Accumulation of peonidin 3-glucoside enhanced by osmotic stress in grape (Vitis vinifera L.) cell suspension

Bao, Chi; Cormier, François

doi:10.1007/bf00044265

Cited by 68 publications

(27 citation statements)

References 21 publications

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“…When comparing 3% to 6% sugar in EM medium, 6% sugar resulted in the highest anthocyanin production (Figure 3). The same phenomena have been reported in Vitis vinifera by Do and Cormier (1991) and Pasqua et al (2005). It is considered that glycosylation of cyanidin might be promoted if cells took up much more sugar from the sugar rich medium because the level of UF3GT gene expression obviously increased with high sugar EM medium (Figure 4).…”

Section: Effects Of Medium Components and Light Irradiation On Anthocsupporting

confidence: 79%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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A suspension cultured cell line was established from the cultivar of Rosa hybrida 'Charleston' as a study model to understand the response of the anthocyanin biosynthesis pathway to environmental cues. The major identified anthocyanin in cell cultures was cyanidin 3-glucoside (chrysanthemin). The anthocyanin yield was enhanced by culturing cells in the EM medium with added sucrose at high concentration under additional UV-B radiation to white light. Three cDNA fragments were cloned with degenerate primers by RT-PCR and the obtained sequences shared high homology with putative key enzymes (DFR, ANS, and UF3GT) of other species. The expression levels of these three genes were promoted under optimum conditions for anthocyanin accumulation. These results suggest that expression levels of these genes were closely correlated with a temporal buildup of anthocyanins in response to environmental factors.Key words: Anthocyanin, chrysanthemin, cyanin, Rosa hybrida, suspension cell culture.Plant Biotechnology 23, 379-385 (2006) Original PaperThis article can be found at http://www.jspcmb.jp/ pelargonidin, cyanidin and peonidin type anthocyanins (Biolley et al. 1994). Recently, tissue culturing has become an important biotechnological technique to produce various secondary metabolites including anthocyanins on a mass scale for industrial and academic interest (Hirner et al. 2001;Gantet and Memelink 2002). Despite the fact that some plant species have been shown to produce anthocyanins in cell cultures, little information of the cell culture techniques that are capable of producing in vitro anthocyanins has been reported in rose.The biosynthesis of anthocyanins in plant cell culture is stimulated by not only genetic factors but also environmental factors such as light, temperature, growth regulators and nutrition (Sato et al. 1996;Zhang et al. 1997;Zhang et al. 2002). The identity of the environmental stimuli that lead to the accumulation of anthocyanins in rose is of considerable interest. To date, Sato et al. (1996) has examined the intensity of white light that induces anthocyanin production in strawberry cell culture lines; however, no information was obtained about the effect of light quality on the production of anthocyanins in cultural cells. Therefore, the originality of this research is to investigate anthocyanin biosynthesis in cultural cells exposed to UV-B (290-320 nm) radiation.In this study, we used Rosa hybrida cultivar 'Charleston', a climbing type, originated in Australia. The open flowers of 'Charleston' undergo a striking color change from yellow to red over 10-12 days under natural daylight. We established a suspension cultured cell line from this cultivar of rose. Anthocyanin content was quantified by HPLC to determine the most efficient conditions, such as light including UV-B radiation and media, for continuous production of the pigments in the rose cell suspension. The expression levels of three genes encoding putative key enzymes (DFR, ANS, and UF3GT) for anthocyanin biosynthesis were ana...

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Section: Effects Of Medium Components and Light Irradiation On Anthocsupporting

confidence: 79%

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Hennayake

Takagi

Nishimura

et al. 2006

Plant Biotechnology

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“…After phase separation with diethylether to remove chlorophylls, the aqueous extract was analyzed for absorbance at wavelengths of 535.0, 650.0, and 750.0 nm using a Biochrom 4060 spectrophotometer (Pharmacia LKB Biochrom). Anthocyanin concentrations were then calculated using the extinction coefficient (E 1% 1cm 598:2 at l 5 535 nm) according to Do and Cormier (1991).…”

Section: Anthocyaninsmentioning

confidence: 99%

A Cellular Timetable of Autumn Senescence

et al. 2005

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We have studied autumn leaf senescence in a free-growing aspen (Populus tremula) by following changes in pigment, metabolite and nutrient content, photosynthesis, and cell and organelle integrity. The senescence process started on September 11, 2003, apparently initiated solely by the photoperiod, and progressed steadily without any obvious influence of other environmental signals. For example, after this date, senescing leaves accumulated anthocyanins in response to conditions inducing photooxidative stress, but at the beginning of September the leaves did not. Degradation of leaf constituents took place over an 18-d period, and, although the cells in each leaf did not all senesce in parallel, senescence in the tree as a whole was synchronous. Lutein and b-carotene were degraded in parallel with chlorophyll, whereas neoxanthin and the xanthophyll cycle pigments were retained longer. Chloroplasts in each cell were rapidly converted to gerontoplasts and many, although not all, cells died. From September 19, when chlorophyll levels had dropped by 50%, mitochondrial respiration provided the energy for nutrient remobilization. Remobilization seemed to stop on September 29, probably due to the cessation of phloem transport, but, up to abscission of the last leaves (over 1 week later), some cells were metabolically active and had chlorophyllcontaining gerontoplasts. About 80% of the nitrogen and phosphorus was remobilized, and on September 29 a sudden change occurred in the d 15 N of the cellular content, indicating that volatile compounds may have been released.

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“…Studies with cell cultures of various species find anthocyanin accumulation resulting from osmotic stress induced by glucose (74,75), sucrose (76-8 1) and mannitol (74,75,78,80,82). Dilution stress was also shown to induce anthocyanins in normally pigmen:-free Petunia cell cultures (83).…”

Section: Osmotic Inductionmentioning

confidence: 99%

“…Likewise, Woodall and Stewart (34) mention that anthocyanins acylated with phenolics such as cinnamic acid are able to absorb higher end UVB (310-320 nm), and the absence of these compounds was directly correlated with UVB damage in red cabbage seedlings (43). Some stresses have been shown to increase the methylation (78) or acylation (90) of anthocyanins. Obviously such alterations would impact the absorption spectrum of these compounds.…”

Section: Environmental Strain Reduction-photoprotectionmentioning

confidence: 99%

Environmental Significance of Anthocyanins in Plant Stress Responses

Chalker-Scott

1999

Photochem & Photobiology

1,281

386

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Anthocyanins are water-soluble pigments found in all plant tissues throughout the plant kingdom. Our understanding of anthocyanin biosynthesis and its molecular control has greatly improved in the last decade. The adaptive advantages of anthocyanins, especially in nonreproductive tissues, is much less clear. Anthocyanins often appear transiently at specific developmental stages and may be induced by a number of environmental factors including visible and UVB radiation, cold temperatures and water stress. The subsequent production and localization of anthocyanins in root, stem and especially leaf tissues may allow the plant to develop resistance to a number of environmental stresses. This article reviews the environmental induction of anthocyanins and their proposed importance in ameliorating environmental stresses induced by visible and UVB radiation, drought and cold temperatures.

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Accumulation of peonidin 3-glucoside enhanced by osmotic stress in grape (Vitis vinifera L.) cell suspension

Cited by 68 publications

References 21 publications

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

Differential expression of anthocyanin biosynthesis genes in suspension culture cells of Rosa hybrida cv. Charleston

A Cellular Timetable of Autumn Senescence

Environmental Significance of Anthocyanins in Plant Stress Responses

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