Accumulation of Lipids and Triglycerides in Isochrysis galbana Under Nutrient Stress

El-Din, Soad M. Mohy

doi:10.1007/s12010-019-02997-0

Cited by 10 publications

(9 citation statements)

References 32 publications

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“…The small proportion of PL in I. galbana can be explained by the phase at which biomass was harvested, presenting marine microalgae species during the stationary phase an increase in TAG with a corresponding decrease in PL [ 30 ]. The limitation of nutrients for microalgae can be considered an important technique for FA and TAG production [ 34 ]. Microalgae growth can slow down under stress conditions, wherein the microalgal cells do not synthesize new membrane substances and FA are channeled to TAG synthesis as a mechanism of protection [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

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Yogurt Enriched with Isochrysis galbana: An Innovative Functional Food

Matos

Afonso

Cardoso

et al. 2021

Foods

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Microalgae are a valuable and innovative emerging source of natural nutrients and bioactive compounds that can be used as functional ingredients in order to increase the nutritional value of foods to improve human health and to prevent disease. The marine microalga Isochrysis galbana has great potential for the food industry as a functional ingredient, given its richness in ω3 long chain-polyunsaturated fatty acids (LC-PUFAs), with high contents of oleic, linoleic, alpha-linolenic acid (ALA), stearidonic, and docosahexaenoic (DHA) acids. This study focuses on the formulation of a functional food by the incorporation of 2% (w/w) of I. galbana freeze-dried biomass and 2% (w/w) of I. galbana ethyl acetate lipidic extract in solid natural yogurts preparation. In the functional yogurt enriched with microalgal biomass, the ω3 LC-PUFA’s content increased (to 60 mg/100 g w/w), specifically the DHA content (9.6 mg/100 g ww), and the ω3/ω6 ratio (augmented to 0.8). The in vitro digestion study showed a poor bioaccessibility of essential ω3 LC-PUFAs, wherein linoleic acid (18:2 ω6) presented a bioaccessibility inferior to 10% and no DHA or eicosapentaenoic acid (EPA) was detected in the bioaccessible fraction of the functional yogurts, thus indicating a low accessibility of lipids during digestion. Notwithstanding, when compared to the original yogurt, an added value novel functional yogurt with DHA and a higher ω3 LC-PUFAs content was obtained. The functional yogurt enriched with I. galbana can be considered important from a nutritional point of view and a suitable source of essential FAs in the human diet. However, this needs further confirmation, entailing additional investigation into bioavailability through in vivo assays.

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Section: Resultsmentioning

confidence: 99%

“…The limitation of nutrients for microalgae can be considered an important technique for FA and TAG production [ 34 ]. Microalgae growth can slow down under stress conditions, wherein the microalgal cells do not synthesize new membrane substances and FA are channeled to TAG synthesis as a mechanism of protection [ 34 ].…”

Section: Resultsmentioning

confidence: 99%

Yogurt Enriched with Isochrysis galbana: An Innovative Functional Food

Matos

Afonso

Cardoso

et al. 2021

Foods

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“…In our previous study, marine macroalga Rhizoclonium africanum showed maximum growth and lipid production at limited nitrogen [13]. Most of the works on biomass and lipid accumulation had been done using unicellular microalga [6,[28][29][30][31][32], while very few reports are available on filamentous macroalga [12,14,33]. In the present study different concentrations of four nutrients like nitrogen, phosphorus, EDTA and NaCl were introduced in the culture medium to study the growth pattern in terms of biomass yield and lipid accumulation in marine macroalga C. aerea.…”

Section: Discussionmentioning

confidence: 99%

Effects of nitrogen, phosphorus, EDTA and sodium chloride on biomass and lipid accumulation of Chaetomorpha aerea

Satpati¹,

Pal²

2020

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The increase of total lipid and fatty acids production was studied under different nutrient stress conditions using the macroalga, Chaetomorpha aerea. The effects of nitrogen, phosphorus, ethylene diamine tetra-acetate and sodium chloride on the growth and lipid accumulation were systematically investigated in laboratory conditions. The biomass was harvested at different stages of cultivation and assessed. The maximum changes of growth and lipid accumulation were observed in the exponential phase at different cultural conditions. The two-fold increase of total lipid was found in the order of 28.27±0.04 % (at 0.1 g/L nitrogen) > 27.30±0.37 % (at 0.5 g/L of phosphorus) > 25.86±0.77 % (at 0.05 g/L of EDTA)> 24.37±0.04 % (at 0.05 g/L NaCl) on 8th day of cultivation. The fatty acids were identified and quantified by gas chromatography mass spectrometry (GC-MS). The alga produces significantly high amount of monounsaturated fatty acid (MUFA) and saturated fatty acids (SFA) than the polyunsaturated fatty acids (PUFA) in different cultural conditions. The elevated levels of C16:1, C18:1 and C20:1 was identified under nitrate, phosphate and salt stress conditions, which are more suitable for biodiesel production.

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“…Among these, nitrogen depletion and length of photoperiods have most significant influence on both biomass and lipid productivity (LP). Studies showed that the biosynthetic pathways for creation and storage of lipids (hydrocarbons and triacylglycerols) of microalgae may change according to the availability of nitrogen and length of photoperiods 22,23 …”

Section: Introductionmentioning

confidence: 99%

“…Studies showed that the biosynthetic pathways for creation and storage of lipids (hydrocarbons and triacylglycerols) of microalgae may change according to the availability of nitrogen and length of photoperiods. 22,23 Illman et al 24 reported the impacts of nitrogen dose on lipid amount of five different Chlorella strains. A decrease in nitrogen dose in the culture media increased the lipid concentration of all five studied Chlorella strains, although the biomass productivity increased.…”

Section: Introductionmentioning

confidence: 99%

Optimization of microalgal biomass and lipid productivities for bioenergy production using central composite design with desirability function

Hossain

Sultana

Hossain

et al. 2021

Intl J of Energy Research

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This study investigates the influences of light-dark cycles and NaNO 3 dose on Chlorella kessleri microalgal-specific growth rate (SGR), biomass productivity (P), and intracellular lipid productivity (LP) by using central composite design with desirability function. A single-objective optimization showed that the maximum SGR, P, and LP can be achieved as 0.487 day −1 , 58.718 g/L day −1 , and 14.252 mg/L day −1 , respectively. The corresponding optimized light-dark cycle and NaNO 3 loading were determined as 11.6/8.4 (h/h) and 15.54 g/L, 12/12 (h/h) and 16.23 g/L, and 12/12 (h/h) and 6.63 g/L, respectively. The multiresponse optimization showed that the highest SGR of 0.359 day −1 , biomass productivity of 50.663 g/L day −1 and LP of 14.114 mg/L day −1 can be accomplished at culture environments of 12/12 (h/h) light-dark cycles and 10.38 g/L NaNO 3 . These results suggest that the central composite design with desirability function-based technique is useful for design and operation of photobioreactors to produce lipid from microalgae. Analysis of variance was employed to explore the significance of the factors affecting microalgal growth as well as biomass and lipid productivities. The predicted optimized culture conditions were experimentally confirmed (error < 6%) indicating the process is robust and reliable.

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Accumulation of Lipids and Triglycerides in Isochrysis galbana Under Nutrient Stress

Cited by 10 publications

References 32 publications

Yogurt Enriched with Isochrysis galbana: An Innovative Functional Food

Yogurt Enriched with Isochrysis galbana: An Innovative Functional Food

Effects of nitrogen, phosphorus, EDTA and sodium chloride on biomass and lipid accumulation of Chaetomorpha aerea

Optimization of microalgal biomass and lipid productivities for bioenergy production using central composite design with desirability function

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