Accumulation of madecassoside - a major component of centelloside - in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid

Lộc, Nguyễn Hoàng; Giang, Nguyen Thanh; Huy, Nguyễn Đức; Lan, Truong Thi Phuong

doi:10.18054/pb.v119i1.4386

Cited by 10 publications

(4 citation statements)

References 17 publications

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“…In particular, inoculum size had a positive effect on biomass and metabolite production up to an optimum concentration 28 . In this study, the optimal inoculum size for biomass accumulation and gypenoside production was 3 g of callus per 50 ml culture medium (6% w/v ), which is similar to that reported by Loc et al for the production of madecassoside in Centella asiatica (L.) suspension cell culture 29 . Nhan et al also found that an inoculum size of 6% ( w/v ) is optimal for eurycomanone biosynthesis in long jack ( Eurycoma longifolia ) suspension cells culture 24 .…”

Section: Discussionsupporting

confidence: 85%

Gypenosides Production and Spermatogenesis Recovery Potentials of Extracts from Cell Suspension Cultures of Gynostemma pentaphyllum

Nguyen-Thanh,

Dang-Ngoc,

Tran-Quoc

et al. 2023

AJMB

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Background: Gynostemma pentaphyllum (GP), also called Giao-co-lam, is a traditional Vietnamese herb, also known as the "Herb of Immortality", that grows throughout Asian countries and is used for the treatment of hematuria, edema in the pharynx and neck, tumors, and trauma. Methods: In this study, we investigated the effects of culture conditions on cell growth and total gypenoside accumulation in the GP suspension cells. Cells were cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L KIN and 0.5 mg/L IBA, and different inoculum sizes (2-4 g) for 10-24 days. Results: The results showed that the optimum inoculum size and shaking speed were 3 g of callus and 120 rpm, respectively. The highest cell biomass reached was 5.833 g of fresh weight, corresponding to 0.136 g of dry weight after 20 days of culture. The total gypenoside and Rb1 accumulation was the highest after 18 days of culture, with 46.498 mg/g and 0.038 mg/g dry weight, respectively. In addition, the crude extract from GP cell suspension cultures remarkably improved pathological changes in mouse testicular tissue after scrotal heat exposure. Blood testosterone levels were significantly increased in heat-exposed mice treated with the GP cell suspension culture extract. Conclusion: Taken together, these results suggest that GP bio-mass production by cell suspension cultures leads to the accumulation of gypenosides in large amounts, and provides the potential for the recovery of spermatogenesis following heat stress.

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Section: Discussionsupporting

confidence: 85%

Gypenosides Production and Spermatogenesis Recovery Potentials of Extracts from Cell Suspension Cultures of Gynostemma pentaphyllum

Nguyen-Thanh,

Dang-Ngoc,

Tran-Quoc

et al. 2023

AJMB

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“…We investigated the differential expression of genes involved in triterpenoid pathway as well as of miRNAs in SA augmented, in vitro grown cultures of C. asiatica. SA is a potent elicitor for increasing triterpenoid content in C. asiatica as many workers have experimentally proved it multiple times [87][88][89][90]. This could provide an indication whether effects of SA on centelloside biosynthesis could be mediated by changes in miRNAs.…”

Section: Discussionmentioning

confidence: 98%

Unraveling MicroRNA Mediated Gene Regulation in Centella asiatica (L.) Urb. by high-Throughput Sequencing Based Small-RNA Profiling

Ranjith

Satheesan

Sabu

2023

Tropical Plant Biol.

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Centella asiatica is a widely spread herb mostly found in the tropics having extensive medicinal values. Here, we report for the rst time, transcriptome-wide characterization of miRNA pro le from the leaves of C. asiatica using high-throughput Illumina sequencing. We identi ed 227 conserved and 109 putative novel miRNAs. Computational screening revealed potential mRNA targets for both the conserved and novel miRNAs encoding diverse transcription factors and enzymes involved in plant development, disease resistance, metabolic and signaling pathways. Gene ontology annotation and KEGG analysis revealed the miRNA targets to be involved in a wide range of metabolomic and regulatory pathways. The differential expression of the miRNA encoding genes in diverse tissues was determined by real-time PCR analysis. We also found that gene expression levels of miR156, 159 and 1171 was reduced in salicylic acid treated axenic shoot cultures of C. asiatica compared to its control. Furthermore, RLM-RACE experiments mapped miRNA-mediated cleavage at two of the mRNA targets. The present study represents the large-scale identi cation of microRNAs from C. asiatica and contributes to the base for the up-coming studies on miRNA-mediated gene regulation of plant secondary metabolite pathways in particular.

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“…Elicitation was performed with 1 µM of coronatine (COR) and 150 µM of salicylic acid (SA) (Sigma Aldrich, St. Louis, MO, USA), which were previously filter-sterilized and added to the cell suspensions. The SA concentration was selected based on Loc et al [ 56 ] and Alcalde et al [ 27 ] studies.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Interplay between Metabolic Pathways and Taxane Production in Elicited Taxus baccata Cell Suspensions

Perez-Matas

García-Pérez

Miras-Moreno

et al. 2023

Plants

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Taxus cell cultures are a reliable biotechnological source of the anticancer drug paclitaxel. However, the interplay between taxane production and other metabolic pathways during elicitation remains poorly understood. In this study, we combined untargeted metabolomics and elicited Taxus baccata cell cultures to investigate variations in taxane-associated metabolism under the influence of 1 µM coronatine (COR) and 150 µM salicylic acid (SA). Our results demonstrated pleiotropic effects induced by both COR and SA elicitors, leading to differential changes in cell growth, taxane content, and secondary metabolism. Metabolite annotation revealed significant effects on N-containing compounds, phenylpropanoids, and terpenoids. Multivariate analysis showed that the metabolomic profiles of control and COR-treated samples are closer to each other than to SA-elicited samples at different time points (8, 16, and 24 days). The highest level of paclitaxel content was detected on day 8 under SA elicitation, exhibiting a negative correlation with the biomarkers kauralexin A2 and taxusin. Our study provides valuable insights into the intricate metabolic changes associated with paclitaxel production, aiding its potential optimization through untargeted metabolomics and an evaluation of COR/SA elicitor effects.

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Accumulation of madecassoside - a major component of centelloside - in centella (Centella asiatica (L.) Urban) cells elicited by salicylic acid

Abstract: Background and Purpose: Use of elicitors is one of the most important methods to increase the production of secondary metabolites in plants. Salicylic

Cited by 10 publications

References 17 publications

Gypenosides Production and Spermatogenesis Recovery Potentials of Extracts from Cell Suspension Cultures of Gynostemma pentaphyllum

Gypenosides Production and Spermatogenesis Recovery Potentials of Extracts from Cell Suspension Cultures of Gynostemma pentaphyllum

Unraveling MicroRNA Mediated Gene Regulation in Centella asiatica (L.) Urb. by high-Throughput Sequencing Based Small-RNA Profiling

Exploring the Interplay between Metabolic Pathways and Taxane Production in Elicited Taxus baccata Cell Suspensions

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