiple reaction monitoring (MRM) acquisition mode were used for sensitive quantification. The LC–ESI/MS/MS method was validated within a linear range of 0.001–5.000 μg/mL. Values of 0.4 and 1.1 ng/mL were obtained for the limits of detection and quantification, respectively. The repeatability, inter‐day precision, and recovery values ranges were 0.6%–4.5%, 5.4%–9.9%, and 83%–93%, respectively. Fresh and dried beans, as well as pods, cultivated exclusively with organic methods avoiding any synthetic fertilizers and pesticides were analyzed showing an L‐dopa content ranging from 0.020 ± 0.005 to 2.34 ± 0.05 μg/g dry weight.

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Accumulation of l-DOPA in various organs of faba bean and influence of drought, nitrogen stress, and processing methods on l-DOPA yield

Cited by 58 publications

References 32 publications

Yield and Accumulation Trend of Biomass and L‐DOPA in Different Parts of Eight Faba Bean Cultivars

Yield and Accumulation Trend of Biomass and L‐DOPA in Different Parts of Eight Faba Bean Cultivars

A virus‐induced gene‐silencing system for functional genetics in a betalainic species, Amaranthus tricolor (Amaranthaceae)

A validated LC–MS/MS method for quantitative determination of L‐dopa in Fagioli di Sarconi beans (Phaseolus vulgaris L.)

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