Accumulation of peroxidase-related reactive oxygen species in trichoblasts correlates with root hair initiation in barley

Kwaśniewski, Mirosław; Chwiałkowska, Karolina; Kwaśniewska, Jolanta; Kusak, Julia; Siwinski, Kamil; Szarejko, Iwona

doi:10.1016/j.jplph.2012.09.017

Cited by 44 publications

(31 citation statements)

References 29 publications

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“…The expression of both root hair-related peroxidase genes identified in Arabidopsis is root hair specific, and both contain RHEs in their proximal promoters that drive the root hairspecific expression of many root hair formation-related genes (Kim et al, 2006;Won et al, 2009). In our recent study, we demonstrated that the expression of both peroxidase genes identified in barley (as shown here, the putative orthologs of RHS18 and RHS19) is correlated with the initiation of root hair formation and is root hair specific, and both contain RHEs conserved within their orthologs in monocotyledonous species in their promoters (Kwasniewski et al, 2012). Therefore, the iRootHair resources can be used not only for the identification of primary root hair genes and their orthologous groups but also for secondary studies like comparative promoter analyses for the identification of conserved cis-acting elements that can act as putative functional regulatory sequences (Balazadeh et al, 2011).…”

Section: Discussionsupporting

confidence: 60%

iRootHair: A Comprehensive Root Hair Genomics Database

et al. 2012

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The specialized root epidermis cells of higher plants produce long, tubular outgrowths called root hairs. Root hairs play an important role in nutrient and water uptake, and they serve as a valuable model in studies of plant cell morphogenesis. More than 1,300 articles that describe the biological processes of these unique cells have been published to date. As new fields of root hair research are emerging, the number of new papers published each year and the volumes of new relevant data are continuously increasing. Therefore, there is a general need to facilitate studies on root hair biology by collecting, presenting, and sharing the available information in a systematic, curated manner. Consequently, in this paper, we present a comprehensive database of root hair genomics, iRootHair, which is accessible as a Web-based service. The current version of the database includes information about 153 root hair-related genes that have been identified to date in dicots and monocots along with their putative orthologs in higher plants with sequenced genomes. In order to facilitate the use of the iRootHair database, it is subdivided into interrelated, searchable sections that describe genes, processes of root hair formation, root hair mutants, and available references. The database integrates bioinformatics tools with a focus on sequence identification and annotation. iRootHair is a unique resource for root hair research that integrates the large volume of data related to root hair genomics in a single, curated, and expandable database that is freely available at www.iroothair.org.

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Section: Discussionsupporting

confidence: 60%

iRootHair: A Comprehensive Root Hair Genomics Database

et al. 2012

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“…The fluorescent probe 3 0 -(p-hydroxyphenyl) fluorescein (HPF) (Invitrogen, USA) was used to check ROS generation in roots as described by Kwasniewski et al (2013). Callose formation in roots was determined by treating with aniline blue for 1 h under dark conditions (Horst et al 1997).…”

Section: Microscopic Analysismentioning

confidence: 99%

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Nair

Chung

2017

3 Biotech

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Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of copper oxide nanoparticles (CuO NPs) on physiological, biochemical, and molecular indices in seedlings of an important oil seed crop Brassica napus L. The seedlings were treated with 0, 20, 50, 100, 200, 400, and 500 mg/L of CuO NPs for 14 days in halfstrength semi-solid Murashige and Skoog medium. The CuO NPs treatment significantly reduced shoot and root growth as well as plant biomass. Shortening and thickening of primary and lateral roots and inhibition of lateral root growth was observed at higher concentrations. An increase in reactive oxygen species generation, and malondialdehyde accumulation was observed. Histochemical staining of roots with propidium iodide and aniline blue indicated cell death and callose formation in roots. Transcriptional modulation of genes related to oxidative stress viz. CuZn superoxide dismutase, catalase, and ascorbate peroxidase was observed. Element content analysis showed an increase in Cu content and decrease in Fe, Mn, and Zn contents. Overall, exposure to CuO NPs caused oxidative injury, cell death, callose formation, and decreased the micro nutrient contents in B. napus seedlings.

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“…For detection of H 2 O 2 generation in roots, the fluorescent probe, 3′-(p-hydroxyphenyl) fluorescein (HPF) (Invitrogen, USA) was used [14]. The cell death in roots was determined using propidium iodide (PI) treatment with minor modifications from the method of Kwon et al [15].…”

Section: Detection Of Reactive Oxygen Species Cell Death and Ligninmentioning

confidence: 99%

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

Prakash¹,

Chung²

2016

Acta Biologica Hungarica

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The effect of zinc oxide nanoparticles (ZnONPs) was studied in wheat (Triticum aestivum L.) seedlings under in vitro exposure conditions. To avoid precipitation of nanoparticles, the seedlings were grown in half strength semisolid Murashige and Skoog medium containing 0, 50, 100, 200, 400 and 500 mg L -1 of ZnONPs. Analysis of zinc (Zn) content showed significant increase in roots. In vivo detection using fluorescent probe Zynpyr-1 indicated accumulation of Zn in primary and lateral root tips. All concentrations of ZnONPs significantly reduced root growth. However, significant decrease in shoot growth was observed only after exposure to 400 and 500 mg L -1 of ZnONPs. The reactive oxygen species and lipid peroxidation levels significantly increased in roots. Significant increase in cell-wall bound peroxidase activity was observed after exposure to 500 mg L -1 of ZnONPs. Histochemical staining with phloroglucinol-HCl showed lignification of root cells upon exposure to 500 mg L -1 of ZnONPs. Treatment with propidium iodide indicated loss of cell viability in root tips of wheat seedlings. These results suggest that redox imbalances, lignification and cell death has resulted in reduction of root growth in wheat seedlings exposed to ZnONPs nanoparticles.

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Accumulation of peroxidase-related reactive oxygen species in trichoblasts correlates with root hair initiation in barley

Cited by 44 publications

References 29 publications

iRootHair: A Comprehensive Root Hair Genomics Database

iRootHair: A Comprehensive Root Hair Genomics Database

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Determination of zinc oxide nanoparticles toxicity in root growth in wheat (Triticum aestivumL.) seedlings

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