Accumulation of miRNA and mRNA Targets in Potato Leaves Displaying Temperature-Dependent Responses to Potato Virus Y

Abstract

We demonstrate temporal-, spatial-and temperature-dependent expression of five host microRNAs and target mRNAs in potato leaves after potato virus Y (PVY) inoculation. A novel gene, Ny-DG, conferring resistance to PVY was mapped on chromosome IX in the diploid potato clone DG 81-68. At 20°C, the PVY NTN strain was localized in the inoculated leaves without symptoms. At 28°C, necrotic lesions were observed in both the inoculated leaves and the non-inoculated upper leaves and the virus spread systemically, which indicates overcoming the resistance. At 0.5 h post-inoculation and 3-day postinoculation (dpi), PVY led to significant changes in the levels of the tested miRNAs and their targets only in a small number of cases. At 6 dpi, in the inoculated leaves at 28°C, the increased expression of stu-miR162, stu-miR168a and stu-miR482 (PotatoMir1005353123_x15854) promoted the downregulation of their targets DCL1, AGO1-2 and Cc-nbs-lrr, respectively. The expression of stu-miR482 (PotatoMir1005658171_x16170) and stu-miR172e remained unchanged, whereas their targets Gpa2 and TOE3 were downregulated. However, in the inoculated leaves at 20°C, all five tested miRNAs and their targets showed parallel downregulation. In the noninoculated upper leaves, changes in gene expression levels, namely downregulation, were only detected for the tested five miRNAs at 28°C. We conclude that the expression patterns of the tested miRNAs and targets were altered differently not only at different time points post-inoculation but also in the inoculated and upper leaves, and their accumulation levels were related to the type of reaction, which in turn was dependent on temperature.

Reaction of three potato cultivars to potato virus S and alteration of miRNAs and mRNA targets in the resistant genotypes

“…Elevated expression of AGO1-2, DCL1 and stu-miR168a in the PVS-resistant cvs Tonacja and Tajfun but not in the susceptible cv. Bryza demonstrated in our present study, as well as their altered expression in PVY-resistant genotypes in previous studies (Szajko et al, 2019;, indicates their involvement in potato resistance to PVS and PVY. (h) Gpa2 (PGSC0003DMT400050510), disease resistance protein Gpa2; (i) stu-miR482_15854 (PotatoMir1005353123_x15854); (j) CC-NBS-LRR (PGSC0003DMT400019599), CC-NBS-LRR resistance protein, JHL06P13.14 protein.…”

“…Studies of host miRNAs in plant–virus interaction have been documented in 29 plant species and 12 DNA and 38 RNA virus species (Yin, 2021). In potato, changes in host miRNA expression in response to viral infection have been reported for two virus species, potato virus A (PVA) and potato virus Y (PVY) (Križnik et al, 2017; Li et al, 2017; Szajko et al, 2019; Yin et al, 2017). A total of 201 differentially expressed miRNAs were identified in the PVA‐hypersensitive potato A6 ( Solanum demissum × Aquila) following PVA infection (Li et al, 2017).…”

“…virus A (PVA) and potato virus Y (PVY) (Križnik et al, 2017;Li et al, 2017;Szajko et al, 2019;. A total of 201 differentially expressed miRNAs were identified in the PVA-hypersensitive potato A6 (Solanum demissum × Aquila) following PVA infection (Li et al, 2017).…”

Plant Molecular Responses to Potato Virus Y: A Continuum of Outcomes from Sensitivity and Tolerance to Resistance

“…The Ry hou gene was found in S. hougasii [78] but it has not been used for breeding. Additionally, the Ny-DG gene present in the diploid potato clone DG-68 features ER-like response [79]. Recently, a dominant resistant gene Ry(o) phu was identified in S. tuberosum ssp.…”

LuluDB—The Database Created Based on Small RNA, Transcriptome, and Degradome Sequencing Shows the Wide Landscape of Non-coding and Coding RNA in Yellow Lupine (Lupinus luteus L.) Flowers and Pods

“…Literature data contain evidence that miR162 regulates the DCL1 expression in other plants (Xie et al, 2003;Liu et al, 2014a;Szajko et al, 2019), whereas in yellow lupine, we have identified a new regulator of DCL2 (Glazinska et al, 2019), a novel miRNA named "Ll-miRn30, " deposited in the database under ID486.…”