Accumulation of mineral oil saturated hydrocarbons (MOSH) in female Fischer 344 rats: Comparison with human data and consequences for risk assessment

Mineral oil in food, cosmetic products, and in products regulated by other legislations

“…food samples) are present in lower concentrations, it is not necessary to remove them from the sample in order to be able to achieve a proper quantitation of MOSH. The removal step was not applied for the analysis of MOSH in biological tissues and breast milk (Noti et al 2003;Concin et al 2008; Barp et al 2014;; Barp et al 2017aBarp et al , 2017b.…”

“…Further insights into the intestinal absorption of mineral oils comes from the measurement of MOSH in tissue. In a recent study, Barp et al (2017a) exposed female F-344 rats to a diet containing 0.004% of a broad MOSH mixture with a carbon number range from C 14 to C 50 . The test item was dissolved in olive oil before incorporating it into the diet.…”

“…Information on the metabolism of complex MOSH mixtures can be derived from GC Â GC analysis of hydrocarbons that are retained in tissue. In a recent study, Barp et al (2017a) exposed female F-344 rats to a diet containing a broad MOSH mixture, ranging from about C 14 to C 50 and being largely devoid of n-alkanes above C 21 , over a period of 90 days, followed by a 30-day post-exposure period. Hydrocarbons retained in liver ranged from C 16 to C 40 .…”

Outcome of the public consultation on the terms of reference for the development of a guidance document on “Harmonisation of risk assessment methodologies for human health and ecological risk assessment of combined exposure to multiple chemicals”

“…For example, it is well known that the F344 rat reacts adversely to n-alkanes, which is indicative that the F344 rat model is not adequate for risk assessment purposes. This is supported by the fact that n-alkanes are not selectively retained in human livers, but nalkanes are on the other hand ubiquitously retained in the livers of F344 rats (Fleming et al, 1998;Barp et al, 2014Barp et al, , 2016. Our recommendation is that the reproducibility of (adverse) effects in various strains and species and humans should be objectively weighed to clearly distinguish "the most sensitive" model from "inadequate models" for hazard and risk assessment.…”

Kontaminanten aus Lebensmittelverpackungen

“…Van Bossuyt et al [17] schätzen, dass mehr als 6000 Einzelverbindungen in bedruckten Papieren und Kartonagen vorkommen, wobei die Mehrheit (77 %) nicht toxikologisch bewertet ist und viele davon oral aufgenommen werden können. Als weiteres Kontaminantenproblem ist die Exposition des Verbrauchers gegenüber Gemischen von (teilweise bewerteten) Einzelsubstanzen wie Phthalaten [18] oder Perfluorverbindungen [19] sowie komplexen Gemischen aus nicht völlig identifizierten Komponenten wie Mineralöl-Kohlenwasserstoffen [20] oder dem "forest of peaks" aus Beschichtungen [21] zu nennen. Einen wesentlichen Anteil an den migrierenden Stoffen können sogenannte nicht beabsichtigt zugesetzte Substanzen (non-intentionally added substances, NIASs) wie Verunreinigungen, Reaktionsprodukte oder Oligomere haben, die bei der Substanzbewertung durch die Europäische Lebensmittelsicherheitsbehörde (EFSA) und die nationalen Behörden (z.…”